Cold-Active β-Galactosidases: Sources, Biochemical Properties and Their Biotechnological Potential

2016 ◽  
pp. 445-469 ◽  
Author(s):  
Hubert Cieśliński ◽  
Marta Wanarska ◽  
Anna Pawlak-Szukalska ◽  
Ewelina Krajewska ◽  
Monika Wicka ◽  
...  
Keyword(s):  
Biochemical Properties ◽  
Biotechnological Potential ◽  
Cold Active

A New Alkaliphilic Cold-Active Esterase from the Psychrophilic Marine Bacterium Rhodococcus sp.: Functional and Structural Studies and Biotechnological Potential

2014 ◽  
Vol 172 (6) ◽  
pp. 3054-3068 ◽  
Author(s):  
Concetta De Santi ◽  
Pietro Tedesco ◽  
Luca Ambrosino ◽  
Bjørn Altermark ◽  
Nils-Peder Willassen ◽  
...  
Keyword(s):  
Marine Bacterium ◽  
Structural Studies ◽  
Biotechnological Potential ◽  
Cold Active ◽  
Rhodococcus Sp

Biotechnological potential of psychrophilic microorganisms as the source of cold-active enzymes in food processing applications

3 Biotech ◽  
2021 ◽  
Vol 11 (11) ◽  
Author(s):  
Megha Kumari ◽  
Srichandan Padhi ◽  
Swati Sharma ◽  
Loreni Chiring Phukon ◽  
Sudhir P. Singh ◽  
...  
Keyword(s):  
Food Processing ◽  
Biotechnological Potential ◽  
Cold Active Enzymes ◽  
Cold Active

scholarly journals Draft Genome Sequence of Permafrost Bacterium Nesterenkonia sp. Strain PF2B19, Revealing a Cold Adaptation Strategy and Diverse Biotechnological Potential

2017 ◽  
Vol 5 (15) ◽  
Author(s):  
Purnima Singh ◽  
Neelam Kapse ◽  
Utpal Roy ◽  
Shiv Mohan Singh ◽  
P. K. Dhakephalkar
Keyword(s):  
Genome Sequence ◽  
Fatty Acid Biosynthesis ◽  
Draft Genome ◽  
Carotenoid Biosynthesis ◽  
Adaptation Strategy ◽  
Draft Genome Sequence ◽  
Psychrophilic Bacterium ◽  
Biotechnological Potential ◽  
Cold Active ◽  
Immense Potential

ABSTRACT Nesterenkonia sp. strain PF2B19, a psychrophilic bacterium, was isolated from 44,800-year-old permafrost. The draft genome sequence of this strain revealed the presence of genes involved in the production of cold active enzymes, carotenoid biosynthesis, fatty acid biosynthesis, and resistance to heavy metals. These results show the immense potential of the strain.

scholarly journals Cold Adaptation Strategies and the Potential of Psychrophilic Enzymes from the Antarctic Yeast, Glaciozyma antarctica PI12

2021 ◽  
Vol 7 (7) ◽  
pp. 528
Author(s):  
Nur Athirah Yusof ◽  
Noor Haza Fazlin Hashim ◽  
Izwan Bharudin
Keyword(s):  
Cold Adaptation ◽  
Multiple Stressors ◽  
Genome Structure ◽  
Adaptation Strategies ◽  
Biotechnological Potential ◽  
Psychrophilic Yeast ◽  
Cold Active Enzymes ◽  
Cold Active ◽  
Glaciozyma Antarctica Pi12 ◽  
And Function

Psychrophilic organisms possess several adaptive strategies which allow them to sustain life at low temperatures between −20 to 20 °C. Studies on Antarctic psychrophiles are interesting due to the multiple stressors that exist on the permanently cold continent. These organisms produce, among other peculiarities, cold-active enzymes which not only have tremendous biotechnological potential but are valuable models for fundamental research into protein structure and function. Recent innovations in omics technologies such as genomics, transcriptomics, proteomics and metabolomics have contributed a remarkable perspective of the molecular basis underpinning the mechanisms of cold adaptation. This review critically discusses similar and different strategies of cold adaptation in the obligate psychrophilic yeast, Glaciozyma antarctica PI12 at the molecular (genome structure, proteins and enzymes, gene expression) and physiological (antifreeze proteins, membrane fluidity, stress-related proteins) levels. Our extensive studies on G. antarctica have revealed significant insights towards the innate capacity of- and the adaptation strategies employed by this psychrophilic yeast for life in the persistent cold. Furthermore, several cold-active enzymes and proteins with biotechnological potential are also discussed.

scholarly journals Snake Venom L-Amino Acid Oxidases: Trends in Pharmacology and Biochemistry

2014 ◽  
Vol 2014 ◽  
pp. 1-19 ◽  
Author(s):  
Luiz Fernando M. Izidoro ◽  
Juliana C. Sobrinho ◽  
Mirian M. Mendes ◽  
Tássia R. Costa ◽  
Amy N. Grabner ◽  
...  
Keyword(s):  
Amino Acid ◽  
Platelet Aggregation ◽  
Enzymatic Activity ◽  
Snake Venom ◽  
Biochemical Properties ◽  
Mechanisms Of Action ◽  
Pharmacological Effects ◽  
Biotechnological Potential ◽  
Structure Activity ◽  
Induction Of Apoptosis

