Influence of the Zinc and Fibre Addition in the Diet on Biomechanical Bone Properties in Weaned Piglets

The effects of the zinc and fibre source in piglets’ diet on the bone mineral content, density, and strength parameters of the femur were investigated using 24 piglets fed a diet supplemented with either lignocellulose (LC) or potato fibre (PF). Half of each group of piglets consumed a diet with ZnSO4 monohydrate or with zinc glycinate (ZnGly). The diets contained similar amounts of lysine, energy, and fibre. Bone mineral content and density were over 9% higher in pigs receiving diets with ZnGly than in animals fed diets with ZnSO4. Moreover, ZnGly strongly improved maximum and elastic strength (by 25.7 and 20.0%, respectively, p < 0.0001) and bone stiffness (by 29.4%, p < 0.0001). Only the mass of the femur was affected by the type of fibre in the diet, as the femurs of piglets fed diets with LC were over 7% (p < 0.0001) heavier than in piglets fed diets with PF. The intake of digestible zinc and the zinc content in the blood serum were positively correlated with the measured bone parameters and, depending on the parameter, “r” ranged from 0.749 to 0.866 and from 0.400 to 0.479, respectively. It can be concluded that bone parameters are affected more strongly by the organic than inorganic source of zinc.

Geology and pyrite-polymetallic mineralization of the forecasted Limonite deposit, Rassokhinsky ore cluster (Yenisei ridge)

The development of Russia’s largest Gorevskoye leadzinc deposit (Gorevsky GOK, Novoangarsky OK) will depend on promising deposits of the Angara polymetallic ore region, in particular, those of the Rassokhinsky ore cluster. Based on the analysis of geological and geophysical information of previous and current studies, a major Limonite pyrite-polymetallic (massive sulfide) deposit is forecasted within it. The main lithological and stratigraphic criterion for the localization of ores is determined by their confinement to high-carbon black quartz-sericite schists of the upper subformation of the Potoskuy suite of the Upper Riphean. In the top of this pack, layered and massive pyrite-polymetallic ores, 70– 140 m thick, are localized. Based on PPA data, lead and zinc content in core varies from fractions of a percent to several percent, based on the ICP-AES method: 0,1– 3,7 % Pb, 0,1–6,4 % Zn, Pb : Zn ratio is 1 : (1–10).

Stage specific comparative transcriptomic analysis to reveal gene networks regulating iron and zinc content in pearl millet [Pennisetum glaucum (L.) R. Br.]

Pearl millet is an important staple food crop of poor people and excels all other cereals due to its unique features of resilience to adverse climatic conditions. It is rich in micronutrients like iron and zinc and amenable for focused breeding for these micronutrients along with high yield. Hence, this is a key to alleviate malnutrition and ensure nutritional security. This study was conducted to identify and validate candidate genes governing grain iron and zinc content enabling the desired modifications in the genotypes. Transcriptome sequencing using ION S5 Next Generation Sequencer generated 43.5 million sequence reads resulting in 83,721 transcripts with N50 of 597 bp and 84.35% of transcripts matched with the pearl millet genome assembly. The genotypes having high iron and zinc showed differential gene expression during different stages. Of which, 155 were up-regulated and 251 were down-regulated while during flowering stage and milking stage 349 and 378 transcripts were differentially expressed, respectively. Gene annotation and GO term showed the presence of transcripts involved in metabolic activities associated with uptake and transport of iron and zinc. Information generated will help in gaining insights into iron and zinc metabolism and develop genotypes with high yield, grain iron and zinc content.

