Efficiency of some molecular markers linked to rhizomania resistance gene (Rz 1 ) for marker assisted selection in sugar beet

2015 ◽  
Vol 18 (5) ◽  
pp. 319-323 ◽  
Author(s):  
Peyman Norouzi ◽  
Mohammad Sabzehzari ◽  
Hasan Zeinali
Keyword(s):  
Molecular Markers ◽  
Sugar Beet ◽  
Resistance Gene ◽  
Marker Assisted Selection ◽  
Rhizomania Resistance

Confirmation of some SCAR molecular markers linked to Rhizomania resistance gene (Rz1) in sugar beet

2017 ◽  
Vol 77 (2) ◽  
pp. 312
Author(s):  
Peyman Norouzi ◽  
Seyed Bagher Mahmoudi ◽  
Saeed Darabi ◽  
Mozhdeh Kakueinezhad
Keyword(s):  
Molecular Markers ◽  
Sugar Beet ◽  
Resistance Gene ◽  
Rhizomania Resistance

Resistance gene analogues are clustered on chromosome 3 of sugar beet and cosegregate with QTL for rhizomania resistance

Genome ◽  
2007 ◽  
Vol 50 (1) ◽  
pp. 61-71 ◽  
Author(s):  
Jens Christoph Lein ◽  
Katrin Asbach ◽  
Yanyan Tian ◽  
Daniela Schulte ◽  
Chunyan Li ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Sugar Beet ◽  
Resistance Gene ◽  
Resistance Genes ◽  
Bac Library ◽  
Artificial Chromosome ◽  
Chromosome 3 ◽  
Resistance Locus ◽  
Resistant Varieties ◽  
Rhizomania Resistance

Worldwide, rhizomania is the most important disease of sugar beet. The only way to control this disease is to use resistant varieties. Four full-length resistance gene analogues (RGAs) from sugar beet (cZR-1, cZR-3, cZR-7, and cZR-9) were used in this study. Their predicted polypeptides carry typical nucleotide-binding sites (NBSs) and leucin-rich repeat (LRR) regions, and share high homology to various plant virus resistance genes. Their corresponding alleles were cloned and sequenced from a rhizomania resistant genotype. The 4 RGAs were mapped as molecular markers, using sequence-specific primers to determine their linkage to the rhizomania resistance locus Rz1 in a population segregating for rhizomania resistance. One cZR-3 allele, named Rz-C, together with 5 other molecular markers, mapped to the Rz1 locus on chromosome 3 and cosegregated with quantitative trait loci for rhizomania resistance. After screening a bacterial artificial chromosome (BAC) library, 25 cZR-3-positive BACs were identified. Of these, 15 mapped within an interval of approximately 14 cM on chromosome 3, in clusters close to the Rz1 locus. Rz-C differentiates between susceptible and resistant beet varieties, and its transcripts could be detected in all rhizomania resistant varieties investigated. The potential of this RGA marker for cloning of rhizomania resistance genes is discussed.

Development of Molecular Markers Closely Linked to the Potato Leafroll Virus Resistance Gene, Rlr etb , for use in Marker-Assisted Selection

2016 ◽  
Vol 93 (3) ◽  
pp. 203-212 ◽  
Author(s):  
Joseph C. Kuhl ◽  
Richard G. Novy ◽  
Jonathan L. Whitworth ◽  
Margaret S. Dibble ◽  
Brian Schneider ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Resistance Gene ◽  
Virus Resistance ◽  
Marker Assisted Selection ◽  
Potato Leafroll Virus ◽  
Leafroll Virus ◽  
Virus Resistance Gene

scholarly journals Different molecular markers to identify the fertility of sugar beet monogerm germplasm resources

2021 ◽  
Author(s):  
Haoqi Shi ◽  
Zhi Pi ◽  
Zedong Wu
Keyword(s):  
Molecular Markers ◽  
Nucleotide Sequence ◽  
Sugar Beet ◽  
Marker Assisted Selection ◽  
Enzyme Digestion ◽  
Germplasm Resources ◽  
Single Nucleotide

Abstract Experiments were conducted to validate different molecular markers associated with sugar beet breeding using available sugar beet monogerm germplasm resources to explore their effectiveness in different sugar beet populations. We used multiple primer pairs to amplify sugar beet monogerm sterile and maintainer lines in order to verify their polymorphism. For the nucleus Rf1 locus genotype enzyme digestion was also required to verify. The results showed that three pairs of primers, TR1, s17 and 11E8M4S, produced polymorphism when amplifying sugar beet sterile and maintainer lines; primers o7 and AB-18, although polymorphic, did not correlate significantly with sugar beet fertility; primer cpSSR-2 did not produce significant band differences when amplifying sterile and maintainer lines, however, the number of single nucleotide sequence repeats of base A needed to be further verified as a basis for differentiating sugar beet fertility. The polymorphism of 15 pairs of primers related to sugar beet fertility identification was verified and TR1, s17 and 11E8M4S could be used to differentiate sugar beet sterile lines from the maintainer lines and used for subsequent Marker-Assisted Selection (MAS).