L-amino acid oxidases are enzymes found in several organisms, including venoms of snakes, where they contribute to the toxicity of ophidian envenomation. Their toxicity is primarily due to enzymatic activity, but other mechanisms have been proposed recently which require further investigation. L-amino acid oxidases exert biological and pharmacological effects, including actions on platelet aggregation and the induction of apoptosis, hemorrhage, and cytotoxicity. These proteins present a high biotechnological potential for the development of antimicrobial, antitumor, and antiprotozoan agents. This review provides an overview of the biochemical properties and pharmacological effects of snake venom L-amino acid oxidases, their structure/activity relationship, and supposed mechanisms of action described so far.

scholarly journals Biochemical Properties of a New Cold-Active Mono- and Diacylglycerol Lipase from Marine Member Janibacter sp. Strain HTCC2649

2014 ◽  
Vol 15 (6) ◽  
pp. 10554-10566 ◽  
Author(s):  
Dongjuan Yuan ◽  
Dongming Lan ◽  
Ruipu Xin ◽  
Bo Yang ◽  
Yonghua Wang
Keyword(s):  
Biochemical Properties ◽  
Diacylglycerol Lipase ◽  
Cold Active

Binding of 131I-Labeled Tissue-Type Plasminogen Activator on De-Endothelialized Lesions in Rabbits

1987 ◽  
Vol 26 (05) ◽  
pp. 224-228 ◽  
Author(s):  
Y. Isaka ◽  
H. Etani ◽  
K. Kimura ◽  
S. Yoneda ◽  
T. Kamada ◽  
...  
Keyword(s):  
Plasminogen Activator ◽  
Abdominal Aorta ◽  
Half Life ◽  
Balloon Catheter ◽  
Biochemical Properties ◽  
Tissue Type ◽  
Fibrin Deposition ◽  
Tissue Type Plasminogen Activator ◽  
High Affinity ◽  
Type Plasminogen Activator

Tissue-type plasminogen activator (t-PA) which has a high affinity for fibrin in the clot, was labeled with 131I by the iodogen method, and its binding to de-endothelialized lesions in the rabbit was measured to assess the detectability of thrombi. The de-endothelialized lesion was induced in the abdominal aorta with a Fogarty 4F balloon catheter. Two hours after the de-endothelialization, 131I-labeled t-PA (125 ± 46 μCi) was injected intravenously. The initial half-life of the agent in blood (n = 12) was 2.9 ± 0.4 min. The degree of binding of 131I-labeled t-PA to the de-endothelialized lesion was evaluated at 15 min (n = 6) or at 30 min (n = 6) after injection of the agent. In spite of the retention of the biochemical properties of 131I-labeled t-PA and the presence of fibrin deposition at the de-endothelialized lesion, the binding of t-PA to the lesion was not sufficiently strong. Lesion-to-control ratios (cpm/g/cpm/g) were 1.65 ± 0.40 (at 15 min) and 1.39 ± 1.31 (at 30 min), and lesion-to-blood ratios were 1.39 ± 0.32 (at 15 min) and 1.36 ± 0.23 (at 30 min). These results suggest that radiolabeled t-PA may be inappropriate as a radiopharmaceutical for the scintigraphic detection of a pre-existing thrombotic lesion.

Application of commercial test-systems to identify gram-negative facultatively anaerobic bacteria

2016 ◽  
Vol 3 (1) ◽  
pp. 43-48 ◽  
Author(s):  
V. Patyka ◽  
L. Butsenko ◽  
L. Pasichnyk
Keyword(s):  
Erwinia Amylovora ◽  
Anaerobic Bacteria ◽  
Biochemical Properties ◽  
Test System ◽  
Phytopathogenic Bacteria ◽  
Gram Negative ◽  
Test Systems ◽  
Facultatively Anaerobic ◽  
Microbiological Methods

Aim. To validate the suitability of commercial API 20E test-system (bioMerieux) for the identifi cation and characterization of facultative gram-negative phytopathogenic bacterial isolates. Methods. Conventional mi- crobiological methods, API 20E test-system (bioMerieux) according to the manufacturer’s instructions. Re- sults. The identifi cation results for Erwinia amylovora, Pectobacterium carotovorum and Pantoea agglome- rans isolates were derived from the conventional and API 20E test systems, which, were in line with the literature data for these species. The API 20E test-system showed high suitability for P. agglomerans isolates identifi cation. Although not all the species of facultatively anaerobic phytopathogenic bacteria may be identi- fi ed using API 20E test-system, its application will surely allow obtaining reliable data about their physiologi- cal and biochemical properties, valuable for identifi cation of bacteria, in the course of 24 h. Conclusions. The results of tests, obtained for investigated species while using API 20E test-system, and those of conventional microbiological methods coincided. The application of API 20E test-system (bioMerieux) ensures fast obtain- ing of important data, which may be used to identify phytopathogenic bacteria of Erwinia, Pectobacterium, Pantoea genera.

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