scholarly journals Decoding Cortical Glial Cell Development

2021 ◽  
Author(s):  
Xiaosu Li ◽  
Guoping Liu ◽  
Lin Yang ◽  
Zhenmeiyu Li ◽  
Zhuangzhi Zhang ◽  
...  
Keyword(s):  
Single Cell ◽  
Glial Cell ◽  
Glial Cells ◽  
Cell Lineage ◽  
Gabaergic Interneurons ◽  
Rna Seq ◽  
Radial Glial Cells ◽  
Intermediate Progenitors ◽  
Late Embryogenesis ◽  
Radial Glial

AbstractMouse cortical radial glial cells (RGCs) are primary neural stem cells that give rise to cortical oligodendrocytes, astrocytes, and olfactory bulb (OB) GABAergic interneurons in late embryogenesis. There are fundamental gaps in understanding how these diverse cell subtypes are generated. Here, by combining single-cell RNA-Seq with intersectional lineage analyses, we show that beginning at around E16.5, neocortical RGCs start to generate ASCL1+EGFR+ apical multipotent intermediate progenitors (MIPCs), which then differentiate into basal MIPCs that express ASCL1, EGFR, OLIG2, and MKI67. These basal MIPCs undergo several rounds of divisions to generate most of the cortical oligodendrocytes and astrocytes and a subpopulation of OB interneurons. Finally, single-cell ATAC-Seq supported our model for the genetic logic underlying the specification and differentiation of cortical glial cells and OB interneurons. Taken together, this work reveals the process of cortical radial glial cell lineage progression and the developmental origins of cortical astrocytes and oligodendrocytes.

scholarly journals Mind Bomb 1-Expressing Intermediate Progenitors Generate Notch Signaling to Maintain Radial Glial Cells

Neuron ◽  
2008 ◽  
Vol 58 (4) ◽  
pp. 519-531 ◽  
Author(s):  
Ki-Jun Yoon ◽  
Bon-Kyoung Koo ◽  
Sun-Kyoung Im ◽  
Hyun-Woo Jeong ◽  
Jaewang Ghim ◽  
...  
Keyword(s):  
Notch Signaling ◽  
Glial Cells ◽  
Radial Glial Cells ◽  
Intermediate Progenitors ◽  
Radial Glial

scholarly journals Persistent expression of stabilized β-catenin delays maturation of radial glial cells into intermediate progenitors

2007 ◽  
Vol 309 (2) ◽  
pp. 285-297 ◽  
Author(s):  
Carolyn N. Wrobel ◽  
Christopher A. Mutch ◽  
Sruthi Swaminathan ◽  
Makoto M. Taketo ◽  
Anjen Chenn
Keyword(s):  
Glial Cells ◽  
Radial Glial Cells ◽  
Intermediate Progenitors ◽  
Radial Glial

scholarly journals Gliogenic Potential of Single Pallial Radial Glial Cells in Lower Cortical Layers

Cells ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 3237
Author(s):  
Ana Cristina Ojalvo-Sanz ◽  
Laura López-Mascaraque
Keyword(s):  
Glial Cell ◽  
Glial Cells ◽  
Lineage Tracing ◽  
Genetic Lineage ◽  
Neural Cells ◽  
Cell Type ◽  
Cortical Layers ◽  
Radial Glial Cells ◽  
Radial Glial

During embryonic development, progenitor cells are progressively restricted in their potential to generate different neural cells. A specific progenitor cell type, the radial glial cells, divides symmetrically and then asymmetrically to produce neurons, astrocytes, oligodendrocytes, and NG2-glia in the cerebral cortex. However, the potential of individual progenitors to form glial lineages remains poorly understood. To further investigate the cell progeny of single pallial GFAP-expressing progenitors, we used the in vivo genetic lineage-tracing method, the UbC-(GFAP-PB)-StarTrack. After targeting those progenitors in embryonic mice brains, we tracked their adult glial progeny in lower cortical layers. Clonal analyses revealed the presence of clones containing sibling cells of either a glial cell type (uniform clones) or two different glial cell types (mixed clones). Further, the clonal size and rostro-caudal cell dispersion of sibling cells differed depending on the cell type. We concluded that pallial E14 neural progenitors are a heterogeneous cell population with respect to which glial cell type they produce, as well as the clonal size of their cell progeny.

scholarly journals Transcriptional priming as a conserved mechanism of lineage diversification in the developing mouse and human neocortex