scholarly journals Confirmation of Rpp Genes Conferring Resistance to Asian Soybean Rust and Mapping of Rpp1 Allele from PI 594723

2021 ◽  
Author(s):  
Daniela Meira ◽  
Vinícius de B. Bez Batti ◽  
Leomar G. Woyann ◽  
Anderson Simionato Milioli ◽  
Antonio Henrique Bozi ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Glycine Max ◽  
Resistance Gene ◽  
Marker Assisted Selection ◽  
Snp Markers ◽  
Soybean Rust ◽  
Selection Strategies ◽  
Asian Soybean Rust ◽  
Resistant Sources ◽  
Kasp Markers

Abstract In this study, we aim to develop and validate KASP molecular markers in soybean populations for Asian soybean rust (ASR) resistance gene Rpp1 (PI 200492, PI 594538A, PI 587880A), identify the gene hypothetically present in PI 594723, and validate KASP markers for Rpp2 (PI 230970), Rpp3 (PI 506764), Rpp4 (PI 459025A), and Rpp5 (PI 506764, PI 200487). Ten F2 soybean (Glycine max (L.) Merrill) populations derived from crosses between rust-susceptible (55I57RSF IPRO, 63I64RSF IPRO) x rust-resistant sources (PI 200492, PI 594738A, PI 587880A, PI 594723, PI 230970, PI 506764, PI 459025A and PI 200487) were evaluated. All F2 plants were individually evaluated in field conditions for ASR phenotypic reactions, classified according to sporulation level. SNP markers were developed according to markers associated with Rpp genes available at the SoyBase, using KASP methodology. Based on a slight difference in map position and different phenotypic disease reactions of PI 200492, the authors suggest that PI 594723 carries a resistance gene Rpp1-b. The Rpp1-b gene from PI 594723 was mapped in Chr 18 in a 12.4 cM region. The PIs carrying Rpp1-b (PI 594723, PI 587880A, and 594538A) showed strong resistance to ASR compared to the lines carrying Rpp1 (PI 200492). A total of 26 KASP markers were significantly associated (P < 0.01) with ASR. Among those, M1, M5 and M6 (Rpp1), M13 and M14 (Rpp2), M16, M17 and M20 (Rpp3), M25 and M26 (Rpp4), and M27 and M28 (Rpp5) have the potential to be used in marker-assisted selection strategies.

Molecular markers linked to the blast resistance gene Pi-z in rice for use in marker-assisted selection

2003 ◽  
Vol 107 (6) ◽  
pp. 1014-1020 ◽  
Author(s):  
C. A. Conaway-Bormans ◽  
M. A. Marchetti ◽  
C. W. Johnson ◽  
A. M. McClung ◽  
W. D. Park
Keyword(s):  
Molecular Markers ◽  
Resistance Gene ◽  
Marker Assisted Selection ◽  
Blast Resistance ◽  
Blast Resistance Gene

QTL mapping of BNYVV resistance from the WB41 source in sugar beet

Genome ◽  
2005 ◽  
Vol 48 (2) ◽  
pp. 279-285 ◽  
Author(s):  
Sara Gidner ◽  
Britt-Louise Lennefors ◽  
Nils-Otto Nilsson ◽  
Jan Bensefelt ◽  
Evert Johansson ◽  
...  
Keyword(s):  
Sugar Beet ◽  
Qtl Mapping ◽  
Resistance Gene ◽  
Beta Vulgaris ◽  
Incomplete Penetrance ◽  
Resistant Varieties ◽  
Major Resistance Gene ◽  
Rhizomania Resistance ◽  
Chromosome Iii ◽  
Greenhouse Tests

The most important rhizomania-resistance gene in sugar beet is the Rz1 gene from the Holly Sugar Company in California, the source widely used to breed partially resistant varieties. Other important gene sources are WB41 and WB42, which both originate from Beta vulgaris subsp. maritima collected in Denmark, and which have been reported to be similar. The major resistance gene in WB42 is known as Rz2. We studied the resistance in WB41 and used markers to map the major resistance gene in this source, which we call Rz3. It was identified on chromosome III. This is the chromosome that Rz1 and Rz2 have been mapped to. Data from greenhouse tests and ELISA showed that Rz3 had incomplete penetrance, with heterozygotes varying widely in resistance levels. The involvement of additional minor genes in the strong resistance of the original WB41 source cannot be excluded.Key words: BNYVV, WB41, rhizomania, QTL, Beta vulgaris, AFLP, SSR.

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