scholarly journals Patulin Imprinted Nanoparticles Decorated Surface Plasmon Resonance Chips for Patulin Detection

2021 ◽  
Author(s):  
Duygu Çimen ◽  
Nilay Bereli ◽  
Adil Denizli
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Limit Of Detection ◽  
Kinetic Studies ◽  
Size Analysis ◽  
Limit Of Quantification ◽  
Two Phase ◽  
Angle Measurements ◽  
Liquid Chromatography Tandem Mass

AbstractIn this study, the patulin imprinted and the non-imprinted nanoparticles are synthesized by the two-phase mini emulsion polymerization method and characterized by zeta-size analysis, Fourier transform infrared spectroscopy, and scanning electron microscopy. Afterwards, the patulin imprinted and the non-imprinted nanoparticles are attached on the surface of surface plasmon resonance (SPR) chips. The patulin imprinted and the non-imprinted SPR nanosensors are characterized by using atomic force microscope, ellipsometer, and contact angle measurements. Kinetic studies for patulin detection are carried out in the concentration range of 0.5 nmolar–750 nmolar. The limit of detection and the limit of quantification values are obtained as 0.011 nmolar and 0.036 nmolar, respectively. In all kinetic analysis, the response time is 13 min for equilibration, adsorption, and desorption cycles. The selectivity studies of the patulin imprinted and the non-imprinted SPR nanosensors are determined in the presence of ochratoxin A and aflatoxin B1. In order to demonstrate the applicability, validation studies of the patulin imprinted SPR nanosensor are performed by liquid chromatography-tandem mass spectrometry (LC-MS).

scholarly journals Surface Plasmon Resonance Based on Molecularly Imprinted Polymeric Film for l-Phenylalanine Detection

Biosensors ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 21
Author(s):  
Duygu Çimen ◽  
Nilay Bereli ◽  
Adil Denizli
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Limit Of Detection ◽  
Kinetic Studies ◽  
Force Microscopy ◽  
Spr Sensor ◽  
Atomic Force ◽  
Analysis Technique ◽  
Molecular Imprinting Technology

In this study, we designed a simple, rapid, sensitive and selective surface plasmon resonance (SPR) sensor for detection of L-phenylalaine by utilizing molecular imprinting technology. l-phenylalanine imprinted and non-imprinted poly(2-hydroxyethyl methacrylate-methacryloyl-l-phenylalanine) polymeric films were synthesized onto SPR chip surfaces using ultraviolet polymerization. l-phenyalanine imprinted and non-imprinted SPR sensors were characterized by using contact angle, atomic force microscopy and ellipsometry. After characterization studies, kinetic studies were carried out in the concentration range of 5.0–400.0 μM. The limit of detection and quantification were obtained as 0.0085 and 0.0285 μM, respectively. The response time for the test including equilibration, adsorption and desorption was approximately 9 min. The selectivity studies of the l-phenylalanine imprinted SPR sensor was performed in the presence of d-phenylalanine and l-tryptophan. Validation studies were carried out via enzyme-linked immunosorbent analysis technique in order to demonstrate the applicability and superiority of the l-phenylalanine imprinted SPR sensor.

Surface Plasmon Resonance Based Fiber Optic Dopamine Sensor Using BSA-Gold Cluster/Polymer Composites

2018 ◽  
Vol 24 (8) ◽  
pp. 5598-5602 ◽  
Author(s):  
D. Rithesh Raj ◽  
C Sudarsanakumar
Keyword(s):  
Surface Plasmon Resonance ◽  
Polymer Composites ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Fiber Optic ◽  
Limit Of Detection ◽  
Gold Cluster ◽  
Gold Clusters ◽  
Fiber Optic Sensor ◽  
Size Analysis

Highly luminescent BSA-gold clusters were synthesized and its polymer composites were prepared using PVA, PVP and PVA/PVP blend. UV-visible spectroscopy, Photoluminescence, Fourier transform infrared spectroscopy (FTIR), Transmission electron microscopy (TEM) and particle size analysis (DLS) were performed to determine the formation of gold clusters. Luminescence properties of BSA-Gold cluster and its polymer composites were evaluated and it is found that all polymers quench the fluorescence at 650 nm while PVP and PVP/PVA blend enhance the fluorescence at 450 nm. Further, a Surface Plasmon Resonance (SPR) based fiber optic sensor has been fabricated using PVP/BSA-gold clusters as the sensing material and its dopamine sensing properties were studied. The sensor shows a linear response in the range of 0 to 80 μM concentrations of dopamine and the limit of detection (LOD) of the sensor is 12 μM.

