ANALISIS SESAR-SESAR MAJOR SEMENANJUNG MALAYSIA DENGAN  TEKNIK GRAVITI DEKONVOLUSI EULER 3D

The application of biotechnology in upland rice improvement programs depends on the availability of efficient regeneration protocols. Although protocols for shoot regeneration of upland rice are available, none has been reported for pigmented cultivars. This study reports on a protocol for callus induction and regeneration of Tadong, a pigmented upland rice cultivar from Sabah. For callus induction, immature embryos were cultured on media containing 2,4-Dichlorophenoxyacetic (2,4-D) at various concentrations (0 – 2.5 mg/L) and on different types of media (MS; MSB5; N6B5; N6). To induce shoot regeneration, callus explants were cultured on MS medium supplemented with combinations of 6-Benzylaminopurine (BAP) at various concentrations (0 – 3.0 mg/L) and 1-Naphthaleneacetic acid (NAA) at 1.0 mg/L. To induce shoot development, callus explants were pre-treated with Thidiazuron (TDZ) at various concentrations (0-1.0 mg/l) and exposed to different desiccation periods (0 – 72 hours). 2,4-Dichlorophenoxyacetic at 2.5 mg/L and N6B5 medium resulted in the highest percentages of explant forming callus which were 60.3 ± 17.0 % and 58.7 ± 9.8 % respectively. The regeneration media failed to induce shoot on callus explants, instead, green spots were formed on the surface of the callus. The green spots were stimulated to develop into shoots when the callus explants were pre-treated with 0.5 mg/L TDZ or exposed to partial desiccation for 24 h, the percentages of explant forming shoot were 35.7 ± 4.8 % and 47.7 ± 6.8 % respectively. Shoots developed into complete plants on hormone-free MS medium and acclimatized.

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Indirect in vitro Regeneration of the Medicinal Plant, Aspilia africana, and Histological Assessment at Different Developmental Stages

Frontiers in Plant Science ◽

10.3389/fpls.2021.797721 ◽

2021 ◽

Vol 12 ◽

Author(s):

Denis Okello ◽

Sungyu Yang ◽

Richard Komakech ◽

Yuseong Chung ◽

Endang Rahmat ◽

...

Keyword(s):

Medicinal Plant ◽

Shoot Regeneration ◽

Callus Induction ◽

Developmental Stages ◽

Naphthaleneacetic Acid ◽

Ms Medium ◽

Regeneration Capacity ◽

Root Explants ◽

Indirect Regeneration

The medicinal plant, Aspilia africana, has been traditionally used in several African countries to treat many diseases such as tuberculosis, cough, inflammation, malaria, osteoporosis, and diabetes. In this study, we developed a protocol for in vitro propagation of A. africana using indirect shoot organogenesis from leaf and root explants of in vitro-grown seedlings and assessed the tissues at different developmental stages. The highest callus induction (91.9 ± 2.96%) from leaf explants was in the Murashige and Skoog (MS) medium augmented with 1.0 mg/L 6-Benzylaminopurine (BAP) and 1.0 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) while from root explants, the highest callus induction (92.6 ± 2.80%) was in the same plant tissue culture medium augmented with 0.5 mg/L BAP and 1.0 mg/L 2,4-D. The best shoot regeneration capacity from leaf-derived calli (i.e., 80.0 ± 6.23% regeneration percentage and 12.0 ± 6.23 shoots per callus) was obtained in medium augmented with 1.0 mg/L BAP and 0.05 mg/L α-Naphthaleneacetic acid (NAA); the best regeneration capacity for root-derived calli (i.e., 86.7 ± 6.24% shoot regeneration percentage and 14.7 ± 1.11 shoots per callus) was obtained in the MS medium augmented with 1.0 mg/L BAP, 0.05 mg/L NAA, and 0.1 mg/L Thidiazuron (TDZ). Regenerated plantlets developed a robust root system in 1/2 MS medium augmented with 0.1 mg/L NAA and had a survival rate of 93.6% at acclimatization. The in vitro regenerated stem tissue was fully differentiated, while the young leaf tissue consisted of largely unorganized and poorly differentiated cells with large intercellular airspaces typical of in vitro leaf tissues. Our study established a protocol for the indirect regeneration of A. africana and offers a basis for its domestication, large-scale multiplication, and germplasm preservation. To the best of our knowledge, this is the first study to develop an indirect regeneration protocol for A. africana and conduct anatomical assessment through the different stages of development from callus to a fully developed plantlet.

