Development and Validation of highly Sensitive Stability Indicating Spectrofluorimetric Method for Determination of Amlodipine in Pharmaceutical Preparations and Human Plasma

2016 ◽  
Vol 26 (6) ◽  
pp. 2141-2149 ◽  
Author(s):  
Abdel-Maaboud I. Mohamed ◽  
Mahmoud A. Omar ◽  
Mohamed A. Hammad ◽  
Abobakr A. Mohamed
Keyword(s):  
Human Plasma ◽  
Pharmaceutical Preparations ◽  
Stability Indicating ◽  
Spectrofluorimetric Method ◽  
Highly Sensitive ◽  
Development And Validation

Development and validation of a stability-indicating spectrofluorimetric method for the determination of H1N1 antiviral drug (oseltamivir phosphate) in human plasma through the Hantzsch reaction

RSC Advances ◽  
2015 ◽  
Vol 5 (35) ◽  
pp. 27735-27742 ◽  
Author(s):  
Mahmoud A. Omar ◽  
Sayed M. Derayea ◽  
Islam M. Mostafa
Keyword(s):  
Human Plasma ◽  
Antiviral Drug ◽  
Dosage Forms ◽  
Hantzsch Reaction ◽  
Pharmaceutical Dosage Forms ◽  
Stability Indicating ◽  
Spectrofluorimetric Method ◽  
Development And Validation ◽  
Oseltamivir Phosphate

A simple and sensitive spectrofluorimetric method has been described and validated for the determination of oseltamivir phosphate (OSP) in its pure form and pharmaceutical dosage forms.

Micellar-Enhanced Spectrofluorimetric Method for Quantification of Diclofenac Potassium in Pure Form, in Pharmaceutical Preparations and Human Plasma

2021 ◽  
Vol 58 (6) ◽  
pp. 427-434
Author(s):  
Muhammad Naeem Khan ◽  
Irum ◽  
Saba Gul ◽  
Muslima ◽  
Muhammad Mursaleen
Keyword(s):  
Human Plasma ◽  
Limit Of Detection ◽  
Sodium Dodecyl ◽  
Pharmaceutical Preparations ◽  
Pharmaceutical Products ◽  
Pure Form ◽  
Fluorescence Signal ◽  
Spectrofluorimetric Method ◽  
Relative Standard

Abstract A rapid, simple and economical spectrofluorimetric method for the determination of diclofenac potassium in pure form, in pharmaceutical preparations and in human plasma has been developed. The method is based on the enhancement of the fluorescence signal of diclofenac potassium by the addition of sodium dodecyl sulphate in McIvaine buffer with a pH of 5. Different experimental conditions such as buffer type, pH, type and concentration of surfactants were investigated. The fluorescence intensity of the solution was recorded at 361 nm after excitation at 243 nm. The method shows linearity in the concentration range of 0.2 μg mL–1–10 μg mL–1 with a good correlation coefficient of 0.997. The relative standard deviation value was 3.62 (n = 7). The limit of detection and limit of quantification were calculated to be 2.84 × 10–3 μg mL–1 and 9.47 × 10–3 μg mL-1, respectively. The effect of excipients and co-administrated drugs was investigated and no interference was observed. The method was successfully applied for the determination of diclofenac potassium in pure form, in pharmaceutical products and in human plasma. The percentage recoveries obtained ranged from 100.25% to 102.16% for pure form and 97.50% to 102.00% for pharmaceutical products and from 98.50% to 101.67% for human plasma.

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