Confirmation of some SCAR molecular markers linked to Rhizomania resistance gene (Rz1) in sugar beet

Worldwide, rhizomania is the most important disease of sugar beet. The only way to control this disease is to use resistant varieties. Four full-length resistance gene analogues (RGAs) from sugar beet (cZR-1, cZR-3, cZR-7, and cZR-9) were used in this study. Their predicted polypeptides carry typical nucleotide-binding sites (NBSs) and leucin-rich repeat (LRR) regions, and share high homology to various plant virus resistance genes. Their corresponding alleles were cloned and sequenced from a rhizomania resistant genotype. The 4 RGAs were mapped as molecular markers, using sequence-specific primers to determine their linkage to the rhizomania resistance locus Rz1 in a population segregating for rhizomania resistance. One cZR-3 allele, named Rz-C, together with 5 other molecular markers, mapped to the Rz1 locus on chromosome 3 and cosegregated with quantitative trait loci for rhizomania resistance. After screening a bacterial artificial chromosome (BAC) library, 25 cZR-3-positive BACs were identified. Of these, 15 mapped within an interval of approximately 14 cM on chromosome 3, in clusters close to the Rz1 locus. Rz-C differentiates between susceptible and resistant beet varieties, and its transcripts could be detected in all rhizomania resistant varieties investigated. The potential of this RGA marker for cloning of rhizomania resistance genes is discussed.

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Efficiency of some molecular markers linked to rhizomania resistance gene (Rz 1 ) for marker assisted selection in sugar beet

Journal of Crop Science and Biotechnology ◽

10.1007/s12892-015-0033-9 ◽

2015 ◽

Vol 18 (5) ◽

pp. 319-323 ◽

Cited By ~ 4

Author(s):

Peyman Norouzi ◽

Mohammad Sabzehzari ◽

Hasan Zeinali

Keyword(s):

Molecular Markers ◽

Sugar Beet ◽

Resistance Gene ◽

Marker Assisted Selection ◽

Rhizomania Resistance

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Molecular markers linked to rhizomania resistance in sugar beet, Beta vulgaris, from two different sources map to the same linkage group

Plant Breeding ◽

10.1111/j.1439-0523.1997.tb01022.x ◽

1997 ◽

Vol 116 (5) ◽

pp. 401-408 ◽

Cited By ~ 11

Author(s):

G. Giorio ◽

M. Gallitelli ◽

F. Carriero

Keyword(s):

Molecular Markers ◽

Linkage Group ◽

Sugar Beet ◽

Beta Vulgaris ◽

Rhizomania Resistance ◽

Different Sources

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Identification and mapping of random amplified polymorphic DNA markers linked to a rhizomania resistance gene in sugar beet (Beta vulgaris L.) by bulked segregant analysis

Plant Breeding ◽

10.1111/j.1439-0523.1996.tb00936.x ◽

1996 ◽

Vol 115 (5) ◽

pp. 371-377 ◽

Cited By ~ 20

Author(s):

F. Pelsy ◽

D. Merdinoglu

Keyword(s):

Sugar Beet ◽

Resistance Gene ◽

Beta Vulgaris ◽

Dna Markers ◽

Bulked Segregant Analysis ◽

Random Amplified Polymorphic Dna ◽

Rhizomania Resistance

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QTL mapping of BNYVV resistance from the WB41 source in sugar beet

Genome ◽

10.1139/g04-108 ◽

2005 ◽

Vol 48 (2) ◽

pp. 279-285 ◽

Cited By ~ 62

Author(s):

Sara Gidner ◽

Britt-Louise Lennefors ◽

Nils-Otto Nilsson ◽

Jan Bensefelt ◽

Evert Johansson ◽

...

Keyword(s):

Sugar Beet ◽

Qtl Mapping ◽

Resistance Gene ◽

Beta Vulgaris ◽

Incomplete Penetrance ◽

Resistant Varieties ◽

Major Resistance Gene ◽

Rhizomania Resistance ◽

Chromosome Iii ◽

Greenhouse Tests

The most important rhizomania-resistance gene in sugar beet is the Rz1 gene from the Holly Sugar Company in California, the source widely used to breed partially resistant varieties. Other important gene sources are WB41 and WB42, which both originate from Beta vulgaris subsp. maritima collected in Denmark, and which have been reported to be similar. The major resistance gene in WB42 is known as Rz2. We studied the resistance in WB41 and used markers to map the major resistance gene in this source, which we call Rz3. It was identified on chromosome III. This is the chromosome that Rz1 and Rz2 have been mapped to. Data from greenhouse tests and ELISA showed that Rz3 had incomplete penetrance, with heterozygotes varying widely in resistance levels. The involvement of additional minor genes in the strong resistance of the original WB41 source cannot be excluded.Key words: BNYVV, WB41, rhizomania, QTL, Beta vulgaris, AFLP, SSR.

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