Enzymatic Synthesis of Mono- and Diglyceride Using Lipase From Candida rugosa Immobilized Onto Cellulose Acetate-Coated Fe2O3 Nanoparticles

2016 ◽  
Vol 41 (7) ◽  
pp. 2553-2561 ◽  
Author(s):  
Abhishek Kumar Singh ◽  
Mausumi Mukhopadhyay
2008 ◽  
Vol 50 (1) ◽  
pp. 33-39 ◽  
Author(s):  
Mohd. Basyaruddin Abdul Rahman ◽  
Uswatun Hasanah Zaidan ◽  
Mahiran Basri ◽  
Mohd. Zobir Hussein ◽  
Raja Noor Zaliha Raja Abd. Rahman ◽  
...  

2013 ◽  
Vol 78 (10) ◽  
pp. 1491-1501
Author(s):  
Nevena Prlainovic ◽  
Dejan Bezbradica ◽  
Zorica Knezevic-Jugovic ◽  
Dusan Velickovic ◽  
Dusan Mijin

3-Cyano-4-ethoxymethyl-6-methyl-2-pyridone is an important precursor in the synthesis of vitamin B6, obtained in the addition reaction between 2-cyanoacetamide and 1-ethoxy-2,4-pentanedione catalyzed by lipase from Candida rugosa (triacylglycerol ester hydrolases, EC 3.1.1.3). This work shows new experimental data and mathematical modeling of lipase catalyzed synthesis of 3-cyano-4-ethoxymethyl-6-methyl-2-pyridone, starting from 1-ethoxy-2,4-pentanedione and 2-cyanoacetamide. Kinetic measurements were done at 50 oC with enzyme concentration of 1.2 % w/v. Experimental results were fitted with two kinetic models: the ordered bi-ter and ping-pong bi-ter model, and the initial rates of the reaction were found to correlate best with a ping-pong bi-ter mechanism with inhibition by 2-cyanoacetamide. Obtained specificity constants indicated that lipase from C. rugosa had higher affinity towards 1-ethoxy-2,4-pentanedione and less bulky substrates.


2011 ◽  
Vol 34 (7) ◽  
pp. 803-810 ◽  
Author(s):  
Nevena Ž. Prlainović ◽  
Zorica D. Knežević-Jugović ◽  
Dušan Ž. Mijin ◽  
Dejan I. Bezbradica

2021 ◽  
Vol 362 ◽  
pp. 122-129 ◽  
Author(s):  
Keysson V. Fernandes ◽  
Elisa D.C. Cavalcanti ◽  
Eliane P. Cipolatti ◽  
Erika C.G. Aguieiras ◽  
Martina C.C. Pinto ◽  
...  

2005 ◽  
Vol 29 (2) ◽  
pp. 111-116 ◽  
Author(s):  
Mohd Basyaruddin Abdul Rahman ◽  
Safarini Md Tajudin ◽  
Mohd Zobir Hussein ◽  
Raja Nor Zaliha Raja Abdul Rahman ◽  
Abu Bakar Salleh ◽  
...  

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