Development and validation of a highly sensitive second derivative synchronous fluorescence spectroscopic method for the simultaneous determination of elbasvir and grazoprevir in pharmaceutical preparation and human plasma

2020 ◽  
Vol 44 (43) ◽  
pp. 18679-18685
Author(s):  
Khalid A. M. Attia ◽  
Ahmed El-Olemy ◽  
Sherif Ramzy ◽  
Ahmed H. Abdelazim ◽  
Mohamed A. Hasan ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Simultaneous Determination ◽  
Human Plasma ◽  
Pharmaceutical Preparation ◽  
Spectroscopic Method ◽  
Second Derivative ◽  
Highly Sensitive ◽  
Development And Validation

Elbasvir and grazoprevir combination has been newly approved for the treatment of patients infected with hepatitis C virus.

scholarly journals Second Derivative Synchronous Fluorescence Spectroscopy for the Simultaneous Determination of Metoclopramide and Pyridoxine in Syrup and Human Plasma

2008 ◽  
Vol 91 (3) ◽  
pp. 542-550 ◽  
Author(s):  
Nahed El-Enany
Keyword(s):  
Simultaneous Determination ◽  
Human Plasma ◽  
High Sensitivity ◽  
Second Derivative ◽  
Native Fluorescence ◽  
Highly Sensitive ◽  
Experimental Parameters ◽  
The Mean ◽  
Good Agreement

Abstract A rapid, simple, and highly sensitive second derivative synchronous fluorometric method has been developed for the simultaneous determination of metoclopramide (MT) and pyridoxine (PY) in a binary mixture. The method is based on measurement of the native fluorescence of these drugs at = 80 nm in methanol. The different experimental parameters affecting the native fluorescence of the drugs were carefully studied and optimized. The fluorescence-concentration plots were rectilinear over the ranges of 0.020.4 and 0.12 g/mL for MT and PY, respectively. The limits of detection were 0.003 and 0.007 g/mL and the limits of quantification were 0.008 and 0.02 g/mL for MT and PY, respectively. The proposed method was successfully applied to the determination of MT and PY in synthetic mixtures and in commercial syrup. The results were in good agreement with those obtained with a reported method. The high sensitivity attained by the proposed method allowed the determination of MT in spiked and real human plasma samples. The mean percent recoveries of MT from spiked and real human plasma (n = 3) were 93.72 3.15 and 89.72 2.19, respectively.

Development and validation of a methodology based on Captiva EMR-lipid clean-up and LC-MS/MS analysis for the simultaneous determination of mycotoxins in human plasma

Talanta ◽  
2020 ◽  
Vol 206 ◽  
pp. 120193 ◽  
Author(s):  
Beatriz Arce-López ◽  
Elena Lizarraga ◽  
Myra Flores-Flores ◽  
Ángel Irigoyen ◽  
Elena González-Peñas
Keyword(s):  
Simultaneous Determination ◽  
Human Plasma ◽  
Ms Analysis ◽  
Development And Validation

Development and validation of a rapid HPLC method for simultaneous determination of tramadol, and its two main metabolites in human plasma

2006 ◽  
Vol 830 (2) ◽  
pp. 207-211 ◽  
Author(s):  
Mohammad-Reza Rouini ◽  
Yalda Hosseinzadeh Ardakani ◽  
Faezeh Soltani ◽  
Hassan Y. Aboul-Enein ◽  
Alireza Foroumadi
Keyword(s):  
Simultaneous Determination ◽  
Human Plasma ◽  
Hplc Method ◽  
Development And Validation
Sign in / Sign up

Export Citation Format

Share Document