Callus Induction and Regeneration from Anther Cultures of Indonesian Indica Black Rice Cultivar

2019 ◽  
Vol 23 (1) ◽  
pp. 21-28
Author(s):  
Anisa Maharani ◽  
Wahyu Indra Duwi Fanata ◽  
Faida Nur Laeli ◽  
Kyung-Min Kim ◽  
Tri Handoyo
Keyword(s):  
Callus Induction ◽  
Rice Cultivar ◽  
Black Rice ◽  
Anther Cultures

scholarly journals Enhancement of Regeneration Efficiency through Callus Induction Media Using 2,4-Dicholorophenoxyacetic acid in Indica Rice (Oryza sativa L. var. Ciherang)

2017 ◽  
Vol 18 (2) ◽  
pp. 91
Author(s):  
Kunti Anis Azizah ◽  
Didik Pudji Restanto ◽  
Bambang Sugiharto
Keyword(s):  
Oryza Sativa ◽  
Callus Induction ◽  
Embryogenic Callus ◽  
Oryza Sativa L ◽  
Indica Rice ◽  
Rice Variety ◽  
Rice Cultivar ◽  
Medium Composition ◽  
Regeneration Frequency ◽  
Optimum Result

Indica rice variety Ciherang is the most planted variety in Indonesia, but the micropropagation technique is restricted because it is known has low regeneration frequency and included as recalcitrant cultivar for tissue culture and tranformation activities. One of solution to resolve that problem is developing a technique of somatic embryogenesis in callus of ciherang rice cultivar. The aims of study were to determine medium composition for inducting embryogenic callus in Ciherang rice and to know the effectivity of rice regeneration using callus as explant. The methods were included induction of embryogenic callus in callus induction media (CIM) containing MS basal, Proline 600 mg/l, Casein Hidrolisat 300 mg/l, phytagel 2,5 g/l, BAP 0.25 mg/l, sukrosa 30 %, and 2,4-D in different concentration, from 2,4-D 0 mg/l as control (CIM 1), 2,4-D 2 mg/l (CIM 2), 2,4-D 3 mg/l (CIM 3), and 2,4-D 4 mg/l (CIM 4). It then be continued to regenerate the calli in RM 1 medium containing MS basal, NAA 0,2 mg/l, Kinetin 2 mg/l, Agarose 10 g/l, and sukrosa 30 %, pH 5,8 for six days in dark and RM2 medium containing MS basal , NAA 0,2 mg/l, Kinetin 2 mg/l, Agarose 8 g/l, sukrosa 30 %, pH 5,8 in light room. Results showed CIM 4 medium using 2,4-D 4 mg/l gave optimum result in calli induction with procentage 57,63% and CIM 3 using 2,4-D 3 mg/l gave optimum result in embryonic calli induction with procentage 53,63%. Result of embryonic calli gave regeneration frequency procentage is 9,6%. The total planlet obtained after five weeks old in regeneration medum were 11 planlets ready for aclimatization. Keywords: Somatic embryo, Indica rice (Oryza sativa L. var. Ciherang), Callus

scholarly journals ANALISIS SESAR-SESAR MAJOR SEMENANJUNG MALAYSIA DENGAN TEKNIK GRAVITI DEKONVOLUSI EULER 3D

2021 ◽  
Vol 83 (4) ◽  
pp. 137-150
Author(s):  
Nurul Fairuz Diyana Bahrudin ◽  
Umar Hamzah ◽  
Wan Zuhairi Wan Yaacob
Keyword(s):  
Shoot Regeneration ◽  
Callus Induction ◽  
Upland Rice ◽  
Rice Cultivar ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Partial Desiccation ◽  
Upland Rice Cultivar ◽  
Improvement Programs ◽  
Rice Improvement

