In vitro selection and characterization of a callus line ofVigna radiata resistant to NaCl, KCl and Na2SO4

1994 ◽  
Vol 36 (1) ◽  
Author(s):  
A. Gulati ◽  
P. K. Jaiwal
Keyword(s):  
In Vitro Selection ◽  
Callus Line

PENGEMBANGAN TEKNOLOGI SELEKSI IN VITRO BERULANG (REPEAT CYCLING–IN VITRO SELECTION) TOLERAN STRES KEKERINGAN UNTUK PERBAIKAN MUTU GENETIK DAN PRODUKSI TANAMAN KEDELAI BERMIKORIZA

2017 ◽  
Vol 1 (1) ◽  
pp. 74-84
Author(s):  
Ahmad Riduan ◽  
Rainiyati Rainiyati ◽  
Yulia Alia
Keyword(s):  
Arbuscular Mycorrhizae ◽  
In Vitro Selection ◽  
Soil Samples ◽  
Single Spore ◽  
Isolation And Characterization ◽  
Single Spore Culture ◽  
Spore Culture ◽  
Glomus Sp

Every plant rhizospheres in any ecosystem there are various living microorganisms including Arbuscular Mycorrhizae Fungi (AMF).  An isolation and characterization is required to investigate the species or type of the AMF. This research was aimed at studying the isolation and characterization of AMF sporulation in soybean rhizospheres in Jambi Province. The results of evaluation on soil samples before trapping showed that there are spores from three genus of AMF twelve types Glomus , two types Acaulospora and one type of Enthrophospora.  Following single spore culture in soybean rhizosphere, 5 spore types were obtained:  Glomus sp-1, Glomus sp-4, Glomus sp-7, Glomus sp-8 Glomus sp-10.

scholarly journals In Vitro Selection and Characterization of Hepatitis C Virus Serine Protease Variants Resistant to an Active-Site Peptide Inhibitor

2003 ◽  
Vol 77 (6) ◽  
pp. 3669-3679 ◽  
Author(s):  
Caterina Trozzi ◽  
Linda Bartholomew ◽  
Alessandra Ceccacci ◽  
Gabriella Biasiol ◽  
Laura Pacini ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Serine Protease ◽  
In Vitro Selection ◽  
Three Dimensional ◽  
Host Cells ◽  
Dimensional Structure ◽  
In Vitro Systems

ABSTRACT The hepatitis C virus (HCV) serine protease is necessary for viral replication and represents a valid target for developing new therapies for HCV infection. Potent and selective inhibitors of this enzyme have been identified and shown to inhibit HCV replication in tissue culture. The optimization of these inhibitors for clinical development would greatly benefit from in vitro systems for the identification and the study of resistant variants. We report the use HCV subgenomic replicons to isolate and characterize mutants resistant to a protease inhibitor. Taking advantage of the replicons' ability to transduce resistance to neomycin, we selected replicons with decreased sensitivity to the inhibitor by culturing the host cells in the presence of the inhibitor and neomycin. The selected replicons replicated to the same extent as those in parental cells. Sequence analysis followed by transfection of replicons containing isolated mutations revealed that resistance was mediated by amino acid substitutions in the protease. These results were confirmed by in vitro experiments with mutant enzymes and by modeling the inhibitor in the three-dimensional structure of the protease.

In Vitro Selection and Characterization of DNA Aptamers to a Small Molecule Target

2017 ◽  
Vol 9 (4) ◽  
pp. 233-268 ◽  
Author(s):  
Annamaria Ruscito ◽  
Erin M. McConnell ◽  
Anna Koudrina ◽  
Ranganathan Velu ◽  
Christopher Mattice ◽  
...  
Keyword(s):  
Small Molecule ◽  
In Vitro Selection ◽  
Dna Aptamers

In vitro Selection and Characterization of Drought Tolerant Somaclones of Tropical Maize (Zea mays L.)

