Expression of the Tomato pot-1 Gene Confers Potato Virus Y (PVY) Resistance in Susceptible Potato Varieties

2021 ◽  
Author(s):  
Chen Zhang ◽  
Kelly A. Zarka ◽  
Daniel G. Zarka ◽  
Jonathan L. Whitworth ◽  
David S. Douches
Keyword(s):  
Potato Virus ◽  
Potato Virus Y ◽  
Potato Varieties ◽  
Pvy Resistance

scholarly journals Virus elimination in potato through meristem culture followed by thermotherapy

2014 ◽  
Vol 11 (1) ◽  
pp. 71-80 ◽  
Author(s):  
MA Ali ◽  
KM Nasiruddin ◽  
MS Haque ◽  
SM Faisal
Keyword(s):  
Gibberellic Acid ◽  
Potato Virus ◽  
Potato Virus Y ◽  
Combined Treatment ◽  
Meristem Culture ◽  
Virus Elimination ◽  
Potato Varieties ◽  
Virus Indexing ◽  
Plantlet Production ◽  
Benzyl Amino Purine

Virus elimination in potato through meristem culture followed by thermotherapy and virus indexing was studied. Three levels of thermotherapy, viz. 27±1°C (control), 30±1°C and 35±1°C, sixteen combinations of BAP (Benzyl Amino Purine) plus GA3 (gibberellic Acid) concentrations viz. 0.0+0.0 (control), 0.0+0.2, 0.0+0.4, 0.0+0.6, 1.5+0.0, 1.5+0.2, 1.5+0.4, 1.5+0.6, 3.0+0.0, 3.0+0.2, 3.0+0.4, 3.0+0.6 , 4.5+0.0, 4.5+ 0.2, 4.5+0.4 and 4.5+0.6 were used in this study in three potato varieties viz. Diamant, Heera and Lalpakri. Among the thermo therapies, 27±1°C showed the highest (24.55) survival response followed by 30±1°C, 35±1°C, respectively. The poorest (20.47) survival response of meristem derived plantlets was noticed in 35±1°C which gave the highest percentage (43.79) of virus free plantlets followed by 30±1°C. The best (25.85%) survival response was found in Lalpakri and the lowest (19.08%) survivality was recorded in Diamant. The highest (33.27) percentage of PVY (Potato Virus Y) free plantlets was observed in Heera. The combined treatment 3.0 mg L-1 BAP and 0.2 mg L-1 GA3 showed the highest (63.39) percentage of virus free plantlet production followed by 4.5 mg L-1 BAP and 0.2 mg L-1 GA3DOI: http://dx.doi.org/10.3329/sja.v11i1.18376 SAARC J. Agri., 11(1): 71-80 (2013)

scholarly journals Strain-Specific Hypersensitive and Extreme Resistance Phenotypes Elicited by Potato virus Y Among 39 Potato Cultivars Released in Three World Regions Over a 117-Year Period

Plant Disease ◽  
2018 ◽  
Vol 102 (1) ◽  
pp. 185-196 ◽  
Author(s):  
Roger A. C. Jones ◽  
Stuart J. Vincent
Keyword(s):  
Potato Virus ◽  
Potato Virus Y ◽  
Secondary Infection ◽  
Potato Cultivars ◽  
Symptom Expression ◽  
Extreme Resistance ◽  
Potato Plants ◽  
Pvy Resistance ◽  
World Regions ◽  
Healthy Seed

