Optimization of protease and chitinase production by Bacillus cereus SV1 on shrimp shell waste using statistical experimental design. Biochemical and molecular characterization of the chitinase

2011 ◽  
Vol 62 (3) ◽  
pp. 1255-1268 ◽  
Author(s):  
Olfa Ghorbel-Bellaaj ◽  
Laila Manni ◽  
Kemel Jellouli ◽  
Noomen Hmidet ◽  
Moncef Nasri
Aquaculture ◽  
2020 ◽  
Vol 515 ◽  
pp. 734594 ◽  
Author(s):  
Sandeep Shankar Pattanaik ◽  
Paramita Banerjee Sawant ◽  
K.A. Martin Xavier ◽  
Kiran Dube ◽  
Prem Prakash Srivastava ◽  
...  

2021 ◽  
Vol 926 (1) ◽  
pp. 012069
Author(s):  
Nurhadini ◽  
W Yandi ◽  
M A Nugraha ◽  
M A Putri ◽  
N Riyani

Abstract The increasing production of shrimp commodities in Bangka Belitung Island can cause shrimp shell waste and polluting the environment. Shrimp shell waste can further proceed into chitosan which has a wide range of functions in various fields. This current study aims to find out the influence of the deacetylation process in chitosan extracts from shrimp shell waste. The method of chitosan extraction by varying repetition of deacetylation process. The characterization of chitosan extracts by FTIR analysis to determine functional group and degree of deacetylation (DD). Based on FTIR spectra, repetition in the deacetylation process in chitosan extraction still produces chitosan extracts that do not fully transform into chitosan. However, it able to increase DD with the highest DD of chitosan extract constitutes 86,78% and can be used in the further application.


2018 ◽  
Vol 17 (9) ◽  
pp. 446-453 ◽  
Author(s):  
Dina Sugiyanti ◽  
Purnama Darmadji ◽  
Sri Anggrahini ◽  
Chairil Anwar ◽  
Umar Santoso

1996 ◽  
Vol 44 (3) ◽  
pp. 682-685 ◽  
Author(s):  
Thomas A. Seymour ◽  
Shiao Jing Li ◽  
Michael T. Morrissey

Author(s):  
Vijayakumar Renuka ◽  
Chandragiri Nagaraja Rao Ravishankar ◽  
Krishnamoorthy Elavarasan ◽  
Abubacker Aliyamveetil Zynudheen ◽  
Toms C. Joseph

2006 ◽  
Vol 72 (12) ◽  
pp. 7879-7885 ◽  
Author(s):  
Jens Waldeck ◽  
Gabriele Daum ◽  
Bernward Bisping ◽  
Friedhelm Meinhardt

ABSTRACT Proteolytic but chitinase-deficient microbial cultures were isolated from shrimp shell waste and characterized. The most efficient isolate was found to be a mixed culture consisting of two Bacillus licheniformis strains, which were first determined microscopically and physiologically. Molecular characterization was carried out by sequencing the 16S rRNA gene of both strains. According to the residual protein and ash content, the chitin obtained by fermentation of such a mixed culture was found to be comparable to a commercially available, chemically processed product. However, the strikingly high viscosity (80 versus 10 mPa of the commercially available sample) indicates its superior quality. The two strains differed in colony morphology and in their secretion capabilities for degradative extracellular enzymes. Sequencing of the loci encoding amylase, cellulase, chitinases, and proteases, as well as the degS/degU operon, which is instrumental in the regulation of degradative enzymes, and the pga operon, which is responsible for polyglutamic acid production, revealed no differences. However, a frameshift mutation in chiA, encoding a chitinase, was validated for both strains, providing an explanation for the ascertained absence of chitinolytic activities and the concomitant possibility of producing highly viscous chitin in a fermentational deproteinization process.


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