2020 ◽  
Vol 6 (45) ◽  
pp. eabd2068
Author(s):  
Zhen Li ◽  
William A. Tyler ◽  
Ella Zeldich ◽  
Gabriel Santpere Baró ◽  
Mayumi Okamoto ◽  
...  
Keyword(s):  
Glial Cells ◽  
Radial Glia ◽  
Radial Glial Cells ◽  
Human Neocortex ◽  
Intermediate Progenitors ◽  
Cell Diversity ◽  
Radial Glial ◽  
The Rich ◽  
Lineage Diversification

How the rich variety of neurons in the nervous system arises from neural stem cells is not well understood. Using single-cell RNA-sequencing and in vivo confirmation, we uncover previously unrecognized neural stem and progenitor cell diversity within the fetal mouse and human neocortex, including multiple types of radial glia and intermediate progenitors. We also observed that transcriptional priming underlies the diversification of a subset of ventricular radial glial cells in both species; genetic fate mapping confirms that the primed radial glial cells generate specific types of basal progenitors and neurons. The different precursor lineages therefore diversify streams of cell production in the developing murine and human neocortex. These data show that transcriptional priming is likely a conserved mechanism of mammalian neural precursor lineage specialization.

scholarly journals Developmental Origins of Human Cortical Oligodendrocytes and Astrocytes

2021 ◽  
Author(s):  
Lin Yang ◽  
Zhenmeiyu Li ◽  
Guoping Liu ◽  
Xiaosu Li ◽  
Zhengang Yang
Keyword(s):  
Olfactory Bulb ◽  
Glial Cells ◽  
Pyramidal Neurons ◽  
Human Cortex ◽  
General Picture ◽  
Radial Glial Cells ◽  
Intermediate Progenitors ◽  
Molecular Features ◽  
Gestational Week ◽  
Radial Glial

AbstractHuman cortical radial glial cells are primary neural stem cells that give rise to cortical glutaminergic projection pyramidal neurons, glial cells (oligodendrocytes and astrocytes) and olfactory bulb GABAergic interneurons. One of prominent features of the human cortex is enriched with glial cells, but there are major gaps in understanding how these glial cells are generated. Herein, by integrating analysis of published human cortical single-cell RNA-Seq datasets with our immunohistochemistical analyses, we show that around gestational week 18, EGFR-expressing human cortical truncated radial glial cells (tRGs) give rise to basal multipotent intermediate progenitors (bMIPCs) that express EGFR, ASCL1, OLIG2 and OLIG1. These bMIPCs undergo several rounds of mitosis and generate cortical oligodendrocytes, astrocytes and olfactory bulb interneurons. We also characterized molecular features of the cortical tRG. Integration of our findings suggests a general picture of the lineage progression of cortical radial glial cells, a fundamental process of the developing human cerebral cortex.

scholarly journals Neuronal Regeneration from Ependymo-Radial Glial Cells: Cook, Little Pot, Cook!

2015 ◽  
Vol 32 (4) ◽  
pp. 516-527 ◽  
Author(s):  
Catherina G. Becker ◽  
Thomas Becker
Keyword(s):  
Glial Cells ◽  
Neuronal Regeneration ◽  
Radial Glial Cells ◽  
Radial Glial

scholarly journals Single-Cell RNA Sequencing in Human Retinal Degeneration Reveals Distinct Glial Cell Populations

Cells ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 438 ◽  
Author(s):  
Andrew P. Voigt ◽  
Elaine Binkley ◽  
Miles J. Flamme-Wiese ◽  
Shemin Zeng ◽  
Adam P. DeLuca ◽  
...  
Keyword(s):  
Single Cell ◽  
Retinal Degeneration ◽  
Rna Sequencing ◽  
Glial Cell ◽  
Glial Cells ◽  
Peripheral Retina ◽  
Cell Populations ◽  
Specific Gene ◽  
Autoimmune Retinopathy ◽  
Single Cell Rna Sequencing

Degenerative diseases affecting retinal photoreceptor cells have numerous etiologies and clinical presentations. We clinically and molecularly studied the retina of a 70-year-old patient with retinal degeneration attributed to autoimmune retinopathy. The patient was followed for 19 years for progressive peripheral visual field loss and pigmentary changes. Single-cell RNA sequencing was performed on foveal and peripheral retina from this patient and four control patients, and cell-specific gene expression differences were identified between healthy and degenerating retina. Distinct populations of glial cells, including astrocytes and Müller cells, were identified in the tissue from the retinal degeneration patient. The glial cell populations demonstrated an expression profile consistent with reactive gliosis. This report provides evidence that glial cells have a distinct transcriptome in the setting of human retinal degeneration and represents a complementary clinical and molecular investigation of a case of progressive retinal disease.

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