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In vitro regeneration performance of Corchorus olitorius

Journal of the Bangladesh Agricultural University ◽

10.3329/jbau.v8i1.6390 ◽

1970 ◽

Vol 8 (1) ◽

pp. 1-6 ◽

Cited By ~ 1

Author(s):

M Hoque ◽

KM Nasiruddin ◽

GKMN Haque ◽

GC Biswas

Keyword(s):

Shoot Regeneration ◽

Callus Induction ◽

Root Formation ◽

Plantlet Regeneration ◽

Ms Medium ◽

Corchorus Olitorius ◽

The Media ◽

Regenerated Plantlets

The experiment was conducted during May to December 2008 in the Biotechnology Laboratory of Bangladesh Agricultural University, Mymensingh to observe the callus induction, regeneration potentiality and to establish a suitable in vitro plantlet regeneration protocol of Corchorus olitorius. MS medium supplemented with different phytohormone concentrations and combinations were used to observe the callus induction, shoot regeneration and root formation ability of the cotyledon with attached petiole derived explant of three genotypes viz. O-9897, O-72 and OM-1. The highest callus induction (92.85%) was observed in O-9897 followed by O-72 (82.14%) in the MS media supplemented with 2.5 mg/L BAP + 0.5 mg/L IAA. Genotype O-9897 in MS media supplemented with 2.5 mg/L BAP + 0.5 mg/L IAA produced the highest percentage of shoot regenerants (83.33%) followed by O-72 (75.00%) in the media supplemented with 2.5 mg/L BAP + 0.5 mg/L IAA. The root formation from regenerants was the best on halfstrength of MS media supplemented with 0.6 mg/L IBA in genotype O-9897 (45.00%). The in vitro regenerated plantlets from the genotypes O-9897 could be established in the field. Therefore, the genotypes O-9897 of C. olitorius in MS media supplemented with 2.5 mg/L BAP + 0.5 mg/L IAA could be used for callus induction and shoot regeneration. Keywords: Regeneration; Phytohormone; Corchorus olitorius DOI: 10.3329/jbau.v8i1.6390J. Bangladesh Agril. Univ. 8(1): 1-6, 2010

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In vitro study of Tinospora cordifolia (Willd.) Miers (Menispermaceae)

Botanica Orientalis Journal of Plant Science ◽

10.3126/botor.v6i0.2918 ◽

2010 ◽

Vol 6 ◽

pp. 103-105 ◽

Cited By ~ 5

Author(s):

Aditi Singh ◽

Saroj K Sah ◽

Aunji Pradhan ◽

Sabari Rajbahak ◽

Niran Maharajan

Keyword(s):

Medicinal Plant ◽

Callus Induction ◽

Shoot Growth ◽

Tinospora Cordifolia ◽

Nodal Segments ◽

Naphthaleneacetic Acid ◽

Nodal Segment ◽

Root Induction ◽

Ms Medium

In vitro study was carried out in an important medicinal plant Tinospora cordifolia (Willd.) Miers belonging to the family: Menispermaceae. Vegetative parts such as stem, leaf and nodal explants were excised from an elite in vivo grown mature plant and thereafter cultured on Murashige-Skoog (MS) medium supplemented with different hormonal concentrations for callus induction and organogenesis. Callus formation occurred from nodal segments, leaf and inter-node explants when planted on different combinations of hormones. Tinospora cordifolia showed response for in vitro shoot growth from the nodal segment. The best shoot growth was observed on MS medium supplemented with kinetin (1.5 mg/l). Similarly, the best result for root induction was obtained on MS medium supplemented with 6-benzylaminopurine (1.0 mg/l) and naphthaleneacetic acid (2.5 mg/l). Key-words: callus induction; explants; medicinal plant; MS medium; tissue culture.DOI: 10.3126/botor.v6i0.2918 Botanica Orientalis - Journal of Plant Science (2009) 6: 103-105

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Establishment of a Rapid and Efficient Micropropagation System for Succulent Plant Haworthia turgida Haw.