The application of biotechnology in upland rice improvement programs depends on the availability of efficient regeneration protocols.  Although protocols for shoot regeneration of upland rice are available, none has been reported for pigmented cultivars.  This study reports on a protocol for callus induction and regeneration of Tadong, a pigmented upland rice cultivar from Sabah.  For callus induction, immature embryos were cultured on media containing 2,4-Dichlorophenoxyacetic (2,4-D) at various concentrations (0 – 2.5 mg/L) and on different types of media (MS; MSB5; N6B5; N6).  To induce shoot regeneration, callus explants were cultured on MS medium supplemented with combinations of 6-Benzylaminopurine (BAP) at various concentrations (0 – 3.0 mg/L) and 1-Naphthaleneacetic acid (NAA) at 1.0 mg/L.  To induce shoot development, callus explants were pre-treated with Thidiazuron (TDZ) at various concentrations (0-1.0 mg/l) and exposed to different desiccation periods (0 – 72 hours).  2,4-Dichlorophenoxyacetic at 2.5 mg/L and N6B5 medium resulted in the highest percentages of explant forming callus which were 60.3 ± 17.0 % and 58.7 ± 9.8 % respectively.   The regeneration media failed to induce shoot on callus explants, instead, green spots were formed on the surface of the callus.  The green spots were stimulated to develop into shoots when the callus explants were pre-treated with 0.5 mg/L TDZ or exposed to partial desiccation for 24 h, the percentages of explant forming shoot were 35.7 ± 4.8 % and 47.7 ± 6.8 % respectively.   Shoots developed into complete plants on hormone-free MS medium and acclimatized. 

scholarly journals CALLUS FORMATION AND PLANT REGENERATION FROM IMMATURE EMBRYOS OF PIGMENTED UPLAND RICE (Oryza sativa cv. Tadong)

2021 ◽  
Vol 83 (4) ◽  
pp. 91-100
Author(s):  
Hoo Kah Yan ◽  
Lee Ping Chin ◽  
Mariam A. Latip ◽  
Noumie Surugau ◽  
Zaleha A. Aziz
Keyword(s):  
Shoot Regeneration ◽  
Callus Induction ◽  
Upland Rice ◽  
Callus Formation ◽  
Rice Cultivar ◽  
Immature Embryos ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Improvement Programs ◽  
Rice Improvement

  The application of biotechnology in upland rice improvement programs depends on the availability of efficient regeneration protocols.  Although protocols for shoot regeneration of upland rice are available, none has been reported for pigmented cultivars.  This study reports on a protocol for callus induction and regeneration of Tadong, a pigmented upland rice cultivar from Sabah.  For callus induction, immature embryos were cultured on media containing 2,4-Dichlorophenoxyacetic (2,4-D) at various concentrations (0 – 2.5 mg/L) and on different types of media (MS; MSB5; N6B5; N6).  To induce shoot regeneration, callus explants were cultured on MS medium supplemented with combinations of 6-Benzylaminopurine (BAP) at various concentrations (0 – 3.0 mg/L) and 1-Naphthaleneacetic acid (NAA) at 1.0 mg/L.  To induce shoot development, callus explants were pre-treated with Thidiazuron (TDZ) at various concentrations (0-1.0 mg/l) and exposed to different desiccation periods (0 – 72 hours).  2,4-Dichlorophenoxyacetic at 2.5 mg/L and N6B5 medium resulted in the highest percentages of explant forming callus which were 60.3 ± 17.0 % and 58.7 ± 9.8 % respectively.   The regeneration media failed to induce shoot on callus explants, instead, green spots were formed on the surface of the callus.  The green spots were stimulated to develop into shoots when the callus explants were pre-treated with 0.5 mg/L TDZ or exposed to partial desiccation for 24 h, the percentages of explant forming shoot were 35.7 ± 4.8 % and 47.7 ± 6.8 % respectively.   Shoots developed into complete plants on hormone-free MS medium and acclimatized.

scholarly journals The Effects of Temperature on Callus Induction and Regeneration in Selected Malaysian Rice Cultivar Indica

