Association of a monopartite begomovirus and associated betasatellite with yellow vein disease of a weed host, Senna italica Mill. In Oman

VirusDisease ◽  
2021 ◽  
Author(s):  
Salma Saif Al-Mabsli ◽  
Ali K. Al-Wahaibi ◽  
A. M. Al-Sadi ◽  
M. S. Shahid
Keyword(s):  
Yellow Vein ◽  
Monopartite Begomovirus ◽  
Weed Host ◽  
Vein Disease

scholarly journals Association of a Monopartite Begomovirus Producing Subgenomic DNA and a Distinct DNA Beta on Croton bonplandianus Showing Yellow Vein Symptoms in Pakistan

Plant Disease ◽  
2002 ◽  
Vol 86 (4) ◽  
pp. 444-444 ◽  
Author(s):  
I. Amin ◽  
S. Mansoor ◽  
S. Iram ◽  
M. A. Khan ◽  
M. Hussain ◽  
...  
Keyword(s):  
Monopartite Begomoviruses ◽  
Full Length ◽  
Yellow Vein ◽  
Bipartite Begomoviruses ◽  
Universal Primers ◽  
Cotton Leaf ◽  
Nylon Membrane ◽  
Monopartite Begomovirus ◽  
Punjab Province ◽  
Vein Disease

The recent discovery that monopartite begomoviruses on ageratum and cotton essentially require a DNA satellite called DNA β (2,4) is leading to identification of several other hosts that have similar disease complexes. A weed species (Croton bonplandianus) belonging to the family Euphorbiaceae is one such example. C. bonplandianus is widely distributed on wastelands throughout the Punjab Province in Pakistan. It very often shows yellow vein symptoms indicating infection by a begomovirus. To detect a begomovirus, both symptomatic and asymptomatic plants were collected from several widely separated locations in the Punjab Province. Total DNA was isolated from these samples by the cetyltrimethylammoniumbromide (CTAB) method, resolved in an agarose gel, and blotted on a nylon membrane (2). A full-length clone of DNA A of Cotton leaf curl virus (CLCuV) labeled with 32PdCTP was used as a probe in Southern hybridization (2). The probe detected hybridizing bands only in symptomatic plants, confirming the presence of a begomovirus. In addition to hybridizing bands of the expected sizes, smaller bands were also detected, suggesting the presence of subgenomic molecules derived from DNA A. Universal polymerase chain reaction (PCR) primers for dicot-infecting geminiviruses (1) were used in PCR for amplification of DNA A of the begomovirus associated with the disease. The use of these primers in PCR was expected to result in amplification of full-length DNA A. In addition to a product of the expected size (2.7 to 2.8 kb), another product of approximately 1.4 kb was amplified. The presence of subgenomic DNAs that are derived from DNA A is an indicator of the monopartite nature of begomoviruses, because in bipartite begomoviruses subgenomic DNAs are derived solely from DNA B. The presence of a DNA β, a DNA satellite associated with certain monopartite begomoviruses, was suspected because of symptoms and the possible monopartite nature of the virus. Universal primers for amplification of DNA β (3) were used in PCR for amplification of a putative DNA β. The PCR reaction yielded a product of expected size (≈1.4 kb). A probe from the amplified product was made by the oligolabeling method. The probe detected hybridizing bands in all symptomatic samples collected from three locations, confirming the association of a DNA β with the disease. A duplicate blot when hybridized with a DNA β associated with ageratum yellow vein disease did not hybridize to these samples. These results confirm that yellow vein disease on this weed is associated with a monopartite begomovirus and a distinct DNA β. References: (1) R. W. Briddon et al. Mol. Biotechnol. 1:202, 1994. (2) R. W. Briddon et al. Virology 285:234, 2001. (3) R. W. Briddon et al. Mol. Biotechnol. In press. (4) K. Saunders et al. Proc. Natl. Acad. Sci. U S A 97:6890, 2000.

scholarly journals Epidemiology of Blackberry yellow vein associated virus

Plant Disease ◽  
2013 ◽  
Vol 97 (10) ◽  
pp. 1352-1357 ◽  
Author(s):  
Bindu Poudel ◽  
William M. Wintermantel ◽  
Arturo A. Cortez ◽  
Thien Ho ◽  
Archana Khadgi ◽  
...  
Keyword(s):  
The United States ◽  
Yellow Vein ◽  
Chain Reaction ◽  
Efficient Detection ◽  
Blackberry Yellow Vein Disease ◽  
Polymerase Chain ◽  
Multiple Levels ◽  
And Control ◽  
Trialeurodes Abutilonea ◽  
Vein Disease

Blackberry yellow vein disease is one of the most important diseases of blackberry in the United States. Several viruses are found associated with the symptomology but Blackberry yellow vein associated virus (BYVaV) appears to be the most prevalent of all, leading to the need for a better understanding of its epidemiology. Efficient detection protocols were developed using end-point and quantitative reverse-transcription polymerase chain reaction. A multi-state survey was performed on wild and cultivated blackberry to assess the geographical distribution of the virus. Two whitefly species, Trialeurodes abutilonea and T. vaporariorum, were identified as vectors and 25 plant species were tested as potential BYVaV hosts. The information obtained in this study can be used at multiple levels to better understand and control blackberry yellow vein disease.

