Improvement of soluble recombinant interferon-α expression by methyl α-D-glucopyranoside in araBAD promoter system of Escherichia coli

2009 ◽  
Vol 14 (3) ◽  
pp. 274-280 ◽  
Author(s):  
Kyung-Hwan Jung ◽  
You-Jin Lee ◽  
Ji-Hyeon Yeon ◽  
Sun Kyun Yoo ◽  
Byeong-Churl Chung
Keyword(s):  
Escherichia Coli ◽  
Interferon Α ◽  
Recombinant Interferon ◽  
Promoter System

Improving the yield of soluble 6xHis-tagged interferon-α via the addition of repressor of the araBAD promoter system in Escherichia coli

2008 ◽  
Vol 30 (9) ◽  
pp. 1577-1582 ◽  
Author(s):  
Kyung-Hwan Jung ◽  
Young-Seo Park ◽  
Ji-Hyeon Yeon ◽  
Sung-Hun Kim ◽  
Sun Kyun Yoo ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Interferon Α ◽  
Promoter System

Allergy of delayed type to recombinant interferon α 2c

1989 ◽  
Vol 20 (2) ◽  
pp. 149-150 ◽  
Author(s):  
Ulf Detmar ◽  
Monika Agathos ◽  
Christoph Nerl
Keyword(s):  
Interferon Α ◽  
Recombinant Interferon

Characterization of the T7 promoter system for expressing penicillin acylase in Escherichia coli

2006 ◽  
Vol 72 (3) ◽  
pp. 529-536 ◽  
Author(s):  
Yali Xu ◽  
Stefan Rosenkranz ◽  
Chiao-Ling Weng ◽  
Jeno M. Scharer ◽  
Murray Moo-Young ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Penicillin Acylase ◽  
T7 Promoter ◽  
Promoter System

scholarly journals Effect of Carotenoids, Oligosaccharides and Anthocyanins on Growth Performance, Immunological Parameters and Intestinal Morphology in Broiler Chickens Challenged with Escherichia coli Lipopolysaccharide

Animals ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 347 ◽  
Author(s):  
Brigitta Csernus ◽  
Sándor Biró ◽  
László Babinszky ◽  
István Komlósi ◽  
András Jávor ◽  
...  
Keyword(s):  
Gene Expression ◽  
Escherichia Coli ◽  
Mrna Expression ◽  
Intestinal Morphology ◽  
Interferon Α ◽  
Toll Like Receptor ◽  
Lps Challenge ◽  
Toll Like Receptor 5 ◽  
Relative Mrna Expression ◽  
Escherichia Coli Lipopolysaccharide

This study was conducted to investigate the effect of carotenoid, oligosaccharide and anthocyanin supplementation in broiler diets under Escherichia coli lipopolysaccharide (LPS) challenge. Ross 308 chickens were fed 5 diets: basal diet (control diet), diet supplemented with β-glucan in 0.05% (positive control) and diets with 0.5% carotenoid-, oligosaccharide- or anthocyanin contents. On the 26th days of age, chickens were challenged intraperitoneally 2 mg LPS per kg of body weight. 12 h after injection, birds were euthanized, then spleen and ileum samples were collected. LPS induced increased relative mRNA expression of splenic (p = 0.0445) and ileal (p = 0.0435) interleukin-1β (IL-1β), which was lower in the spleen in carotenoid (p = 0.0114), oligosaccharide (p = 0.0497) and anthocyanin (p = 0.0303)-treated chickens compared to LPS-injected control birds. Dietary supplementation of carotenoids also decreased relative gene expression of splenic interleukin-6 (IL-6) (p = 0.0325). In the ileum, β-glucan supplementation showed lower relative mRNA expression of toll-like receptor 5 (TLR-5) (p = 0.0387) compared to anthocyanin treatment. Gene expression of both splenic and ileal interferon-α (IFN-α), interferon-γ (IFN-γ), toll-like receptor 4 (TLR-4) and toll-like receptor 5 (TLR-5) were not influenced by dietary supplements. In conclusion, carotenoids, oligosaccharides and anthocyanins could partially mitigate the immune stress caused by LPS challenge. All of the compounds impacted longer villus height (p < 0.0001), villus height:crypt depth ratios were higher after β-glucan (p < 0.0001) and anthocyanin (p = 0.0063) supplementations and thickened mucosa was observed in β-glucan (p < 0.0001), oligosaccharide (p < 0.0001) and anthocyanin (p = 0.048) treatments. All of these findings could represent a more effective absorption of nutrients.

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