Statistical Analysis of the Laboratory Methods Used to Detect Potato Virus Y

2009 ◽  
Vol 86 (4) ◽  
pp. 265-271 ◽  
Author(s):  
Yuliya V. Bolotova ◽  
Alexander V. Karasev ◽  
Christopher S. McIntosh
Keyword(s):  
Statistical Analysis ◽  
Potato Virus ◽  
Potato Virus Y ◽  
Laboratory Methods

scholarly journals MONITORING STUDIES OF VIRAL DISEASES IN POTATOES PLANTINGS OF UKRAINIAN MARSHY WOODLANDS

2012 ◽  
Vol 15 ◽  
pp. 140-149
Author(s):  
O.A. Dmitruk ◽  
Yu.A. Dmitruk ◽  
T.A. Bova ◽  
A.V. Pirog ◽  
L.P. Kolomiets
Keyword(s):  
Viral Infection ◽  
Potato Virus ◽  
Potato Virus Y ◽  
Viral Diseases ◽  
Latent Infections ◽  
Viral Control ◽  
Laboratory Methods ◽  
Seed Material ◽  
Original Seed ◽  
Efficient Protection

The examinations of potatoes original seed nursery in north regions of Ukrainian marshy woodlands in 2012 (47 sorts) were performed. It was revealed that spreading of viral infection and diseases symptoms onset on potatoes cultivars increases with the number of field reproductions from 8,1 % (tube plants – seedlings) to 77–100 %. The wide-spreading of latent infections was observed – 57 % of tested samples. Potato virus М prevails in plantings as monoinfection (36,2 %) or in complex with other mosaic viruses (99 %), Potato virus Y was detected in 38,3 % of cultivars, with domination of necrotic isolates. The need of efficient protection and constant viral control of seed material with the use of laboratory methods and modern diagnostic facilities is emphasized.

scholarly journals The effect of Ethrel on detection of potato virus Y (PVYº) in primarily infected potato plants

2013 ◽  
Vol 41 (2) ◽  
pp. 257-264
Author(s):  
Marian Staszewicz
Keyword(s):  
Statistical Analysis ◽  
Potato Virus ◽  
Active Ingredient ◽  
Potato Virus Y ◽  
Elisa Test ◽  
Field Conditions ◽  
Average Deviation ◽  
Potato Plants ◽  
Control Variant ◽  
And Control

The effect of ethylene (in the form of Ethrel) on the detection of PVYº in cv. Kaszubski potatoes primarily infected in field conditions, was studied. The plants were inoculated with PVYº and sprayed with Ethrel at a concentration of 100 mg/dm<sup>3</sup>, 10 cm<sup>3</sup> of solution were used per plant. The concentration of the active ingredient, CEPA, in this amount of solution was 1 mg/plant. The extinction value and PVYº detection were assessed in the leaf and tuber juices from Ethrel-treated plants and control variant. The ELISA test was used. The results were subjected to statistical analysis and the average deviation at <i>P</i> = 0.05 was calculated. It was shown that Ethrel lowered the value of extinction and decreased PVYº detection in the leaves and tubers of potato plants of the Kaszubski variety primarily infected in field conditions.

Expression of the Tomato pot-1 Gene Confers Potato Virus Y (PVY) Resistance in Susceptible Potato Varieties

2021 ◽  
Author(s):  
Chen Zhang ◽  
Kelly A. Zarka ◽  
Daniel G. Zarka ◽  
Jonathan L. Whitworth ◽  
David S. Douches
Keyword(s):  
Potato Virus ◽  
Potato Virus Y ◽  
Potato Varieties ◽  
Pvy Resistance

Transmission of potato virus Y byRhopalosiphum padi L.

1979 ◽  
Vol 22 (3) ◽  
pp. 237-238 ◽  
Author(s):  
Michal Kostiw
Keyword(s):  
Potato Virus ◽  
Potato Virus Y

scholarly journals Monitoring Current-Season Spread of Potato virus Y in Potato Fields Using ELISA and Real-Time RT-PCR

Plant Disease ◽  
2013 ◽  
Vol 97 (5) ◽  
pp. 641-644 ◽  
Author(s):  
Manphool S. Fageria ◽  
Mathuresh Singh ◽  
Upeksha Nanayakkara ◽  
Yvan Pelletier ◽  
Xianzhou Nie ◽  
...  
Keyword(s):  
Real Time ◽  
Potato Virus ◽  
Potato Virus Y ◽  
Enzyme Linked Immunosorbent Assay ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Current Season ◽  
Seed Market ◽  
Polymerase Chain ◽  
Time Of Harvest

The current-season spread of Potato virus Y (PVY) was investigated in New Brunswick, Canada, in 11 potato fields planted with six different cultivars in 2009 and 2010. In all, 100 plants selected from each field were monitored for current-season PVY infections using enzyme-linked immunosorbent assay (ELISA) and real-time reverse-transcription polymerase chain reaction (RT-PCR) assay. Average PVY incidence in fields increased from 0.6% in 2009 and 2% in 2010 in the leaves to 20.3% in 2009 and 21.9% in 2010 in the tubers at the time of harvest. In individual fields, PVY incidence in tubers reached as high as 37% in 2009 and 39% in 2010 at the time of harvest. Real-time RT-PCR assay detected more samples with PVY from leaves than did ELISA. A higher number of positive samples was also detected with real-time RT-PCR from growing tubers compared with the leaves collected from the same plant at the same sampling time. PVY incidence determined from the growing tubers showed a significant positive correlation with the PVY incidence of tubers after harvest. Preharvest testing provides another option to growers to either top-kill the crop immediately to secure the seed market when the PVY incidence is low or leave the tubers to develop further for table or processing purposes when incidence of PVY is high.

Sign in / Sign up

Export Citation Format

Share Document