HortScience ◽

10.21273/hortsci12056-17 ◽

2017 ◽

Vol 52 (9) ◽

pp. 1278-1282 ◽

Cited By ~ 4

Author(s):

Boling Liu ◽

Hongzhou Fang ◽

Chaorong Meng ◽

Ming Chen ◽

Qingdong Chai ◽

...

Keyword(s):

Callus Induction ◽

Adventitious Shoots ◽

Germplasm Conservation ◽

Leaf Explants ◽

Adventitious Shoot ◽

Induction Medium ◽

Naphthaleneacetic Acid ◽

Root Induction ◽

Ms Medium ◽

Succulent Plant

In the present study, the effect of plant growth regulators (PGRs) on callus regeneration, adventitious shoot differentiation, and root formation of Haworthia turgida Haw. was investigated. The greatest callus induction percentage (95.6%) was achieved with leaf explants inoculated on Murashige and Skoog (MS) medium with 1.0 mg·L−1 6-benzyladenine (BA) and 0.1 mg·L−1 1-naphthaleneacetic acid (NAA), and this callus induction medium supplemented with 2.5 mg·L−1 thidiazuron (TDZ) was optimal for callus proliferation. The maximum number of shoots (25.7) was obtained when the callus was cultured on MS medium supplemented with 1.0 mg·L−1 BA and 0.2 mg·L−1 2,4-dichlorophenoxyacetic acid (2,4-D). The highest number of roots per shoot (6.2) and highest rooting frequency (82.0%) were obtained when adventitious shoots were inoculated on MS medium with 0.05 mg·L−1 NAA. Regenerated plantlets were transferred to a mixture of vermiculite and soil and acclimated in a greenhouse. The survival rate of the transplanted plantlets was about 91.6%. The rate of ex vitro rooting was 83.3%, indicating that this technique is effective for root induction in H. turgida. This study has established a rapid and efficient micropropagation system that can be beneficial for commercial cultivation and germplasm conservation of H. turgida.

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Identification and characterization of drought responsive miRNAs in a drought tolerant upland rice cultivar KMJ 1-12-3

Plant Physiology and Biochemistry ◽

10.1016/j.plaphy.2019.01.029 ◽

2019 ◽

Vol 137 ◽

pp. 62-74 ◽

Cited By ~ 7

Author(s):

Jay Prakash Awasthi ◽

Tilak Chandra ◽

Sagarika Mishra ◽

Shaifaly Parmar ◽

Birendra Prasad Shaw ◽

...

Keyword(s):

Upland Rice ◽

Rice Cultivar ◽

Drought Tolerant ◽

Upland Rice Cultivar ◽

Identification And Characterization

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QTLs associated with yield attributing traits under drought stress in upland rice cultivar of Assam

ORYZA- An International Journal on Rice ◽

10.5958/2249-5266.2017.00034.0 ◽

2017 ◽

Vol 54 (3) ◽

pp. 253 ◽

Cited By ~ 1

Author(s):

Vinay Sharma ◽

Rahul K Verma ◽

PC Dey ◽

SK Chetia ◽

AR Baruah ◽

...

Keyword(s):

Drought Stress ◽

Upland Rice ◽

Rice Cultivar ◽

Upland Rice Cultivar

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Determination of Upland Rice Cultivar Coefficient Specific Parameters for DSSAT (Version 4.7)-CERES-Rice Crop Simulation Model and Evaluation of the Crop Model under Different Temperature Treatments conditions

American Journal of Plant Sciences ◽

10.4236/ajps.2021.125054 ◽

2021 ◽

Vol 12 (05) ◽

pp. 782-795

Author(s):

Shams Shaila Islam ◽

Ahmed Khairul Hasan

Keyword(s):

Simulation Model ◽

Upland Rice ◽

Rice Cultivar ◽

Crop Model ◽

Rice Crop ◽

Crop Simulation Model ◽

Crop Simulation ◽

Upland Rice Cultivar

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Callus Induction and Plantlet Regeneration in Blackgram (Vigna mungo L. Hepper)

