Evaluation of genetic diversity of Chinese Pleurotus ostreatus cultivars using DNA sequencing technology

2012 ◽  
Vol 63 (2) ◽  
pp. 571-576 ◽  
Author(s):  
Yu Liu ◽  
Shouxian Wang ◽  
Yonggang Yin ◽  
Feng Xu
Keyword(s):  
Genetic Diversity ◽  
Dna Sequencing ◽  
Pleurotus Ostreatus ◽  
Sequencing Technology

Genetic diversity, core collection and breeding history of Pleurotus ostreatus in China

Mycoscience ◽  
2019 ◽  
Vol 60 (1) ◽  
pp. 14-24 ◽  
Author(s):  
Jing Li ◽  
Xiao-Bin Liu ◽  
Zhi-Wei Zhao ◽  
Zhu L. Yang
Keyword(s):  
Genetic Diversity ◽  
Pleurotus Ostreatus ◽  
Core Collection ◽  
History Of ◽  
Breeding History

Universal base analogues and their applications in DNA sequencing technology

RSC Advances ◽  
2013 ◽  
Vol 3 (35) ◽  
pp. 14910 ◽  
Author(s):  
Feng Liang ◽  
Ying-Zhu Liu ◽  
Peiming Zhang
Keyword(s):  
Dna Sequencing ◽  
Sequencing Technology

scholarly journals Microsatellite Markers for Aronia melanocarpa (Black Chokeberry) and Their Transferability to Other Aronia Species

HortScience ◽  
2017 ◽  
Vol 52 (1) ◽  
pp. 20-23
Author(s):  
Samuel G. Obae ◽  
Mark H. Brand ◽  
Bryan A. Connolly ◽  
Rochelle R. Beasley ◽  
Stacey L. Lance
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Microsatellite Markers ◽  
New Hampshire ◽  
Marker Assisted Selection ◽  
Genomic Sequencing ◽  
Sequencing Technology ◽  
Fruit Crop ◽  
Aronia Melanocarpa ◽  
Black Chokeberry

This study reports the development, characterization, and cross-species transferability of 20 genomic microsatellite markers for Aronia melanocarpa, an important nutraceutical fruit crop. The markers were developed with Illumina paired-end genomic sequencing technology using DNA from Professor Ed cultivar that was originally collected from the wild in New Hampshire. The markers were highly polymorphic and transferable to Aronia arbutifolia and Aronia prunifolia genomes. The average number of alleles per locus was 9.1, 4.5, and 5.6 for A. melanocarpa, A. arbutifolia, and A. prunifolia, respectively. The polymorphism information content (PIC) of loci ranged from 0.38 to 0.95 for all taxa, with an average of 0.80, 0.68, and 0.87 for A. melanocarpa, A. arbutifolia, and A. prunifolia, respectively. This is the first study to develop microsatellite markers in the Aronia genus. These markers will be very useful in studying the genetic diversity and population structure of wild Aronia and expediting the breeding efforts of this emerging fruit crop through marker-assisted selection.

scholarly journals Current and Future Methodology for Quantitation and Site-specific Mapping the Location of DNA Adducts

2021 ◽  
Author(s):  
Gunnar Boysen ◽  
Intawat Nookaew
Keyword(s):  
Dna Sequencing ◽  
Dna Adducts ◽  
Single Molecule ◽  
State Of The Art ◽  
Dna Adduct ◽  
Sequencing Technology ◽  
Specific Mass ◽  
Comprehensive Picture ◽  
Specific Mapping ◽  
Altered Gene

Abstract: Formation of DNA adducts is a key event for a genotoxic mode of action and its formation is often use as surrogate for mutation and cancer. Interest in DNA adducts are twofold, first, to demonstrate exposure, and second, to link DNA adduct location to subsequent mutations or altered gene regulation. High chemically specific mass spectrometry methods have been established for DNA adduct quantitation and elegant bio-analytic methods utilizing enzymes, various chemistries, and molecular biology methods to visualize the location of DNA adducts. Traditionally, these highly specific methods cannot be combined, and the results are incomparable. Initially developed for single-molecule DNA sequencing, nanopore-type technologies are expected to enable simultaneous quantitation and location of DNA adducts across the genome. We will briefly summarize the current methodologies for state-of-the-art quantitation of DNA adduct levels and mapping of DNA adducts and describe novel single-molecule DNA sequencing technology that is expected to achieve both measures simultaneously. Emerging technologies are expected to soon provide a comprehensive picture of the exposome and identify gene regions susceptible to DNA adduct formation.

scholarly journals Chemical and Genetic Diversity of Ligularia hodgsonii in China

2015 ◽  
Vol 10 (6) ◽  
pp. 1934578X1501000
Author(s):  
Chiaki Kuroda ◽  
Kou Inagaki ◽  
Xun Chao ◽  
Kyosuke Inoue ◽  
Yasuko Okamoto ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Dna Sequencing ◽  
Yunnan Province

Ligularia hodgsonii was found to be diverse in China. Furanoeremophilanes were isolated from samples collected in Yunnan Province, while such compounds were absent from samples from Sichuan, Gansu, and Chongqing. DNA sequencing showed that the Yunnan samples were also genetically distinct. γ-Humulene and a new bisabolane sesquiterpene were isolated.

Cyanobacterial biodiversity of semiarid public drinking water supply reservoirs assessed via next-generation DNA sequencing technology

2019 ◽  
Vol 57 (6) ◽  
pp. 450-460 ◽  
Author(s):  
Adriana Sturion Lorenzi ◽  
Mathias Ahii Chia ◽  
Fabyano Alvares Cardoso Lopes ◽  
Genivaldo Gueiros Z. Silva ◽  
Robert A. Edwards ◽  
...  
Keyword(s):  
Drinking Water ◽  
Water Supply ◽  
Dna Sequencing ◽  
Drinking Water Supply ◽  
Next Generation ◽  
Sequencing Technology ◽  
Next Generation Dna Sequencing ◽  
Public Drinking Water ◽  
Public Drinking ◽  
Water Supply Reservoirs

scholarly journals Cultivating DNA Sequencing Technology After the Human Genome Project

2020 ◽  
Vol 21 (1) ◽  
pp. 117-138
Author(s):  
Jeffery A. Schloss ◽  
Richard A. Gibbs ◽  
Vinod B. Makhijani ◽  
Andre Marziali
Keyword(s):  
Dna Sequencing ◽  
Human Genome ◽  
Human Genome Project ◽  
Technology Development ◽  
Genome Project ◽  
Alternative Methods ◽  
Biological Research ◽  
Sequencing Technology ◽  
Core Technology ◽  
The Human Genome Project

When the Human Genome Project was completed in 2003, automated Sanger DNA sequencing with fluorescent dye labels was the dominant technology. Several nascent alternative methods based on older ideas that had not been fully developed were the focus of technical researchers and companies. Funding agencies recognized the dynamic nature of technology development and that, beyond the Human Genome Project, there were growing opportunities to deploy DNA sequencing in biological research. Consequently, the National Human Genome Research Institute of the National Institutes of Health created a program—widely known as the Advanced Sequencing Technology Program—that stimulated all stages of development of new DNA sequencing methods, from innovation to advanced manufacturing and production testing, with the goal of reducing the cost of sequencing a human genome first to $100,000 and then to $1,000. The events of this period provide a powerful example of how judicious funding of academic and commercial partners can rapidly advance core technology developments that lead to profound advances across the scientific landscape.

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