Molecular cloning and expression analysis of a delta 6-fatty acid desaturase gene from Rhizopus stolonifer strain YF6 which can accumulate high levels of gamma-linolenic acid

2011 ◽  
Vol 49 (1) ◽  
pp. 151-154 ◽  
Author(s):  
Xia Wan ◽  
Yinbo Zhang ◽  
Ping Wang ◽  
Mulan Jiang
Keyword(s):  
Fatty Acid ◽  
Molecular Cloning ◽  
Linolenic Acid ◽  
Expression Analysis ◽  
Fatty Acid Desaturase ◽  
Cloning And Expression ◽  
Rhizopus Stolonifer ◽  
Gamma Linolenic Acid ◽  
Desaturase Gene ◽  
Fatty Acid Desaturase Gene

Saccharomyces kluyveri FAD3 encodes an ω3 fatty acid desaturase

Microbiology ◽  
2004 ◽  
Vol 150 (6) ◽  
pp. 1983-1990 ◽  
Author(s):  
Takahiro Oura ◽  
Susumu Kajiwara
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Linoleic Acid ◽  
Linolenic Acid ◽  
Functional Characterization ◽  
Fatty Acid Desaturase ◽  
Fatty Acid Desaturases ◽  
Desaturase Gene ◽  
Saccharomyces Kluyveri ◽  
Fatty Acid Desaturase Gene

Fungi, like plants, are capable of producing the 18-carbon polyunsaturated fatty acids linoleic acid and α-linolenic acid. These fatty acids are synthesized by catalytic reactions of Δ12 and ω3 fatty acid desaturases. This paper describes the first cloning and functional characterization of a yeast ω3 fatty acid desaturase gene. The deduced protein encoded by the Saccharomyces kluyveri FAD3 gene (Sk-FAD3) consists of 419 amino acids, and shows 30–60 % identity with Δ12 fatty acid desaturases of several eukaryotic organisms and 29–31 % identity with ω3 fatty acid desaturases of animals and plants. During Sk-FAD3 expression in Saccharomyces cerevisiae, α-linolenic acid accumulated only when linoleic acid was added to the culture medium. The disruption of Sk-FAD3 led to the disappearance of α-linolenic acid in S. kluyveri. These findings suggest that Sk-FAD3 is the only ω3 fatty acid desaturase gene in this yeast. Furthermore, transcriptional expression of Sk-FAD3 appears to be regulated by low-temperature stress in a manner different from the other fatty acid desaturase genes in S. kluyveri.

Molecular Cloning, Characterization, and Expression of an ω-3 Fatty Acid Desaturase Gene from Sapium sebiferum

2008 ◽  
Vol 106 (4) ◽  
pp. 375-380 ◽  
Author(s):  
Bei Niu ◽  
Liang Guo ◽  
Mingming Zhao ◽  
Tong Luo ◽  
Rong Zhang ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Molecular Cloning ◽  
Fatty Acid Desaturase ◽  
Sapium Sebiferum ◽  
Desaturase Gene ◽  
Fatty Acid Desaturase Gene
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