Correction to: Evaluation of Potential DNA-Damaging Effects of Nitenpyram and Imidacloprid in Human U937-Cells Using a New Statistical Approach to Analyse Comet Data

Abstract Even if the two neonicotinoids nitenpyram and imidacloprid have been considered safe for humans, their potential genotoxicity still remains a matter of discussion. The DNA-damaging effects of these two compounds were therefore evaluated in a lymphoma cell line of human origin (U-937) using the comet assay after 3-h exposure to up to 50 μM, with or without metabolic activation using S9 from human liver. The comet data were analysed using a traditional one-way ANOVA after pooling the data on cellular level, and a new alternative approach we have called Uppsala Comet Data Analysis Strategy (UCDAS). UCDAS is a proportional odds model tailored to continuous outcomes, taking the number of pooled cultures, slides and cells into consideration in the same analysis. To the best of our knowledge, the UCDAS approach when analysing comet data has never been presented before. Without metabolic activation, no increase in DNA damage was observed in the neonicotinoide-exposed cells. Nitenpyram was also without DNA-damaging effects when S9 was added. However, in the presence of S9, imidacloprid was found to increase the level of DNA damage. Whereas the ANOVA showed an increase (P < 0.001) both at 5 and 50 μM, UCDAS showed an increase only at the lowest concentration (P < 0.001). Based on these findings, the two neonicotinoids seem to be of little concern when it comes to their potential genotoxicity. However, since the U-937 cells were rather resistant to our positive controls, they may not be the best cells to use when evaluating potential genotoxicity of chemicals.

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Enhancement of Plasminogen Binding to U937 Cells and Fibrin by Complestatin

Thrombosis and Haemostasis ◽

10.1055/s-0038-1655921 ◽

1997 ◽

Vol 77 (01) ◽

pp. 137-142 ◽

Cited By ~ 20

Author(s):

Kiyoshi Tachikawa ◽

Keiji Hasurni ◽

Akira Endo

Keyword(s):

Binding Site ◽

Binding Affinity ◽

Blood Cells ◽

Aminocaproic Acid ◽

U937 Cells ◽

Biochemical Basis ◽

Equilibrium Binding ◽

Pharmacological Stimulation ◽

Endogenous Fibrinolysis ◽

Stimulation Of

SummaryPlasminogen binds to endothelial and blood cells as well as to fibrin, where the zymogen is efficiently activated and protected from inhibition by α2-antiplasmin. In the present study we have found that complestatin, a peptide-like metabolite of a streptomyces, enhances binding of plasminogen to cells and fibrin. Complestatin, at concentrations ranging from 1 to 5 μM, doubled 125I-plasminogen binding to U937 cells both in the absence and presence of lipoprotein(a), a putative physiological competitor of plasminogen. The binding of 125I-plasminogen in the presence of complestatin was abolished by e-aminocaproic acid, suggesting that the lysine binding site(s) of the plasminogen molecule are involved in the binding. Equilibrium binding analyses indicated that complestatin increased the maximum binding of 125I-plasminogen to U937 cells without affecting the binding affinity. Complestatin was also effective in increasing 125I-plasminogen binding to fibrin, causing 2-fold elevation of the binding at ~1 μM. Along with the potentiation of plasminogen binding, complestatin enhanced plasmin formation, and thereby increased fibrinolysis. These results would provide a biochemical basis for a pharmacological stimulation of endogenous fibrinolysis through a promotion of plasminogen binding to cells and fibrin.

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Statistical Approach to Modeling Reduced Shear Capacity of Corrosion-Damaged Reinforced Concrete Beams

Practice Periodical on Structural Design and Construction ◽

10.1061/(asce)sc.1943-5576.0000564 ◽

2021 ◽

Vol 26 (2) ◽

pp. 04020073

Author(s):

Mahmoodreza Soltani ◽

Adham Abu-Abaileh ◽

Bryan Scott Rowe

Keyword(s):

Reinforced Concrete ◽

Statistical Approach ◽

Concrete Beams ◽

Shear Capacity ◽

Reinforced Concrete Beams

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Delivering Sustainable Water Systems Using Kalman Filter and Statistical Approach

Journal of Applied Information, Communication and Technology ◽

10.33555/ejaict.v4i1.82 ◽

2017 ◽

Vol 4 (1) ◽

pp. 41-52

Author(s):

Dedy Loebis

Keyword(s):

Kalman Filtering ◽

Statistical Approach ◽

Water Systems ◽

Data Sets ◽

Pressure Data ◽

Burst Event ◽

Sample Data ◽

Other Information ◽

Supply Systems ◽

Frequency Features

This paper presents the results of work undertaken to develop and test contrasting data analysis approaches for the detection of bursts/leaks and other anomalies within wate r supply systems at district meter area (DMA)level. This was conducted for Yorkshire Water (YW) sample data sets from the Harrogate and Dales (H&D), Yorkshire, United Kingdom water supply network as part of Project NEPTUNE EP/E003192/1 ). A data analysissystem based on Kalman filtering and statistical approach has been developed. The system has been applied to the analysis of flow and pressure data. The system was proved for one dataset case and have shown the ability to detect anomalies in flow and pres sure patterns, by correlating with other information. It will be shown that the Kalman/statistical approach is a promising approach at detecting subtle changes and higher frequency features, it has the potential to identify precursor features and smaller l eaks and hence could be useful for monitoring the development of leaks, prior to a large volume burst event.

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