Lack of ZnT8 protects pancreatic islets from hypoxia- and cytokine induced cell death

Pancreatic β-cells depend on the well-balanced regulation of cytosolic zinc concentrations, providing sufficient zinc ions for the processing and storage of insulin, but avoiding toxic effects. The zinc transporter ZnT8, encoded by SLC30A8, is a key player regarding islet cell zinc homeostasis, and polymorphisms in this gene are associated with altered type 2 diabetes susceptibility in man. The objective of this study was to investigate the role of ZnT8 and zinc in situations of cellular stress as hypoxia or inflammation. Isolated islets of wild-type and global ZnT8-/- mice were exposed to hypoxia or cytokines and cell death was measured. To explore the role of changing intracellular Zn2+ concentrations, wild-type islets were exposed to different zinc concentrations using zinc chloride or the zinc chelator N,N,N′,N′-tetrakis(2-pyridinylmethyl)-1,2-ethanediamine (TPEN). Hypoxia or cytokine (TNFα, IFNγ, IL1β) treatment induced islet cell death, but to a lesser extent in islets from ZnT8-/- mice, which were shown to have a reduced zinc content. Similarly, chelation of zinc with TPEN reduced cell death in wild-type islets treated with hypoxia or cytokines, whereas increased zinc concentrations aggravated the effects of these stressors. This study demonstrates a reduced rate of cell death in islets from ZnT8-/- mice as compared to wild-type islets when exposed to two distinct cellular stressors, hypoxia or cytotoxic cytokines. This protection from cell death is, in part, mediated by a reduced zinc content in islet cells of ZnT8-/- mice. These findings may be relevant for altered diabetes burden in carriers of risk SLC30A8 alleles in man.

A Compatible and Efficient Fabrication of Bi-Continuous Nanoporous Copper Films

To cater to the application in various micro/nano devices, this paper reports a compatible and efficient approach combining electrodeposition and dealloying to fabricate bi-continuous nanoporous copper films. In the electrodeposition step, effect of concentration ratio of different ions, pH of the solution and cathode current density on the elementary composition and microscopic morphology of the deposited alloy are systematically investigated, obtaining an optimum condition with good stability and process compatibility. A uniform copper-zinc alloy with its zinc content improved to ~67 at.% is prepared under this condition. A uniform nanoporous copper with an average pore size of 150 nm is fabricated by ultrasonic assisted dealloying whose efficiency is significantly improved compared to free dealloying. This method is promising to be used in the mass production of nanoporous copper films, benefiting researches on various practical applications in micro/nano devices.

Technology Elaboration of the Semi-finished Dough Product “Amygdalaceous” for Cheesecakes

Topicality. In this paper common nutritional status disorders of the present are analysed. Additionally, it is determined that one of the leading places among them is given to the zinc deficiency. The probable causes of zinc deficiency are offered, and the works of scientists dealing with this problem are analysed. It is defined that the development of zinc enriched technologies in desserts production for restaurant industry establishments, as well as semi-finished products for their yielding, is of urgent importance. Aim and methods. The aim of this study is to substantiate and elaborate the technology of the semi-finished dough product “Amygdalaceous” for cheesecakes, which should be characterized by a raised zinc content. To achieve the set aim, empirical, organoleptic, mathematical, statistical and computational research methods are used. Results. Recipes of the semi-finished dough product model compositions are substantiated and created, their physico-chemical parameters and organoleptic characteristics are studied. The technology of the semi-finished dough product “Amygdalaceous” for cheesecakes is elaborated, the technological scheme of its production is offered. The chemical content and energy value of the elaborated semi-finished product are analysed, as well as its integral score is calculated. Conclusions and discussion. It is determined that the model composition is characterised by the best indicators, which provides adding in the recipe the almond flour in amount 14.5 % mass. It is revealed that the elaborated product is characterised by the raised content of proteins, fats, zinc, potassium, calcium, magnesium and phosphorus, and the decreased content of carbohydrates. It is proved that the consumption of the elaborated semi-finished product can provide the daily requirement for zinc by 31,87 %, which corresponds to the mentioned above aim of the study. The scientific novelty of the obtained results lies in the development of technologies elaboration principles for meals, culinary and semi-finished products with a raised zinc content. The practical significance of the obtained results can be seen in the expansion of semi-finished products and desserts assortment for restaurant industry establishments, and in assistance of the Ukrainian nation enhancement.