2008 ◽  
Vol 7 (4) ◽  
pp. 641-650 ◽  
Author(s):  
J.M. Matheka ◽  
E. Magiri ◽  
A.O. Rasha ◽  
J. Machuka
Keyword(s):  
Zea Mays ◽  
In Vitro Selection ◽  
Zea Mays L ◽  
Tropical Maize ◽  
Drought Tolerant

In vitro selection and characterization of DARPins and Fab fragments for the co-crystallization of membrane proteins: The Na+-citrate symporter CitS as an example

2007 ◽  
Vol 159 (2) ◽  
pp. 206-221 ◽  
Author(s):  
Thomas Huber ◽  
Daniel Steiner ◽  
Daniela Röthlisberger ◽  
Andreas Plückthun
Keyword(s):  
Membrane Proteins ◽  
In Vitro Selection ◽  
Fab Fragments

In vitro selection and characterization of buffelgrass somaclones with different responses to water stress

2017 ◽  
Vol 130 (2) ◽  
pp. 265-277 ◽  
Author(s):  
Edgardo Carloni ◽  
Exequiel Tommasino ◽  
Eliana López Colomba ◽  
Andrea Ribotta ◽  
Mariana Quiroga ◽  
...  
Keyword(s):  
Water Stress ◽  
In Vitro Selection

scholarly journals In vitro selection and characterization of a stable subdomain of phosphoribosylanthranilate isomerase

FEBS Journal ◽  
2005 ◽  
Vol 272 (14) ◽  
pp. 3684-3697 ◽  
Author(s):  
Wayne M. Patrick ◽  
Jonathan M. Blackburn
Keyword(s):  
In Vitro Selection

scholarly journals In vitro selection and characterization of cellulose-binding DNA aptamers

2007 ◽  
Vol 35 (19) ◽  
pp. 6378-6388 ◽  
Author(s):  
B. J. Boese ◽  
R. R. Breaker
Keyword(s):  
In Vitro Selection ◽  
Dna Aptamers ◽  
Cellulose Binding

scholarly journals Characterization of a Cytolytic Strain of Equine Infectious Anemia Virus

2003 ◽  
Vol 77 (4) ◽  
pp. 2385-2399 ◽  
Author(s):  
Wendy Maury ◽  
Patrick J. Wright ◽  
Sarahann Bradley
Keyword(s):  
In Vitro Selection ◽  
Equine Infectious Anemia Virus ◽  
Fibroblast Cell ◽  
Open Reading Frames ◽  
Fusogenic Activity ◽  
Equine Infectious Anemia ◽  
Anemia Virus ◽  
Receptor Interference

ABSTRACT A novel strain of equine infectious anemia virus (EIAV) called vMA-1c that rapidly and specifically killed infected equine fibroblasts (ED cells) but not other infectible cell lines was established. This strain was generated from an avirulent, noncytopathic strain of EIAV, MA-1. Studies with this new cytolytic strain of virus have permitted us to define viral parameters associated with EIAV-induced cell killing and begin to explore the mechanism. vMA-1c infection resulted in induction of rapid cell death, enhanced fusogenic activity, and increased rates of spread in equine fibroblasts compared to other strains of EIAV. The highly cytolytic nature of vMA-1c suggested that this strain might be superinfecting equine fibroblasts. Receptor interference studies demonstrated that prior infection of equine fibroblasts with EIAV did not alter the ability of vMA-1c to infect and kill these cells. In similar studies in a canine fibroblast cell line, receptor interference did occur. vMA-1c infection of equine fibroblasts was also associated with large quantities of unintegrated viral DNA, a well-established hallmark of retroviral superinfection. Cloning of the vMA-1c genome identified nucleotide changes that would result in at least one amino acid change in all viral proteins. A chimeric infectious molecular clone containing the vMA-1c tat, S2, and env open reading frames recapitulated most of the characteristics of vMA-1c, including superinfection, fibroblast killing, and fusogenic activity. In summary, in vitro selection for a strain of EIAV that rapidly killed cells resulted in the generation of a virus that was able to superinfect these cells, presumably by the use of a novel mechanism of cell entry. This phenotype mapped to the 3′ half of the genome.

Characterization of deoxyribozymes with site-specific oxidative cleavage activity against DNA obtained by in vitro selection

2016 ◽  
Vol 14 (7) ◽  
pp. 2347-2351 ◽  
Author(s):  
Ming-Qi Wang ◽  
Juan Dong ◽  
Huafan Zhang ◽  
Zhuo Tang
Keyword(s):  
In Vitro Selection ◽  
Oxidative Cleavage ◽  
Site Specific ◽  
New Class ◽  
Cleavage Activity ◽  
A Site

We have generated a new class of deoxyribozymes that required Mn2+ and Cu2+ to catalyze a site-specific oxidative cleavage of DNA.

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