Strain-specific hypersensitive (HR) and extreme resistance (ER) phenotypes elicited in potato plants by three Potato virus Y (PVY) isolates in strain groups PVYO (BL and DEL3) and PVYD (KIP1) were studied. PVYO and PVYD isolates elicit HR genes Ny or putative Nd, respectively, and all three isolates elicit ER gene Ry. They were inoculated to 39 Australasian, European, or North American potato cultivars released over a 117-year period and harvested tubers were replanted. Both primary and secondary symptoms were recorded. Two European cultivars always developed ER following sap and graft inoculation and, thus, carried comprehensive PVY resistance gene Ry. One Australasian and two European cultivars always developed susceptible phenotypes and, thus, lacked genes Ry, Ny, and putative Nd. Sap inoculation with isolate KIP1 elicited localized HR (LHR) in 31 cultivars and both LHR and systemic HR (SHR) in three others; thus, all carried putative Nd. Isolates BL and DEL3 both elicited susceptible phenotypes in 11 of these 34 cultivars but LHR alone, SHR alone, or both LHR and SHR in the other 23 which, therefore, all carry Ny. With these two isolates, SHR expression ranged from very severe to very weak, with the greatest numbers of isolate–cultivar combinations occurring in the severe category with BL (n = 11) and moderate category (n = 12) with DEL3. Within the same isolate–cultivar combination, overall, SHR symptom expression was weaker with secondary than primary infection. With both primary and secondary infection, SHR expression was most severe with KIP1 and weakest with DEL3. Genes Ny and putative Nd were present in cultivars released between 1939 and 2010 or 1893 and 2010, respectively, occurring in cultivars from all three world regions. These findings have important implications concerning breeding new PVY-resistant potato cultivars, especially for countries lacking healthy seed potato stocks, or where subsistence farmers cannot afford them. An alternative to including gene Ry is incorporating as many strain-specific PVY resistance genes as possible.

scholarly journals The effect of potato rugose on the yield of some potato varieties

1961 ◽  
Vol 33 (1) ◽  
pp. 89-100
Author(s):  
Onni Pohjakallio ◽  
Laura Karhuvaara ◽  
Simo Antila
Keyword(s):  
Virus Infection ◽  
Potato Virus ◽  
Potato Plant ◽  
Potato Virus Y ◽  
Tuber Yield ◽  
Virus Disease ◽  
Potato Varieties ◽  
Potato Plants ◽  
Severe Type

The virus disease occurring in the potato varieties Harbinger, Aquila, and King George V was probably the potato rugose caused by potato virus Y (Solanum virus 2). In the variety Harbinger, the degree of strength of the virus disease probably varied as a result of the appearance of different races of potato virus Y. The type of the virus disease did not change from one vegetative generation of the potato to another. In the potato varieties Aquila and King George V, only the severe type of the disease was observed. The severe type of the virus disease reduced the tuber yield of all the potato varieties investigated to a level of 5 to 6 %, and the slight type occurring in the variety Harbinger to an average of 27 % of the normal. The virus infection appeared more frequently in Harbinger than in the variety Aquila. In the summer, when the infection of the potato plant took place, the virus disease reduced its productivity only relatively little. In the following vegetative generation of the diseased potato plants, the degree of their degeneration conclusively displayed itself. Following this, the wakening of the virus diseased plants did not progress further from one vegetative generation to the next.

scholarly journals Characterization of a Novel Resistance-Breaking Isolate of Potato Virus Y in Nicotiana Tabacum

2014 ◽  
Vol 3 (1) ◽  
pp. 01-10
Author(s):  
Jae-Hyun Kim ◽  
Young-Soo Kim ◽  
Soo-Won Jang ◽  
Yong-Ho Jeon
Keyword(s):  
Potato Virus ◽  
Potato Virus Y ◽  
Biological Properties ◽  
Systemic Movement ◽  
Pvy Resistance ◽  
Resistance Breaking ◽  
Systemic Vein ◽  
Vein Necrosis ◽  
Tobacco Cultivar

