Optimization of Enterobacter cloacae (KU923381) for diesel oil degradation using response surface methodology (RSM)

2017 ◽  
Vol 55 (2) ◽  
pp. 104-111 ◽  
Author(s):  
Sugumar Ramasamy ◽  
Arumugam Arumugam ◽  
Preethy Chandran
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Enterobacter Cloacae ◽  
Diesel Oil ◽  
Oil Degradation ◽  
Diesel Oil Degradation

scholarly journals Kinetics and Optimization by Response Surface Methodology of Aerobic Bioremediation. Geoelectrical Parameter Monitoring

2020 ◽  
Vol 10 (1) ◽  
pp. 405 ◽  
Author(s):  
Carla Maria Raffa ◽  
Fulvia Chiampo ◽  
Alberto Godio ◽  
Andrea Vergnano ◽  
Francesca Bosco ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Dielectric Permittivity ◽  
Response Surface ◽  
Reaction Rates ◽  
Diesel Oil ◽  
Second Order ◽  
Coaxial Cable ◽  
Oil Concentration ◽  
Two Parameters ◽  
Aerobic Bioremediation

This study aimed to investigate the kinetics of an aerobic bioremediation process of diesel oil removal by indigenous microorganisms, and to define the optimal operative conditions by means of response surface methodology. This was carried out by setting up a series of microcosms (200 g of soil), polluted with the same diesel oil concentration (70 g·kg−1 of soil), but with different water contents (u%) and carbon to nitrogen (C/N) ratios. The process was monitored by: (1) residual diesel oil concentration, to measure the removal efficiency, and (2) fluorescein production, to check the microbial activity. These two parameters were the objective variables used for the analysis of variance (ANOVA) and response surface methodology (RSM). The results allowed the interactions between u% and C/N to be defined and the optimal range to be adopted for each. The process kinetics was modeled with first- and second-order reaction rates; slightly better results were achieved for the second-order model in terms of parameter variability. Biological processes like degradation may have effects on dielectric properties of soil; an open-ended coaxial cable was used to measure the dielectric permittivity of microcosm matrices at the start and after 130 days of bioremediation. The evolution of the real and the imaginary components of dielectric permittivity provided results that supported the evidence of a biodegradation process in progress.

scholarly journals Est16, a New Esterase Isolated from a Metagenomic Library of a Microbial Consortium Specializing in Diesel Oil Degradation

PLoS ONE ◽  
2015 ◽  
Vol 10 (7) ◽  
pp. e0133723 ◽  
Author(s):  
Mariana Rangel Pereira ◽  
Gustavo Fernando Mercaldi ◽  
Thaís Carvalho Maester ◽  
Andrea Balan ◽  
Eliana Gertrudes de Macedo Lemos
Keyword(s):  
Microbial Consortium ◽  
Metagenomic Library ◽  
Diesel Oil ◽  
Oil Degradation ◽  
Diesel Oil Degradation

Isolation and Characterization of Petroleum Hydrocarbon Degrading Bacteria from the Bohai Sea, China

2014 ◽  
Vol 955-959 ◽  
pp. 728-731
Author(s):  
Ping Guo ◽  
Jian Guo Lin ◽  
Bin Xia Cao ◽  
Na Ta
Keyword(s):  
Petroleum Hydrocarbon ◽  
Bohai Sea ◽  
Diesel Oil ◽  
Contaminated Site ◽  
Oil Degradation ◽  
Isolation And Characterization ◽  
Degrading Bacteria ◽  
Luxuriant Growth ◽  
Diesel Oil Degradation

Fourteen petroleum hydrocarbon degrading bacteria strains were isolated from oil-contaminated site. Isolated strains were able use diesel oil as sole carbon and energy source. Bacterial strain HD1 was selected due to the luxuriant growth on oil agar. The oil degradation rate of strain HD1 was analyzed using UV-spectrometry-based methods. The result showed that the rate of diesel oil degradation of 75% was observed after 14days of cultivation.

scholarly journals Enhancement diesel oil degradation by using biofilm forming bacteria on biochar

