Detection of Pseudomonas aeruginosa carried a new array of gene cassettes within class 1 integron isolated from a teaching hospital in Nanjing, China

2008 ◽  
Vol 46 (6) ◽  
pp. 687-691 ◽  
Author(s):  
Yuan Wu ◽  
Hui Li ◽  
Jun Li ◽  
Zu Hu Huang
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Teaching Hospital ◽  
Class 1 Integron ◽  
Gene Cassettes ◽  
Class 1

scholarly journals BEL-1, a Novel Clavulanic Acid-Inhibited Extended-Spectrum β-Lactamase, and the Class 1 Integron In120 in Pseudomonas aeruginosa

2005 ◽  
Vol 49 (9) ◽  
pp. 3743-3748 ◽  
Author(s):  
Laurent Poirel ◽  
Laura Brinas ◽  
Annemie Verlinde ◽  
Louis Ide ◽  
Patrice Nordmann
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Clavulanic Acid ◽  
Cloning And Sequencing ◽  
Class 1 Integron ◽  
Gene Cassettes ◽  
Esbl Gene ◽  
Class A ◽  
Class 1 ◽  
Synergy Test ◽  
Specific Sequences

ABSTRACT Screening by a double-disk synergy test identified a Pseudomonas aeruginosa isolate that produced a clavulanic acid-inhibited expanded-spectrum β-lactamase (ESBL). Cloning and sequencing identified a novel ESBL, BEL-1, weakly related to other Ambler class A ESBLs. β-Lactamase BEL-1 hydrolyzed significantly most expanded-spectrum cephalosporins and aztreonam, and its activity was inhibited by clavulanic acid, tazobactam, cefoxitin, moxalactam, and imipenem. This chromosome-encoded ESBL gene was embedded in a class 1 integron containing three other gene cassettes. In addition, this integron was bracketed by Tn1404 transposon sequences at its right end and by P. aeruginosa-specific sequences at its left end.

scholarly journals Class 1 Integron Containing a New Gene Cassette, aadA10, Associated with Tn1404 from R151

2002 ◽  
Vol 46 (8) ◽  
pp. 2400-2408 ◽  
Author(s):  
Sally R. Partridge ◽  
Christina M. Collis ◽  
Ruth M. Hall
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Gene Cassette ◽  
Class 1 Integron ◽  
Gene Cassettes ◽  
Base Element ◽  
Class 1 Integrons ◽  
Resistance Determinants ◽  
Class 1 ◽  
New Gene

ABSTRACT The carbenicillin, gentamicin, kanamycin, streptomycin, spectinomycin, sulfonamide, and tobramycin resistance determinants found on Pseudomonas aeruginosa plasmid R151 have previously been shown to translocate to another plasmid, R388, and it was inferred that a transposon, Tn1404, carried the resistance determinants. Sequencing of the cassette array from the plasmid known as R388::Tn1404 revealed two known gene cassettes, oxa10 and aadB, and a previously unidentified cassette determining resistance to streptomycin and spectinomycin, here designated aadA10, in the order oxa10-aadB-aadA10. These cassettes replaced the dfrB2-orfA cassette array of R388, indicating that movement of the resistance determinants from R151 to R388 resulted from recombinational exchange between two class 1 integrons rather than transposition. The AadA10 protein is most closely related to AadA6 (85% identical) and AadA7 (80% identical). The aadA10 cassette found here has only a simple site containing a 7-bp spacer derived from attI1 in place of a 59-base element and is likely to represent a derivative of the complete cassette. IntI1-mediated deletion of the aadA10 cassette was not detected, indicating that this single simple site is either inactive or only weakly active.

scholarly journals Molecular Characterization of a Novel Class 1 Integron Containing blaGES-1 and a Fused Product of aac(3)-Ib/aac(6")-Ib" Gene Cassettes in Pseudomonas aeruginosa

2002 ◽  
Vol 46 (3) ◽  
pp. 638-645 ◽  
Author(s):  
Véronique Dubois ◽  
Laurent Poirel ◽  
Caroline Marie ◽  
Corinne Arpin ◽  
Patrice Nordmann ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Diffusion Method ◽  
Fusion Product ◽  
Resistance Pattern ◽  
Clinical Strain ◽  
Homologous Sequence ◽  
Class 1 Integron ◽  
Gene Cassettes ◽  
Extended Spectrum ◽  
Class 1

ABSTRACT As seen by the disk diffusion method, the clinical strain of Pseudomonas aeruginosa Pa695, resistant to all extended-spectrum cephalosporins and aminoglycosides, exhibited an unusual synergistic effect between ceftazidime and imipenem. This isolate produced an extended-spectrum β-lactamase (ESBL) with a pI of 5.8 that appeared to be chromosomally encoded. Cloning experiments revealed that this ESBL was encoded by bla GES-1, previously described in an integron from Klebsiella pneumoniae. In P. aeruginosa Pa695, a higher level of resistance to ceftazidime than to ticarcillin was observed, and no synergy between the β-lactamase inhibitors and extended-spectrum cephalosporins was detected, in contrast to the resistance pattern observed in K. pneumoniae. Further sequence analysis demonstrated that the bla GES-1 gene cassette was located in a class 1 integron, which contained another sequence corresponding to the fused aac(3)-Ib and aac(6")-Ib" gene cassettes. The fusion product was functional, as was the product of each gene cloned separately: AAC(3)-I, despite the deletion of the four last amino acids, and AAC(6"), which carried three amino acid changes compared with the most homologous sequence. The AAC(3)-I protein conferred an expected gentamicin and fortimicin resistance, and the AAC(6"), despite the Leu-119→Ser substitution, yielded resistance to kanamycin, tobramycin, and dibekacin, but slightly affected netilmicin and amikacin, and had no apparent effect on gentamicin. The fusion product conveyed a large profile of resistance, combining the AAC(6") activity with a higher level of gentamicin resistance without accompanying fortimicin resistance.