Progressive Agriculture ◽

10.3329/pa.v19i2.16917 ◽

2013 ◽

Vol 19 (2) ◽

pp. 27-35 ◽

Cited By ~ 1

Author(s):

SA Mony ◽

MS Haque ◽

MA Karim ◽

SK Roy

Keyword(s):

Shoot Regeneration ◽

Callus Induction ◽

Callus Formation ◽

Plantlet Regeneration ◽

Shoot Tip ◽

Root Tip ◽

Ms Medium ◽

Plantlet Formation ◽

Rooting Medium ◽

Shoot Tip Explants

The present study was undertaken for callus induction and subsequent plantlet regeneration in blackgram. The study comprised of experiments for callus initiation, shoot regeneration and plantlet formation from cotyledon, hypocotyl, root tip and shoot tip explants. The effects of explants and different concentrations and combinations of BAP (0.0, 1.0, 2.5, 5.0 and 10 mg L-1) and NAA (0.0, 0.5, 1.0, 1.5 and 2.0 mg L-1)) on callus induction were investigated first. Among the explants, hypocotyls showed the best performance in callus formation (92.33%) when cultured on MS medium supplemented with 2.5 mg L-1) BAP and 1.5 mg L-1) NAA followed by cotyledon, shoot tip and root tip explants, respectively. The height percentage of shoot regeneration from the calli derived from hypocotyls (56.33%) was achieved in MS medium supplemented with 3.0 mg L-1) BAP and 0.3 mg L-1) NAA and 0.5 mg L-1) GA3. Calli from other explants had no shoot regeneration. The regenerated shoots were transferred to rooting medium supplemented with different concentrations of IBA and NAA. The high frequency (100 %) of rooting was observed with MS medium supplemented with 0.5 mg L-1) IBA. The rooted plants were transferred to pots for hardening.DOI: http://dx.doi.org/10.3329/pa.v19i2.16917 Progress. Agric. 19(2): 27 - 35, 2008

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Interaction between thidiazuron and Azospirillum brasilense on yield characteristics and productivity of rice

Revista Brasileira de Engenharia Agrícola e Ambiental ◽

10.1590/1807-1929/agriambi.v23n4p244-249 ◽

2019 ◽

Vol 23 (4) ◽

pp. 244-249 ◽

Cited By ~ 1

Author(s):

Fernando de S. Buzo ◽

Lucas M. Garé ◽

Orivaldo Arf ◽

José R. Portugal ◽

Flavia C. Meirelles ◽

...

Keyword(s):

Experimental Design ◽

Azospirillum Brasilense ◽

Foliar Application ◽

Upland Rice ◽

Block Design ◽

Rice Cultivar ◽

Crop Productivity ◽

Randomized Complete Block Design ◽

Individual Effects ◽

Upland Rice Cultivar

ABSTRACT In rice, the use of the bacterium Azospirillum brasilense has shown to be very useful for the crop, being able to increase the final productivity of the same or reduce the need for nitrogen in topdressing. It has recently been noted that there are other regulators that perform other functions, such as thidiazuron (TDZ), a cytokinin-like substance that has been shown to significantly increase upland rice productivity. Thus, the present work aimed to verify the effect of the interaction between the foliar application of A. brasilense and thidiazuron doses applied at the occasion of the tillering of the plants on the productive aspects and crop productivity. The experiment was carried out in the agricultural years of 2015/16 and 2016/17, at the UNESP Teaching, Research and Extension Farm belonging to the Engineering Faculty of Ilha Solteira - UNESP, located in the municipality of Selvíria, MS. The experimental design was a randomized complete block design with a 2 x 4 factorial scheme with four replicates. The treatments were the combination of the presence or absence of A. brasilense foliar application with doses of TDZ (0, 0.5, 1.0 and 1.5 g ha-1) applied to upland rice cultivar BRS Esmeralda. Based on the results obtained for plant height, number of panicles per square meter, total number of grains and fertility of spikelets, mass of 100 grains and grain yield, it was concluded that, in the two agricultural years observed, there were no interaction and individual effects of the foliar application of the bacteria, and of the TDZ doses used at the time of tillering in the BRS Esmeralda cultivar.

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