2018 ◽  
Vol 9 (1) ◽  
pp. 26-31
Author(s):  
Thi Nhi Cong Le ◽  
Thi Ngoc Mai Cung ◽  
Ngoc Huy Vu ◽  
Thi Lien Do ◽  
Thi To Uyen Do ◽  
...  
Keyword(s):  
Climate Change Mitigation ◽  
Crop Residues ◽  
Diesel Oil ◽  
Oil Degradation ◽  
Pseudomonas Sp ◽  
Wood Biomass ◽  
Porous Substance ◽  
Fine Grained ◽  
Change Mitigation ◽  
Diesel Oil Degradation

Biochar is defined as a carbon-rich, fine-grained, porous substance, which is produced by pyrolysis biomass with little or no oxygen. Biochar is usually produced from crop residues, wood biomass, animal litters, and solid wastes. Recently, biochar is increasingly receiving attention as an environmental-friendly approach, especially as a climate change mitigation strategy. Biochar is especilly demonstrated to remove diesel oil (DO) from soil and water. In this report, 4 biofilm forming bacteria including Klepsiellasp. VTD8, Pseudomonas sp. BQN21, Rhodococcussp. BN5 and Stenotropomonassp. QND8 were used to attach to biochar produced from husk to estimate the capacity of their DO removal. As the results, removal efficiency of biofilm formed by each strain VTD8, BQN21, BN5 and QND8 were 67, 73, 75 and 68 % with initial concentration of 39 g/l, respectively. On the other hand, mix species biofilm attached to husk carrier and without carrier degraded 98 and 78 %. Using husk without bacteria as absortion control, the amount of DO removal was 23 %. These results gave hint that using biochar produced from husk as carrier for biofilm forming bacteria to attach may increase efficiency of DO pollution treatment. Than sinh học (biochar) là một chất xốp có các gốc carbon và có nguồn gốc từ quá trình nhiệt phân sinh khối các loại chất thải, động, thực vật,… dưới điều kiện hạn chế oxy hoặc không có oxy.Hiện nay biochar đã được ứng dụng rộng rãi trong xử lý môi trường. Đặc biệt các biochar còn được chứng minh là có thể xử lý dầu diesel (diesel oil - DO) có trong đất và nước. Trong nghiên cứu này, chúng tôi sử dụng 4 chủng vi khuẩn tạo màng sinh học tốt là Klepsiella sp. VTD8, Pseudomonas sp. BQN21, Rhodococcus sp. BN5 và Stenotropomonas sp. QND8 để gắn lên chất mang là biochar làm từ trấu nhằm đánh giá hiệu quả xử lý DO của chúng. Kết quả cho thấy, sau 7 ngày, các chủng VTD8, BQN21, BN5 và QND8 có khả năng phân hủy 67, 73, 75 và 68 % DO với hàm lượng ban đầu là 39 g/l. Trong khi đó, hiệu suất của màng sinh học tạo thành bởi hỗn hợp các chủng này khi không có chất mang biochar trấu và khi có chất mang biochar trấu lần lượt là 78 và 98 %. Còn sử dụng chất mang biochar trấu không có vi sinh vật làm đối chứng thì thu được hiệu suất hấp phụ DO là 23 %. Như vậy, kết quả này mở ra tiềm năng ứng dụng biochar trấu làm chất mang cho các chủng vi khuẩn tạo màng sinh học để nâng cao hiệu quả xử lý ô nhiễm dầu.

Isolation of Petroleum Hydrocarbon Degrading Bacteria from the Liaohe Estuary at Cold Climate

2015 ◽  
Vol 1092-1093 ◽  
pp. 878-881
Author(s):  
Ping Guo ◽  
Jian Guo Lin ◽  
Bin Xia Cao ◽  
Na Ta
Keyword(s):  
Contaminated Soil ◽  
Petroleum Hydrocarbon ◽  
Degradation Rate ◽  
Diesel Oil ◽  
Cold Climate ◽  
Hydrocarbon Degradation ◽  
Oil Degradation ◽  
Degrading Bacteria ◽  
Cold Tolerant ◽  
Diesel Oil Degradation

Two cold-tolerant petroleum hydrocarbon degrading bacteria strain named CHD1 and CHD2 were isolated from oil-contaminated soil at cold climate. The isolated strains were able use diesel oil as sole carbon. The petroleum hydrocarbon degradation rate was analyzed using UV-spectrometry-based methods. The results showed that the diesel oil degradation rate of CHD1 and CHD2 were 22% and 25%, respectively.

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