2018 ◽  
Vol 47 (11) ◽  
pp. 2647-2655 ◽  
Author(s):  
Suraiya binte Mostafiz ◽  
Alina Wagiran ◽  
Nur Syafiqoh Johan ◽  
Nur Saliha Abdullah Zulkifli ◽  
Ja Ming Ng
Keyword(s):  
Callus Induction ◽  
Rice Cultivar ◽  
Effects Of Temperature

scholarly journals Effects of Different Nitrogen Levels and Planting Densities on the Quality and Yield of the Black Rice Cultivar ‘Shinnongheugchal’

2016 ◽  
Vol 61 (2) ◽  
pp. 79-86
Author(s):  
In-Sok Lee ◽  
◽  
Deok-Ryeol Lee ◽  
Seung-Hyun Cho ◽  
Song-Yi Lee ◽  
...  
Keyword(s):  
Rice Cultivar ◽  
Black Rice ◽  
Nitrogen Levels

The γ-oryzanol Content of Thai Rice Cultivars and the Effects of Gamma Irradiation on the γ-oryzanol Content of Germinated Thai Market Rice

2020 ◽  
Vol 36 (05) ◽  
pp. 812-818
Author(s):  
Chakorn Chinvongamorn ◽  
Sompong Sansenya
Keyword(s):  
Gamma Irradiation ◽  
Rice Cultivar ◽  
Basic Knowledge ◽  
Rice Cultivars ◽  
Red Rice ◽  
Black Rice ◽  
Gamma Irradiated ◽  
Colored Rice

Thirty-nine Thai rice cultivars, including 17 non-colored rice cultivars and 22 colored rice cultivars (black rice cultivars and red rice cultivars), were investigated for γ-oryzanol content. The γ-oryzanol content of non-colored rice cultivars and colored rice cultivars was found to be 226.40 ± 17.50 μg/g to 411.80 ± 19.80 μg/g and 295.80 ± 15.40 μg/g to 459.80 ± 6.60 μg/g, respectively. Black rice cultivars seem to have higher γ-oryzanol content than do red rice and non-colored rice cultivars. The highest γ-oryzanol levels were identified in Khao Jao Dam Sa-Nit, a black rice cultivar, which contained 1.55 and 2.03 times more γ-oryzanol than Khao Man Bpoo, a red rice cultivar with lowest γ-oryzanol levels, and Khao Gor Kor 57, a non-colored rice cultivar with lowest γ-oryzanol levels, respectively. For further investigation, the effects of gamma irradiation on the γ-oryzanol content of four germinated Thai market rice cultivars, Khao Pathum Thani 1, Khao Gor Kor 31, Khao Gor Kor 41, and Khao Gor Kor 57, was investigated. The highest increase in γ-oryzanol content among the gamma irradiated rice cultivars was identified in Khao Pathum Thani 1 and Khao Gor Kor 31, which had γ-oryzanol levels approximately 1.27 times higher than that of the control rice (non-gamma irradiated rice). Our report contains basic knowledge about creating new rice lines that are rich in highly beneficial nutrients.

scholarly journals Relationships between cold resistance at the booting stage and callus induction of cultivars in rice anther cultures.

1986 ◽  
Vol 55 (4) ◽  
pp. 542-543
Author(s):  
Takahiro KATO ◽  
Tiemi NAKAMURA ◽  
Kazumi HATTORI ◽  
Eizo MAEDA
Keyword(s):  
Callus Induction ◽  
Cold Resistance ◽  
Booting Stage ◽  
Anther Cultures

scholarly journals Chemical and Sensory Properties of Black Rice Compared with Giza 177 Rice Cultivar

2019 ◽  
Vol 40 (OCTOBER- DECEMBER) ◽  
pp. 574-584
Author(s):  
Ayman M. Abouel-Yazeed ◽  
Mohamed A.A. Nassar ◽  
Ragab A. Ebaid ◽  
Mona A.M. Hassan
Keyword(s):  
Rice Cultivar ◽  
Sensory Properties ◽  
Black Rice
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