Polymeric Nanoparticles Decorated with Monoclonal Antibodies: A New Immobilization Strategy for Increasing Lipase Activity

Recent advances in nanotechnology techniques enable the production of polymeric nanoparticles with specific morphologies and dimensions and, by tailoring their surfaces, one can manipulate their characteristics to suit specific applications. In this work we report an innovative approach for the immobilization of a commercial lipase from Candida rugosa (CRL) which employs nanostructured polymeric carriers conjugated with anti-lipase monoclonal antibodies (MoAbs). MoAbs were chemically conjugated on the surface of polymeric nanoparticles and used to selectively adsorb CRL molecules. Hydrolytic enzymatic assays evidenced that such immobilization technique afforded a significant enhancement of enzymatic activity in comparison to the free enzyme.

Synthesis of paramagnetic dendritic silica nanomaterials with fibrous pore structure (Fe3O4@KCC-1) and their application in immobilization of lipase from Candida rugosa with enhanced catalytic activity and stability

Paramagnetic mesoporous fibrous silica (Fe3O4@KCC-1) was prepared and its surface was functionalized with 3-aminopropyltriethoxysilane (APTES).

A Study on the Lipase-catalysed Acylation of 6,7-Dihydroxy-linalool

We here describe a study of the enzyme-mediated acylation reaction of 6,7-dihydroxy-linalool stereoisomers, which are natural triols occurring in different vegetal species. We found that only few lipases are able to catalyze the acylation of the secondary hydroxy group present in these isomers and only lipase from Candida rugosa and novozyme 435 provide either (3 R,6 R)-6-acetoxy-7-hydroxylinalool or (3 R,6 S)-6-acetoxy-7-hydroxylinalool in moderate and very good isomeric purity, respectively Even for these favorable cases the reaction proceeds very sluggishly. Our finding can give a sensible interpretation to the fact that 6-acyl-7-hydroxy-linalool derivatives do not occur in nature, whereas the corresponding glycosides, whose formation is catalyzed by glycosidase, are very common.