Potato virus Y (PVY) was identified from a PVY-resistance flue-cured tobacco variety KF120 showing vein necrosis at Buron province, Korea. Biological properties of the isolate named PVY-ToBR1 was characterized using various host plants with another isolate PVY-ToJC37. The isolated PVY-ToBR1 induced systemic vein necrosis symptoms on a PVY-resistant tobacco cultivar (VAM) harboring potyvirus resistant va gene, though tissue printing showed the systemic movement of virus was slightly delayed. By contrast, the isolate PVY-ToJC37 failed to infect VAM plants and the virus was not detected on inoculated leaf and systemic leaves in VAM plants. Similarly, the isolated PVY-ToBR1 induced distinctly systemic vein necrosis symptoms on PVY-resistant tobacco cultivars (V.SCR, PBD6, TN86, TN90, Virgin A Mutant, NC744, and Wislica) that have the recessive potyvirus resistance gene va, but PVY-ToJC37 did not infect systemically infect these tobacco cultivars, suggesting that PVY-ToBR1 is a novel resistance-breaking isolate in tobacco. The coat protein (CP) genes of PVY-ToBR1 and PVY-ToJC37 were amplified using RT-PCR assays with specific primers for PVY isolates and nucleotide sequences of the CP genes were determined. The isolate PVY-ToBR1 showed 88.4% - 99.4% and 86.6% - 99.4% CP identities to the 46 different PVY isolates at the nucleotide and amino acid, respectively. Phylogenetic relationship from CP comparisons showed that PVY-ToBR1 isolate clustered with PVYNTN isolates and PVY-ToBR1 isolate more closely related to the isolates from European than from North American PVY NTN.

THE REACTIONS OF CERTAIN SOLANACEOUS SPECIES TO STRAINS OF POTATO VIRUS Y

1955 ◽  
Vol 33 (4) ◽  
pp. 355-361 ◽  
Author(s):  
James Munro
Keyword(s):  
Potato Virus ◽  
Potato Virus Y ◽  
Avirulent Strain ◽  
Potato Varieties ◽  
Nicotiana Species ◽  
Solanaceous Species ◽  
Leaf Drop ◽  
Young Leaves

Strains of potato virus Y that: were not clearly differentiated from each other by their reaction on potato varieties were shown to be separate entities by the differing reactions that they caused in several other solanaceous species. One strain did not infect Physalis floridana, but this same strain caused necrosis and a severe distortion of the young leaves of Nicotiana sanderae. After infection with certain other strains, N. rustica showed leaf-drop streak symptoms; this observation has not been previously reported. An avirulent strain in all potato varieties and seedlings tested so far caused a severely necrotic disease in N. tabacum and other Nicotiana species.

scholarly journals Field Evaluation and Enzyme-Linked Immunosorbent Assay Detection of Potato Leaf Roll Virus, Potato Virus X and Potato Virus Y in Potato Germplasm

2017 ◽  
Vol 9 (7) ◽  
pp. 229 ◽  
Author(s):  
Romana Anjum ◽  
M. Aslam Khan ◽  
Kolawole Oluwaseun Olawale ◽  
Raheel Baber
Keyword(s):  
Immunosorbent Assay ◽  
Potato Virus ◽  
Potato Virus Y ◽  
Rating Scale ◽  
Leaf Roll ◽  
Potato Virus X ◽  
Potato Leaf Roll Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Potato Leaf ◽  
Potato Varieties

Polerovirus: potato leaf roll virus (PLRV), Potyvirus: potato virus Y (PVY) and Potexvirus: potato virus X (PVX) is more destructive and well distributed throughout the Pakistan. Incidence has been reported to be as high as 90%, 25%, and ≥ 15%, respectively in the potato growing regions. To find out the source of resistance, twenty-nine virus free potato varieties were grown under field conditions with good agricultural practices. The disease severity of PLRV, PVY and PVX was recorded to determine the level of resistance of the potato varieties according to the disease rating scale. Infectivity and biological assay of all twenty-nine varieties were done in green house on potato, Datura stramonium, Nicotiana glutinosa and Physalis floridana. Non-inoculated plants were served as control. Leaf samples from potato varieties were collected for serological detection of PLRV, PVY and PVX by Double antibody sandwich Enzyme-Linked Immunosorbent Assay (DAS-ELISA). Out of twenty nine varieties, none of the variety was resistant to PLRV although three varieties; Mirrato, 394021-120 and Orla were moderately resistant. Only FD 48-4 and TPS 9813 showed resistance to PVX and PVY. While FD 3-10, FD 9616 and FD 37-13 were moderately to PVX and PVY. Rest of the varieties was found susceptible to all three viruses.

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