RAPD PCR discloses high genetic heterogeneity among Vibrio parahaemolyticus from various environments along the southwest coast of India

2015 ◽  
Vol 66 (2) ◽  
pp. 925-929 ◽  
Author(s):  
Reshma Silvester ◽  
Deborah Alexander ◽  
Sudha Santha ◽  
Mohamed Hatha
Keyword(s):  
Genetic Heterogeneity ◽  
Vibrio Parahaemolyticus ◽  
Southwest Coast Of India ◽  
Southwest Coast ◽  
Rapd Pcr

Evaluation of RAPD-PCR and protein profile analysis to differentiate Vibrio harveyi strains prevalent along the southwest coast of India

2009 ◽  
Vol 88 (3) ◽  
pp. 273-279 ◽  
Author(s):  
Biswajit Maiti ◽  
Malathi Shekar ◽  
Rekha Khushiramani ◽  
Iddya Karunasagar ◽  
Indrani Karunasagar
Keyword(s):  
Vibrio Harveyi ◽  
Profile Analysis ◽  
Protein Profile ◽  
Southwest Coast Of India ◽  
Southwest Coast ◽  
Rapd Pcr

Detection and molecular characterization of Vibrio parahaemolyticus isolated from seafood harvested along the southwest coast of India

2008 ◽  
Vol 25 (6) ◽  
pp. 824-830 ◽  
Author(s):  
P RAGHUNATH ◽  
S ACHARYA ◽  
A BHANUMATHI ◽  
I KARUNASAGAR ◽  
I KARUNASAGAR
Keyword(s):  
Molecular Characterization ◽  
Vibrio Parahaemolyticus ◽  
Southwest Coast Of India ◽  
Southwest Coast

scholarly journals Seasonal Variation in Abundance of Total and Pathogenic Vibrio parahaemolyticus Bacteria in Oysters along the Southwest Coast of India

2005 ◽  
Vol 71 (7) ◽  
pp. 3575-3580 ◽  
Author(s):  
A. Deepanjali ◽  
H. Sanath Kumar ◽  
I. Karunasagar ◽  
I. Karunasagar
Keyword(s):  
Vibrio Parahaemolyticus ◽  
Pcr Amplification ◽  
Seasonal Abundance ◽  
Southwest Coast Of India ◽  
Colony Hybridization ◽  
Southwest Coast ◽  
Alkaline Peptone Water ◽  
Pathogenic Vibrio ◽  
Hemolysin Gene ◽  
Peptone Water

ABSTRACT The seasonal abundance of Vibrio parahaemolyticus in oysters from two estuaries along the southwest coast of India was studied by colony hybridization using nonradioactive labeled oligonucleotide probes. The density of total V. parahaemolyticus bacteria was determined using a probe binding to the tlh (thermolabile hemolysin) gene, and the density of pathogenic V. parahaemolyticus bacteria was determined by using a probe binding to the tdh (thermostable direct hemolysin) gene. Furthermore, the prevalence of V. parahaemolyticus was studied by PCR amplification of the toxR, tdh, and trh genes. PCR was performed directly with oyster homogenates and also following enrichment in alkaline peptone water for 6 and 18 h. V. parahaemolyticus was detected in 93.87% of the samples, and the densities ranged from <10 to 104 organisms per g. Pathogenic V. parahaemolyticus could be detected in 5 of 49 samples (10.2%) by colony hybridization using the tdh probe and in 3 of 49 samples (6.1%) by PCR. Isolates from one of the samples belonged to the pandemic serotype O3:K6. Twenty-nine of the 49 samples analyzed (59.3%) were positive as determined by PCR for the presence of the trh gene in the enrichment broth media. trh-positive V. parahaemolyticus was frequently found in oysters from India.

Characterization and distribution of microplastics in estuarine surface sediments, Kayamkulam estuary, southwest coast of India

2021 ◽  
Vol 168 ◽  
pp. 112389
Author(s):  
K. Radhakrishnan ◽  
V. Sivapriya ◽  
A. Rajkumar ◽  
N. Akramkhan ◽  
P. Prakasheswar ◽  
...  
Keyword(s):  
Surface Sediments ◽  
Southwest Coast Of India ◽  
Southwest Coast

scholarly journals Implications of an extensive salt water barrage on the distribution of black clam in a tropical estuarine system, Southwest coast of India

Oceanologia ◽  
2021 ◽  
Author(s):  
Arunpandi Nagarathinam ◽  
Jyothibabu Retnamma ◽  
Jagadeesan Loganathan ◽  
Parthasarathi Singaram ◽  
Savitha Mohanan ◽  
...  
Keyword(s):  
Salt Water ◽  
Estuarine System ◽  
Southwest Coast Of India ◽  
Southwest Coast ◽  
Tropical Estuarine
Sign in / Sign up

Export Citation Format

Share Document