scholarly journals Characterization and Movement of the Class 1 Integron Known as Tn2521 and Tn1405

2002 ◽  
Vol 46 (5) ◽  
pp. 1288-1294 ◽  
Author(s):  
Sally R. Partridge ◽  
Heidi J. Brown ◽  
Ruth M. Hall
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Homologous Recombination ◽  
Complete Sequence ◽  
Class 1 Integron ◽  
Gene Cassettes ◽  
Class 1 Integrons ◽  
Single Location ◽  
Class 1 ◽  
Backbone Structure

ABSTRACT Two putative transposons, Tn2521 and Tn1405, carrying determinants for the PSE-4 β-lactamase and for resistance to streptomycin, spectinomycin, and sulfonamides were previously isolated from the chromosome of Pseudomonas aeruginosa Dalgleish. Detailed mapping and determination of the complete sequence of Tn2521 revealed that it is a class 1 integron, here renamed In33, with a backbone structure identical to that of In4 from Tn1696. In33 contains two gene cassettes, blaP1 and aadA1, replacing the aacC1-orfE-aadA2-cmlA1 cassette array in In4. Although In33 does not include any transposition genes, movement of In33 (Tn2521) targeted to a single location in the IncP-1 plasmid R18-18 has been reported previously (M. I. Sinclair and B. W. Holloway, J. Bacteriol. 151:569-579, 1982). A 5-bp duplication of the target, which lies within the res site recognized by the ParA resolvase of R18-18, was present, indicating that the mechanism of movement was transposition. Together, these data indicate that class 1 integrons that are defective in self-transposition can move under appropriate circumstances. The Tn1405 isolate studied was found to represent only the cassette array of In33, which had replaced the cassette array in the recipient plasmid R388, probably by homologous recombination.

scholarly journals Characterization of Class 1 Integrons from Pseudomonas aeruginosa That Contain the blaVIM-2Carbapenem-Hydrolyzing β-Lactamase Gene and of Two Novel Aminoglycoside Resistance Gene Cassettes

2001 ◽  
Vol 45 (2) ◽  
pp. 546-552 ◽  
Author(s):  
Laurent Poirel ◽  
Thierry Lambert ◽  
Salih Türkoglü ◽  
Esthel Ronco ◽  
Jean-Louis Gaillard ◽  
...  
Keyword(s):  
Amino Acids ◽  
Pseudomonas Aeruginosa ◽  
Amino Acid ◽  
Resistance Gene ◽  
Gene Cassette ◽  
Amino Acid Sequences ◽  
Aminoglycoside Resistance ◽  
Class 1 Integron ◽  
Gene Cassettes ◽  
Class 1

ABSTRACT Two clonally unrelated Pseudomonas aeruginosa clinical strains, RON-1 and RON-2, were isolated in 1997 and 1998 from patients hospitalized in a suburb of Paris, France. Both isolates expressed the class B carbapenem-hydrolyzing β-lactamase VIM-2 previously identified in Marseilles in the French Riviera. In both isolates, thebla VIM-2 cassette was part of a class 1 integron that also encoded aminoglycoside-modifying enzymes. In one case, two novel aminoglycoside resistance gene cassettes,aacA29a and aacA29b, were located at the 5′ and 3′ end of the bla VIM-2 gene cassette, respectively. The aacA29a and aacA29b gene cassettes were fused upstream with a 101-bp part of the 5′ end of theqacE cassette. The deduced amino acid sequence AAC(6′)-29a protein shared 96% identity with AAC(6′)-29b but only 34% identity with the aacA7-encoded AAC(6′)-I1, the closest relative of the AAC(6′)-I family enzymes. These aminoglycoside acetyltransferases had amino acid sequences much shorter (131 amino acids) than the other AAC(6′)-I enzymes (144 to 153 amino acids). They conferred resistance to amikacin, isepamicin, kanamycin, and tobramycin but not to gentamicin, netilmicin, and sisomicin.

scholarly journals A Plasmidic Class 1 Integron from Five Pseudomonas aeruginosa Clinical Strains Harbored aacA4 and Nonsense-mutated cmlA1 Gene Cassettes

2007 ◽  
Vol 53 (6) ◽  
pp. 750-755 ◽  
Author(s):  
He Yan ◽  
Lei Shi ◽  
Shinji Yamasaki ◽  
Xinhui Li ◽  
Yicheng Cao ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Class 1 Integron ◽  
Gene Cassettes ◽  
Clinical Strains ◽  
Class 1

scholarly journals Class 1 Integron Containing Metallo-β-Lactamase Gene blaVIM-2 in Pseudomonas aeruginosa Clinical Strains Isolated in Japan

2004 ◽  
Vol 48 (2) ◽  
pp. 626-628 ◽  
Author(s):  
Jun Yatsuyanagi ◽  
Shioko Saito ◽  
Seizaburo Harata ◽  
Noriyuki Suzuki ◽  
Yuko Ito ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Class 1 Integron ◽  
Gene Cassettes ◽  
Clinical Strains ◽  
Class 1 ◽  
Horizontal Spread ◽  
Lactamase Gene

ABSTRACT Four bla VIM-2 gene-harboring Pseudomonas aeruginosa strains were identified. These strains possessed a class 1 integron harboring ORF1, bla VIM-2, and aacA4 gene cassettes. The transposon-mediated horizontal spread of the bla VIM-2 gene among these strains was suggested, which increases the threat that the bla VIM-2 gene will disseminate among diverse genera of bacteria.

Sign in / Sign up

Export Citation Format

Share Document