Undervalued Spiny Monkey Orange (Strychnos spinosa Lam.): An Indigenous Fruit for Sustainable Food-Nutrition and Economic Prosperity

Strychnos spinosa Lam. is among the top nutrient-dense indigenous fruit species that are predominant in Southern Africa. It is a highly ranked indigenous fruit based on the nutrition and sensorial properties, which make it an important food source for the marginalized rural people. On the basis of the high vitamin C, iron, and zinc content, it has the capacity to improve the food- nutrition and the socioeconomic status of individuals, especially those in the rural areas of the developing nations. The nutritional composition of Strychnos spinosa compare favorably with many of the popular fruits, such as strawberries and orange. Additionally, Strychnos spinosa has antioxidant activity similar to well-known antioxidant fruits, which keeps it in the class of the popular fruits, giving it added nutrition–health-promoting benefits. In order to improve the availability of Strychnos spinosa, more research on the domestication, processing, preservation, value chain, and economic potential need to be further explored. Therefore, we recommend more concerted efforts from relevant stakeholders with interest in Strychnos spinosa fruit production as a possible sustainable solution to food shortage, food-nutrition insecurity, malnutrition, and austerity, mainly in the rural communities of the developing countries.

Biochemical Analysis Based on Zinc Uptake of Chickpea (Cicer arietinum L.) Varieties Infected by Meloidogyne incognita

The significant constraints in Chickpea (Cicer arietinum L.) production hampers a bit more than 14% global yield loss due to plant-parasitic nematodes. Root-knot nematode (Meloidogyne sp.) is an endoparasite and a significant species affecting the chickpea plant. So, the chemical basis of management is more cost-effective, and pest resurgence building is enhanced in the pathogen. So, ecological-based nematode management is requisite, which also is got hampered due to breeding for resistance against such plant-parasitic nematodes. This was the primary reason to conduct this experiment to enhance resistance in the chickpea plants based on Zinc uptake by using bioagent, Pseudomonas fluorescens alone or in combination. where Different treatments including nematode, bacterium, and chemicals were used sustaining the enhancement of disease resistance in chickpea cultivars, RSG 974, GG 5, GNG 2144. Zinc content of chickpea variety GNG 2144 was found the highest in treatment, when only bacterium (P. fluorescens) was inoculated, i.e., 3.14 mg/100g of root followed by GG 5, i.e., 2.79 mg/100g of root and RSG 974 was, i.e., 2.35 mg/100g of root respectively in a descending order. Application of P. fluorescence combined or alone gradually increased the Zn concentration in roots of chickpea plants compared to healthy check followed by chemical treated plants.

Effect of Zinc Fortification on Quality, Yield and Economics of Sweetcorn

A field experiment was performed at Agricultural College, Bapatla, ANGRAU, Guntur during kharif season of 2020 to evaluate the efficacy of zinc nutrition on quality, yield and economics of sweetcorn. Experiment was carried out in Randomized Block Design and comprising of nine treatments with three replications. The results disclosed that application of recommended dose of fertilizers along with soil application of 10 kg ha-1 Zn EDTA + two foliar sprays of nano zinc @ 250 ppm at 20 & 40 DAS registered remarkably higher protein content (12.98%) and zinc content (34.59 ppm) in kernel which was considered to be superior over the remaining zinc management practices tried. Also, highest green cob (12,638 kg ha-1), green fodder (19,674 kg ha-1), stover yield (7,590 kg ha-1) and gross returns (₹ 1,93,360 ha-1) was recorded from the treatment with soil application of Zn EDTA @ 10 kg ha-1 + two foliar sprays of nano zinc @ 250 ppm at 20 & 40 DAS along with RDF over control. However, highest net returns (₹ 1,38,664 ha-1) and benefit cost ratio (2.74) was recorded with RDF + ZnSO4 @ 25 kg ha-1 (Soil) + Nano ZnO @ 250 ppm at 20 and 40 DAS (Foliar spray). The effectiveness of Zn EDTA compared to ZnSO4 is responsible for the higher quality parameters and yield of sweetcorn but due to its higher cost, Net returns and B;C ratio are maximum for the plots treated with ZnSO4 making it more economically viable.