Development of a Surface Plasmon Resonance–Based Immunoassay for Listeria monocytogenes

2005 ◽  
Vol 68 (4) ◽  
pp. 728-735 ◽  
Author(s):  
PAUL LEONARD ◽  
STEPHEN HEARTY ◽  
GARY WYATT ◽  
JOHN QUINN ◽  
RICHARD O'KENNEDY
Keyword(s):  
Surface Plasmon Resonance ◽  
Listeria Monocytogenes ◽  
Surface Plasmon ◽  
Polyclonal Antibody ◽  
Plasmon Resonance ◽  
Limit Of Detection ◽  
Chip Surface ◽  
Metal Affinity ◽  
Coefficients Of Variation ◽  
Internalin B

A polyclonal antibody was produced against Internalin B (InlB)–enriched extract and used to develop an inhibition assay to detect Listeria monocytogenes cells in solution using surface plasmon resonance. The gene sequence encoding for the InlB protein was cloned into a Qiagen pQE-60 vector, expressed in Escherichia coli, and purified by immobilized metal affinity chromatography. Protein G–purified anti-InlB–enriched extract polyclonal antibody was incubated with various concentrations of L. monocytogenes cells and subsequently injected over a purified-recombinant InlB (rInlB)–immobilized CM5 sensor chip surface. A decrease in antibody binding response was observed with increasing L. monocytogenes cell concentrations. Intraday and interday assay variability studies were carried out to evaluate precision and reproducibility. The assay had a limit of detection of less than 2 × 105 cells per ml and could be successfully reproduced with coefficients of variation of between 2.5 and 7.7%.

scholarly journals A Surface Plasmon Resonance Plastic Optical Fiber Biosensor for the Detection of Pancreatic Amylase in Surgically-Placed Drain Effluent

Sensors ◽  
2021 ◽  
Vol 21 (10) ◽  
pp. 3443
Author(s):  
Laura Pasquardini ◽  
Nunzio Cennamo ◽  
Giuseppe Malleo ◽  
Lia Vanzetti ◽  
Luigi Zeni ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Optical Fiber ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Limit Of Detection ◽  
Optical Biosensor ◽  
Plastic Optical Fiber ◽  
Epithelial Surface ◽  
Cheap Method ◽  
Log Linear

Postoperative pancreatic fistula (POPF), the major driver of morbidity and mortality following pancreatectomy, is caused by an abnormal communication between the pancreatic ductal epithelium and another epithelial surface containing pancreas-derived, enzyme-rich fluid. There is a strong correlation between the amylase content in surgically-placed drains early in the postoperative course and the development of POPF. A simple and cheap method to determine the amylase content from the drain effluent has been eagerly advocated. Here, we developed an amylase optical biosensor, based on a surface plasmon resonance (SPR) plastic optical fiber (POF), metallized with a 60 nm layer of gold and interrogated with white light. The sensor was made specific by coupling it with an anti-amylase antibody. Each surface derivatization step was optimized and studied by XPS, contact angle, and fluorescence. The POF-biosensor was tested for its response to amylase in diluted drain effluents. The volume of sample required was 50 µL and the measurement time was 8 min. The POF-biosensor showed selectivity for amylase, a calibration curve log-linear in the range of 0.8–25.8 U/L and a limit of detection (LOD) of ~0.5 U/L. In preliminary tests, the POF-biosensor allowed for the measurement of the amylase content of diluted surgically-placed drain effluents with an accuracy of >92% with respect to the gold standard. The POF-biosensor allows for reliable measurement and could be implemented to allow for a rapid bedside assessment of amylase value in drains following pancreatectomy.

scholarly journals Development of Rapid, Sensitive, and Effective Plasmonic Nanosensor for the Detection of Vitamins in Infact Formula and Milk Samples

2020 ◽  
Vol 10 (4) ◽  
pp. 316-332 ◽  
Author(s):  
Duygu Çimen ◽  
Adil Denizli
Keyword(s):  
Surface Plasmon Resonance ◽  
Vitamin B12 ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Limit Of Detection ◽  
Vitamin B1 ◽  
Surface Plasmon Resonance Sensor ◽  
Vitamin B2 ◽  
Vitamin B9 ◽  
Resonance Sensor

