scholarly journals Visualization and functional analysis of the oligomeric states of Escherichia coli heat shock protein 70 (Hsp70/DnaK)

2011 ◽  
Vol 17 (3) ◽  
pp. 313-327 ◽  
Author(s):  
Andrea D. Thompson ◽  
Steffen M. Bernard ◽  
Georgios Skiniotis ◽  
Jason E. Gestwicki
Keyword(s):  
Escherichia Coli ◽  
Functional Analysis ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Heat Shock Protein 70

scholarly journals Flagellin Contamination of Recombinant Heat Shock Protein 70 Is Responsible for Its Activity on T Cells

2006 ◽  
Vol 282 (7) ◽  
pp. 4479-4484 ◽  
Author(s):  
Zhiyong Ye ◽  
Yunn-Hwen Gan
Keyword(s):  
Escherichia Coli ◽  
T Cells ◽  
Heat Shock ◽  
Heat Shock Proteins ◽  
Heat Shock Protein ◽  
Heat Shock Protein 70 ◽  
Burkholderia Pseudomallei ◽  
Jurkat T Cells ◽  
Recombinant Hsp70 ◽  
Shock Proteins

Heat shock proteins (Hsp) 60 and 70 have been intensively studied for their ability to activate innate immunity. Heat shock proteins had been shown to induce the activation of dendritic cells, T cells, and B cells. However, the possible contamination of endotoxin in heat shock protein preparations makes their function as an activator of immune system ambiguous. Here, we examined the ability of bacterial Hsp60 and Hsp70 to activate Jurkat T cells and primary T cells. We found that Burkholderia pseudomallei Hsp70 and Mycobacterium tuberculosis Hsp70 could costimulate Jurkat T cells to make IL-2 and signal through TLR5. This costimulatory activity is not due to endotoxin or contaminants signaling via TLR2 nor TLR4. However, recombinant Hsp70 expressed in Escherichia coli ΔfliC strain completely lost its ability to costimulate T cells. Thus, the activation of T cells by recombinant Hsp70 is ascribed to flagellin contamination.

Escherichia coli-induced temporal and differential secretion of heat-shock protein 70 and interleukin-1β by human fetal membranes in a two-compartment culture system

Placenta ◽  
2015 ◽  
Vol 36 (3) ◽  
pp. 262-269 ◽  
Author(s):  
M. Osorio-Caballero ◽  
C. Perdigón-Palacio ◽  
G. García-López ◽  
O. Flores-Herrera ◽  
S. Olvera-Sánchez ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Heat Shock Protein 70 ◽  
Culture System ◽  
Interleukin 1Β ◽  
Fetal Membranes

Molecular cloning, characterization and functional analysis of a heat shock protein 70 gene in Cyclina sinensis

2016 ◽  
Vol 58 ◽  
pp. 663-668 ◽  
Author(s):  
Yipeng Ren ◽  
Heting Pan ◽  
Ying Yang ◽  
Baoping Pan ◽  
Wenjun Bu
Keyword(s):  
Functional Analysis ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Molecular Cloning ◽  
Heat Shock Protein 70 ◽  
Cyclina Sinensis

Ammonia aggravates emotional stress-induced gastric mucosal oxidative injury through the cancellation of heat shock protein 70 (HSP70)

2001 ◽  
Vol 120 (5) ◽  
pp. A152-A152
Author(s):  
H SUZUKI ◽  
S NAGAHASHI ◽  
M MIYAZAWA ◽  
M MORI ◽  
H NAGATA ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Emotional Stress ◽  
Heat Shock Protein 70 ◽  
Oxidative Injury
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