scholarly journals In vitro selection and characterization of polyethylene glycol (PEG) tolerant callus lines and regeneration of plantlets from the selected callus lines in sugarcane (Saccharum officinarum L.)

2013 ◽  
Vol 19 (2) ◽  
pp. 261-268 ◽  
Author(s):  
Srinath Rao ◽  
Jabeen FTZ
2014 ◽  
Vol 50 (6) ◽  
pp. 766-776 ◽  
Author(s):  
Ashok A. Nikam ◽  
Rachayya M. Devarumath ◽  
Mahadeo G. Shitole ◽  
Vikram S. Ghole ◽  
Prahlad N. Tawar ◽  
...  

1998 ◽  
Vol 20 (3) ◽  
pp. 269-275 ◽  
Author(s):  
Gyana Ranjan Rout ◽  
Sanghamitra Samantaray ◽  
Premananda Das

2017 ◽  
Vol 1 (1) ◽  
pp. 74-84
Author(s):  
Ahmad Riduan ◽  
Rainiyati Rainiyati ◽  
Yulia Alia

Every plant rhizospheres in any ecosystem there are various living microorganisms including Arbuscular Mycorrhizae Fungi (AMF).  An isolation and characterization is required to investigate the species or type of the AMF. This research was aimed at studying the isolation and characterization of AMF sporulation in soybean rhizospheres in Jambi Province. The results of evaluation on soil samples before trapping showed that there are spores from three genus of AMF twelve types Glomus , two types Acaulospora and one type of Enthrophospora.  Following single spore culture in soybean rhizosphere, 5 spore types were obtained:  Glomus sp-1, Glomus sp-4, Glomus sp-7, Glomus sp-8 Glomus sp-10.


2003 ◽  
Vol 77 (6) ◽  
pp. 3669-3679 ◽  
Author(s):  
Caterina Trozzi ◽  
Linda Bartholomew ◽  
Alessandra Ceccacci ◽  
Gabriella Biasiol ◽  
Laura Pacini ◽  
...  

ABSTRACT The hepatitis C virus (HCV) serine protease is necessary for viral replication and represents a valid target for developing new therapies for HCV infection. Potent and selective inhibitors of this enzyme have been identified and shown to inhibit HCV replication in tissue culture. The optimization of these inhibitors for clinical development would greatly benefit from in vitro systems for the identification and the study of resistant variants. We report the use HCV subgenomic replicons to isolate and characterize mutants resistant to a protease inhibitor. Taking advantage of the replicons' ability to transduce resistance to neomycin, we selected replicons with decreased sensitivity to the inhibitor by culturing the host cells in the presence of the inhibitor and neomycin. The selected replicons replicated to the same extent as those in parental cells. Sequence analysis followed by transfection of replicons containing isolated mutations revealed that resistance was mediated by amino acid substitutions in the protease. These results were confirmed by in vitro experiments with mutant enzymes and by modeling the inhibitor in the three-dimensional structure of the protease.


2017 ◽  
Vol 9 (4) ◽  
pp. 233-268 ◽  
Author(s):  
Annamaria Ruscito ◽  
Erin M. McConnell ◽  
Anna Koudrina ◽  
Ranganathan Velu ◽  
Christopher Mattice ◽  
...  

2008 ◽  
Vol 7 (4) ◽  
pp. 641-650 ◽  
Author(s):  
J.M. Matheka ◽  
E. Magiri ◽  
A.O. Rasha ◽  
J. Machuka

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