Economic injury levels for the potato yellow vein disease and its vector, Trialeurodes vaporariorum (Hemiptera: Aleyrodidae), affecting potato crops in the Andes

2019 ◽  
Vol 119 ◽  
pp. 52-58 ◽  
Author(s):  
Diego F. Rincon ◽  
Diego F. Vasquez ◽  
Hugo Fernando Rivera-Trujillo ◽  
Carlos Beltrán ◽  
Felipe Borrero-Echeverry
Keyword(s):  
Trialeurodes Vaporariorum ◽  
Yellow Vein ◽  
The Andes ◽  
Economic Injury ◽  
Potato Crops ◽  
Vein Disease

Associations of papaya leaf curl virus and betasatellite with yellow vein disease of pot marigold (Calendula officinalis L.)

2019 ◽  
Vol 52 (19-20) ◽  
pp. 1289-1297
Author(s):  
V. Venkataravanappa ◽  
Priti Sonavane ◽  
C. N. Lakshminarayana Reddy ◽  
M. Krishna Reddy
Keyword(s):  
Yellow Vein ◽  
Calendula Officinalis ◽  
Pot Marigold ◽  
Calendula Officinalis L ◽  
Leaf Curl Virus ◽  
Vein Disease ◽  
Papaya Leaf Curl Virus ◽  
Leaf Curl

scholarly journals The DNA β satellite component associated with ageratum yellow vein disease encodes an essential pathogenicity protein (βC1)

Virology ◽  
2004 ◽  
Vol 324 (1) ◽  
pp. 37-47 ◽  
Author(s):  
Keith Saunders ◽  
Alexandra Norman ◽  
Sebastien Gucciardo ◽  
John Stanley
Keyword(s):  
Yellow Vein ◽  
Vein Disease

scholarly journals New natural weed host Raphanus raphanistrum L. (Brassicaceae) for Beet necrotic yellow vein virus and its vector Polymyxa betae Keskin

2016 ◽  
Vol 40 ◽  
pp. 120-126 ◽  
Author(s):  
Nazlı Dide KUTLUK YILMAZ ◽  
Emine KAYA ALTOP ◽  
Colin James PHILLIPPO ◽  
Hüsrev MENNAN
Keyword(s):  
Yellow Vein ◽  
Polymyxa Betae ◽  
Raphanus Raphanistrum ◽  
Weed Host

Molecular identification of a begomovirus causing yellow vein disease on Calendula officinalis in India

EPPO Bulletin ◽  
2007 ◽  
Vol 37 (2) ◽  
pp. 420-426 ◽  
Author(s):  
A. A. Khan ◽  
M. S. Khan ◽  
S. K. Raj ◽  
Q. A. Naqvi
Keyword(s):  
Molecular Identification ◽  
Yellow Vein ◽  
Calendula Officinalis ◽  
Vein Disease

Complete genome sequence of a previously undescribed monopartite begomovirus and betasatellite infecting Malvastrum coromandelianum in Cambodia

2021 ◽  
Author(s):  
Yafei Tang ◽  
Zhenggang Li ◽  
Xiaoman She ◽  
Lin Yu ◽  
Guobing Lan ◽  
...  
Keyword(s):  
Rolling Circle Amplification ◽  
Pcr Amplification ◽  
Monopartite Begomoviruses ◽  
Yellow Vein ◽  
Structure Characteristic ◽  
Restriction Enzyme Digestion ◽  
Monopartite Begomovirus ◽  
Rolling Circle ◽  
Ageratum Yellow Vein Virus ◽  
Malvastrum Coromandelianum

Abstract A previously undescribed monopartite begomovirus was identified from Malvastrum coromandelianum plants exhibiting yellow vein symptoms characteristic of begomoviruses, in Kampot province, Cambodia. The apparently full-length viral component was cloned and sequenced following enrichment of circular DNA by rolling circle amplification and restriction enzyme digestion. The genome of the virus was 2,737 nucleotides in length (KP188831), and exhibited an organization like that of other monopartite begomoviruses, sharing the highest nt identities of 87.7% with Ageratum yellow vein virus (AM940137). A satellite molecule was amplified from total DNA by PCR amplification with the betasatellite-specific primer pair β01/β02. The satellite molecule (1,346 nt, KP188832) had a structure characteristic like other betasatellites associated with begomoviruses, and shared the highest nt identity of 84.8% with Malvastrum yellow vein betasatellite (MN205547). According to the criteria established for species demarcation for classification of begomoviruses ( Geminiviridae ) and betasatellites ( Tolecusatellitidae ), respectively, the virus isolate from M. coromandelianum in Cambodia is a previously undescribed novel monopartite begomovirus species，for which the name Malvastrum yellow vein Cambodia virus (MaYVCV) is proposed, whereas, the betasatellite is identified as an previously undescribed novel betasatellite species, for which the name Malvastrum yellow vein Cambodia batesatellite (MaYVKHB) is proposed.

scholarly journals A unique virus complex causes Ageratum yellow vein disease

2000 ◽  
Vol 97 (12) ◽  
pp. 6890-6895 ◽  
Author(s):  
K. Saunders ◽  
I. D. Bedford ◽  
R. W. Briddon ◽  
P. G. Markham ◽  
S. M. Wong ◽  
...  
Keyword(s):  
Yellow Vein ◽  
Virus Complex ◽  
Vein Disease
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