Superior single nucleotide polymorphisms that contribute to two main routes of the fatty acid synthesis pathway in Korean cattle

Aim: The study aimed to identify fatty acid synthase (FASN), LOC514211, and fat mass and obesity-associated (FTO) gene polymorphisms and to investigate their associations with milk traits in an Indonesian-Holstein dairy cow population. Materials and Methods: A total of 100 Indonesian-Holstein cows consisting of 50 heads (0th generation; G0) and 50 heads of their daughters (1st generation; G1) were used. Polymerase chain reaction-restriction fragment length polymorphism was performed to genotype three single nucleotide polymorphisms: rs41919985 in the FASN gene, rs42688595 in the LOC514211 gene, and g.1371T>A in the FTO gene. Results: FASN rs41919985 was associated with milk protein percentage (p<0.05), FTO g.1371T>A was associated with milk fat percentage (p<0.05), and LOC514211 rs42688595 was not associated with any trait (p>0.05). Heterozygote variants showed a higher protein percentage for FASN and the highest fat percentage for FTO. These associations were consistent in the G0 and G1 populations. Conclusion: Our results indicate that the milk protein and fat percentages can be improved by increasing the frequency of the AG genotype of FASN and the AT genotype of FTO, respectively.

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Single Nucleotide Polymorphisms in the FADS Gene Cluster but not the ELOVL2 Gene are Associated with Serum Polyunsaturated Fatty Acid Composition and Development of Allergy (in a Swedish Birth Cohort)

Nutrients ◽

10.3390/nu7125521 ◽

2015 ◽

Vol 7 (12) ◽

pp. 10100-10115 ◽

Cited By ~ 13

Author(s):

Malin Barman ◽

Staffan Nilsson ◽

Åsa Torinsson Naluai ◽

Anna Sandin ◽

Agnes Wold ◽

...

Keyword(s):

Fatty Acid Composition ◽

Fatty Acid ◽

Single Nucleotide Polymorphisms ◽

Gene Cluster ◽

Birth Cohort ◽

Acid Composition ◽

Polyunsaturated Fatty Acid ◽

Nucleotide Polymorphisms ◽

Single Nucleotide

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Thailandamide, a Fatty Acid Synthesis Antibiotic That Is Coexpressed with a Resistant Target Gene

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.00463-18 ◽

2018 ◽

Vol 62 (9) ◽

Cited By ~ 6

Author(s):

Christopher E. Wozniak ◽

Zhenjian Lin ◽

Eric W. Schmidt ◽

Kelly T. Hughes ◽

Theodore G. Liou

Keyword(s):

Fatty Acid ◽

Fatty Acid Synthesis ◽

Target Gene ◽

Gene Clusters ◽

Acid Synthesis ◽

Inducible Promoter ◽

Content Type ◽

Uncharacterized Gene ◽

Fatty Acid Synthesis Pathway ◽

Genetic Techniques

ABSTRACTMicrobes encode many uncharacterized gene clusters that may produce antibiotics and other bioactive small molecules. Methods for activating these genes are needed to explore their biosynthetic potential. A transposon containing an inducible promoter was randomly inserted into the genome of the soil bacteriumBurkholderia thailandensisto induce antibiotic expression. This screen identified the polyketide/nonribosomal peptide thailandamide as an antibiotic and discovered its regulator, AtsR. Mutants ofSalmonellaresistant to thailandamide had mutations in theaccAgene for acetyl coenzyme A (acetyl-CoA) carboxylase, which is one of the first enzymes in the fatty acid synthesis pathway. A second copy ofaccAin the thailandamide synthesis gene cluster keepsB. thailandensisresistant to its own antibiotic. These genetic techniques will likely be powerful tools for discovering other unusual antibiotics.

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