Cloning and expression of the Δ 9 fatty acid desaturase gene from Cryptococcus curvatus ATCC 20509 containing histidine boxes and a cytochrome b 5 domain

1997 ◽  
Vol 47 (6) ◽  
pp. 663-667 ◽  
Author(s):  
P. A. E. P. Meesters ◽  
J. Springer ◽  
G. Eggink
2000 ◽  
Vol 24 (6) ◽  
pp. 805-813 ◽  
Author(s):  
Gorou Horiguchi ◽  
Takuichi Fuse ◽  
Naoto Kawakami ◽  
Hiroaki Kodama ◽  
Koh Iba

2010 ◽  
Vol 87 (1) ◽  
pp. 215-224 ◽  
Author(s):  
Takahito Watanabe ◽  
Saeko Tsuda ◽  
Hiroshi Nishimura ◽  
Yoichi Honda ◽  
Takashi Watanabe

2008 ◽  
Vol 17 (4) ◽  
pp. 717-725 ◽  
Author(s):  
Guiming Zhu ◽  
Hongxing Chen ◽  
Xiaojie Wu ◽  
Yanrong Zhou ◽  
Jianshen Lu ◽  
...  

Microbiology ◽  
2004 ◽  
Vol 150 (6) ◽  
pp. 1983-1990 ◽  
Author(s):  
Takahiro Oura ◽  
Susumu Kajiwara

Fungi, like plants, are capable of producing the 18-carbon polyunsaturated fatty acids linoleic acid and α-linolenic acid. These fatty acids are synthesized by catalytic reactions of Δ12 and ω3 fatty acid desaturases. This paper describes the first cloning and functional characterization of a yeast ω3 fatty acid desaturase gene. The deduced protein encoded by the Saccharomyces kluyveri FAD3 gene (Sk-FAD3) consists of 419 amino acids, and shows 30–60 % identity with Δ12 fatty acid desaturases of several eukaryotic organisms and 29–31 % identity with ω3 fatty acid desaturases of animals and plants. During Sk-FAD3 expression in Saccharomyces cerevisiae, α-linolenic acid accumulated only when linoleic acid was added to the culture medium. The disruption of Sk-FAD3 led to the disappearance of α-linolenic acid in S. kluyveri. These findings suggest that Sk-FAD3 is the only ω3 fatty acid desaturase gene in this yeast. Furthermore, transcriptional expression of Sk-FAD3 appears to be regulated by low-temperature stress in a manner different from the other fatty acid desaturase genes in S. kluyveri.


2018 ◽  
Vol 7 (1) ◽  
pp. 98 ◽  
Author(s):  
Vahid Mansouri ◽  
Shaghayegh Haghjooy Javanmard ◽  
Manijeh Mahdavi ◽  
MohammadHasan Tajedini

PLoS ONE ◽  
2018 ◽  
Vol 13 (1) ◽  
pp. e0191432 ◽  
Author(s):  
Yufei Xue ◽  
Baojun Chen ◽  
Aung Naing Win ◽  
Chun Fu ◽  
Jianping Lian ◽  
...  

FEBS Letters ◽  
2004 ◽  
Vol 573 (1-3) ◽  
pp. 45-50 ◽  
Author(s):  
Dongsheng Wei ◽  
Mingchun Li ◽  
Xinxin Zhang ◽  
Yong Ren ◽  
Laijun Xing

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