Abstract The aim of the present study is to develop a surface plasmon resonance sensor for the detection of vitamin B2, vitamin B9, and vitamin B12 in food samples by using the molecular imprinting technique. The vitamin B2, vitamin B9, and vitamin B12 imprinted and the non-imprinted surface plasmon resonance sensor chip surfaces were characterized by using contact angle measurements, atomic force microscopy, ellipsometry, and Fourier transform infrared-attenuated total reflectance. The real-time detection of vitamin B2, vitamin B9, and vitamin B12 was analyzed by using aqueous solutions in the concentration range of 0.01 ng/mL − 10 ng/mL for vitamin B2, 0.1 ng/mL − 8.0 ng/mL for vitamin B9, and 0.01 ng/mL − 1.5 ng/mL for vitamin B12. The limit of detection values was calculated as 1.6×10−4 ng/mL for vitamin B2, 13.5×10−4 ng/mL for vitamin B9, and 2.5×10−4 ng/mL for vitamin B12, respectively. Selectivity experiments were performed by using vitamin B1 and vitamin B6. The reproducibility of surface plasmon resonance sensors was investigated both on the same day and on different days for four times. Validation studies of the prepared surface plasmon resonance (SPR) sensors were performed by liquid chromatography-tandem mass spectrometry (LC-MS/MS).

scholarly journals Label-Free Direct Detection of Saxitoxin Based on a Localized Surface Plasmon Resonance Aptasensor

Toxins ◽  
2019 ◽  
Vol 11 (5) ◽  
pp. 274 ◽  
Author(s):  
Su-Ji Ha ◽  
Jin-Ho Park ◽  
Bobin Lee ◽  
Min-Gon Kim
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Localized Surface Plasmon Resonance ◽  
Limit Of Detection ◽  
Direct Detection ◽  
Localized Surface Plasmon ◽  
Label Free ◽  
Health Food ◽  
Detection Range

Seafood is an emerging health food, and interest in improving the quality of seafood is increasing. Saxitoxin (STX) is a neurotoxin produced by marine dinoflagellates that is accumulated in seafood. It can block the neuronal transmission between nerves and muscle cell membranes, resulting in the disturbance of neuromuscular transmission and subsequent voluntary muscle paralysis. Here, we developed a new aptamer for the detection of STX using graphene oxide–systematic evolution of ligands by exponential enrichment (GO-SELEX). Furthermore, we confirmed sensitivity and selectivity of the developed aptamer specific to STX using a localized surface plasmon resonance (LSPR) sensor. The sensing chip was fabricated by fixing the new STX aptamer immobilized on the gold nanorod (GNR) substrate. The STX LSPR aptasensor showed a broad, linear detection range from 5 to 10,000 μg/L, with a limit of detection (LOD) of 2.46 μg/L (3σ). Moreover, it was suitable for the detection of STX (10, 100, and 2000 μg/L) in spiked mussel samples and showed a good recovery rate (96.13–116.05%). The results demonstrated that the new STX aptamer-modified GNR chip was sufficiently sensitive and selective to detect STX and can be applied to real samples as well. This LSPR aptasensor is a simple, label-free, cost-effective sensing system with a wide detectable range.

scholarly journals Rapid and Sensitive Detection of Lung Cancer Biomarker Using Nanoporous Biosensor Based on Localized Surface Plasmon Resonance Coupled with Interferometry

2015 ◽  
Vol 2015 ◽  
pp. 1-11 ◽  
Author(s):  
Jae-Sung Lee ◽  
Sae-Wan Kim ◽  
Eun-Yoon Jang ◽  
Byoung-Ho Kang ◽  
Sang-Won Lee ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Localized Surface Plasmon Resonance ◽  
Limit Of Detection ◽  
Surrounding Medium ◽  
Electrochemical Anodization ◽  
Localized Surface Plasmon ◽  
Label Free Detection

We propose a nanobiosensor to evaluate a lung cancer-specific biomarker. The nanobiosensor is based on an anodic aluminum oxide (AAO) chip and functions on the principles of localized surface plasmon resonance (LSPR) and interferometry. The pore-depth of the fabricated nanoporous AAO chip was 1 µm and was obtained using a two-step electrochemical anodization process. The sensor chip is sensitive to the refractive index (RI) changes of the surrounding medium and also provides simple and label-free detection when specific antibodies are immobilized on the gold-deposited surface of the AAO chip. In order to confirm the effectiveness of the sensor, the antibodies were immobilized on the surface of the AAO chip, and the lung cancer-specific biomarker was applied atop of the immobilized-antibody layer using the self-assembled monolayer method. The nanoporous AAO chip was used as a sensor system to detect serum amyloid A1, which is a lung cancer-specific biomarker. The specific reaction of the antigen-antibody contributes to the change in the RI. This in turn causes a shift in the resonance spectrum in the refractive interference pattern. The limit of detection (LOD) was found to be 100 ag/mL and the biosensor had high sensitivity over a wide concentration range.

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