ANALISIS SESAR-SESAR MAJOR SEMENANJUNG MALAYSIA DENGAN TEKNIK GRAVITI DEKONVOLUSI EULER 3D

The application of biotechnology in upland rice improvement programs depends on the availability of efficient regeneration protocols. Although protocols for shoot regeneration of upland rice are available, none has been reported for pigmented cultivars. This study reports on a protocol for callus induction and regeneration of Tadong, a pigmented upland rice cultivar from Sabah. For callus induction, immature embryos were cultured on media containing 2,4-Dichlorophenoxyacetic (2,4-D) at various concentrations (0 – 2.5 mg/L) and on different types of media (MS; MSB5; N6B5; N6). To induce shoot regeneration, callus explants were cultured on MS medium supplemented with combinations of 6-Benzylaminopurine (BAP) at various concentrations (0 – 3.0 mg/L) and 1-Naphthaleneacetic acid (NAA) at 1.0 mg/L. To induce shoot development, callus explants were pre-treated with Thidiazuron (TDZ) at various concentrations (0-1.0 mg/l) and exposed to different desiccation periods (0 – 72 hours). 2,4-Dichlorophenoxyacetic at 2.5 mg/L and N6B5 medium resulted in the highest percentages of explant forming callus which were 60.3 ± 17.0 % and 58.7 ± 9.8 % respectively. The regeneration media failed to induce shoot on callus explants, instead, green spots were formed on the surface of the callus. The green spots were stimulated to develop into shoots when the callus explants were pre-treated with 0.5 mg/L TDZ or exposed to partial desiccation for 24 h, the percentages of explant forming shoot were 35.7 ± 4.8 % and 47.7 ± 6.8 % respectively. Shoots developed into complete plants on hormone-free MS medium and acclimatized.

Late Miocene megalake regressions in Eurasia

The largest megalake in the geological record formed in Eurasia during the late Miocene, when the epicontinental Paratethys Sea became tectonically-trapped and disconnected from the global ocean. The megalake was characterized by several episodes of hydrological instability and partial desiccation, but the chronology, magnitude and impacts of these paleoenvironmental crises are poorly known. Our integrated stratigraphic study shows that the main desiccation episodes occurred between 9.75 and 7.65 million years ago. We identify four major regressions that correlate with aridification events, vegetation changes and faunal turnovers in large parts of Europe. Our paleogeographic reconstructions reveal that the Paratethys was profoundly transformed during regression episodes, losing ~ 1/3 of the water volume and ~ 70% of its surface during the most extreme events. The remaining water was stored in a central salt-lake and peripheral desalinated basins while vast regions (up to 1.75 million km2) became emergent land, suitable for development of forest-steppe landscapes. The partial megalake desiccations match with climate, food-web and landscape changes throughout Eurasia, although the exact triggers and mechanisms remain to be resolved.

Magnetostratigraphic dating of late Miocene megalake regressions in Central Eurasia

<p>The largest megalake in the record formed in Eurasia during the late Miocene, when the epicontinental Paratethys Sea became tectonically-trapped and disconnected from the global ocean. The Paratethys megalake was characterized by several episodes of hydrological instability and partial desiccation, but the chronology, magnitude and impacts of these paleoenvironmental crises are poorly known. The Panagia section on the Taman Peninsula of Russia is the only place known to host a continuous sedimentary record of the late Miocene hydrological crises of Paratethys. Paleomagnetic measurements allow the development of a polarity pattern that can be used to date the regression events. The Panagia polarity pattern consists of 17 polarity intervals, 9 of normal polarity and 8 of reversed polarity, plus 4 additional short-term polarity fluctuations, that are inferred to correspond to the 11-7.5 Ma interval. We identified four major regressions that correlate with aridification events, vegetation changes and faunal turnovers in large parts of Europe. Our paleogeographic reconstructions reveal that Paratethys was profoundly transformed during the regression episodes, losing ~1/3 of the water volume and ~70% of its surface during the most extreme events. The remaining water was stored in a central salt-lake and peripheral desalinated basins while vast regions (up to 1.75 million km2) became emerged land, suitable for the development of forest-steppe landscapes. The dry episodes of the megalake match with climate, food-web and landscape changes throughout Eurasia but the exact triggers and mechanisms remain to be resolved.</p>

Combination of Plant Growth Regulators, Maltose, and Partial Desiccation Treatment Enhance Somatic Embryogenesis in Selected Malaysian Rice Cultivar

The development of efficient tissue culture protocol for somatic embryo would facilitate the genetic modification breeding program. The callus induction and regeneration were studied by using different parameters i.e., auxins, cytokinins, and desiccation treatment. Scanning electron microscopy and histological analysis were performed to identify the embryogenic callus for regeneration. The callus percentage results showed that MS (Murashige and Skoog) basal medium supplemented with 3 mg/L 2, 4-D and 30g/L maltose were the optimal callus induction medium for MR220 (80%) and MR220-CL2 (95%). The morphology of the embryogenic callus was confirmed by the SEM (Scanning Electron Microscopy) (presence of extracellular matrix surface network) and later by histological analysis. Finally, MS media supplemented with 0.5 mg/L NAA (Naphthalene Acetic Acid), 2 mg/L kin, and 1 mg/L BAP were selected as the optimum regeneration media treatment while callus desiccated for 48 h was proved to produce more plantlets in MR220 (60%) and MR220-CL2 (73.33%) compared to control treatment (without desiccation). The protocol presented here showed the necessity for the inclusion of partial desiccation as an important step in the tissue culture protocol of Malaysian indica rice genotypes in order to enhance their regeneration potential.