Establishment and characterization of NCC-MFS3-C1: a novel patient-derived cell line of myxofibrosarcoma

Human Cell ◽  
2021 ◽  
Author(s):  
Ryuto Tsuchiya ◽  
Yuki Yoshimatsu ◽  
Rei Noguchi ◽  
Yooksil Sin ◽  
Takuya Ono ◽  
...  
Keyword(s):  
Cell Line

Characterization of oxytocin receptor in a human granulosa cell line

1998 ◽  
Vol 5 (1) ◽  
pp. 106A-106A
Author(s):  
J COPLAND ◽  
M ZLATMK ◽  
K IVES ◽  
M SOLOFF
Keyword(s):  
Cell Line ◽  
Granulosa Cell ◽  
Oxytocin Receptor ◽  
Human Granulosa Cell

Establishment and Characterization of a Human Glioblastoma Cell Line Which Expresses Somatostatin and its Functional Receptor

1999 ◽  
Vol 11 (3) ◽  
pp. 215-222
Author(s):  
Nobuo HIROTA ◽  
Kiyoshi MATSUMOTO ◽  
Shigeki SUNAGA ◽  
Masataka IIDA ◽  
Hiroshi SAKAGAMI ◽  
...  
Keyword(s):  
Cell Line ◽  
Glioblastoma Cell Line ◽  
Glioblastoma Cell ◽  
Human Glioblastoma ◽  
Human Glioblastoma Cell Line ◽  
Human Glioblastoma Cell ◽  
Functional Receptor

Characterization of a bovine intestinal myofibroblast cell line and stimulation using phytoglycogen-based nanoparticles bound to inosine monophosphate

2021 ◽  
Vol 57 (1) ◽  
pp. 86-94
Author(s):  
K. Jenik ◽  
T. N. Alkie ◽  
E. Moore ◽  
J. D. Dejong ◽  
L. E. J. Lee ◽  
...  
Keyword(s):  
Cell Line ◽  
Inosine Monophosphate

Establishment and characterization of NCC-ssRMS2-C1: a novel patient-derived cell line of spindle cell/sclerosing rhabdomyosarcoma

Human Cell ◽  
2021 ◽  
Author(s):  
Ryuto Tsuchiya ◽  
Yuki Yoshimatsu ◽  
Rei Noguchi ◽  
Yooksil Sin ◽  
Takuya Ono ◽  
...  
Keyword(s):  
Cell Line ◽  
Spindle Cell ◽  
Sclerosing Rhabdomyosarcoma

Establishment and characterization of a novel cell line, NCC-DDLPS2-C1, derived from a patient with dedifferentiated liposarcoma

Human Cell ◽  
2021 ◽  
Author(s):  
Rei Noguchi ◽  
Yuki Yoshimatsu ◽  
Takuya Ono ◽  
Akane Sei ◽  
Kaoru Hirabayashi ◽  
...  
Keyword(s):  
Cell Line ◽  
Dedifferentiated Liposarcoma

scholarly journals Real-Time Challenging of ERα Y537S Mutant Transcriptional Activity in Living Cells

Endocrines ◽  
2021 ◽  
Vol 2 (1) ◽  
pp. 54-64
Author(s):  
Manuela Cipolletti ◽  
Sara Pescatori ◽  
Filippo Acconcia
Keyword(s):  
Cell Line ◽  
Transcriptional Activity ◽  
Estrogen Receptor Α ◽  
Living Cells ◽  
Patient Treatment ◽  
Estrogen Responsive Element ◽  
Specific Proteins ◽  
Responsive Element ◽  
Mcf 7

Metastatic estrogen receptor α (ERα)-expressing breast cancer (BC) occurs after prolonged patient treatment with endocrine therapy (ET) (e.g., aromatase inhibitors—AI; 4OH-tamoxifen—4OH-Tam). Often these metastatic BCs express a mutated ERα variant (e.g., Y537S), which is transcriptionally hyperactive, sustains uncontrolled proliferation, and renders tumor cells insensitive to ET drugs. Therefore, new molecules blocking hyperactive Y537S ERα mutation transcriptional activity are requested. Here we generated an MCF-7 cell line expressing the Y537S ERα mutation stably expressing an estrogen-responsive element (ERE) promoter, which activity can be monitored in living cells. Characterization of this cell line shows both hyperactive basal transcriptional activity with respect to normal MCF-7 cells, which stably express the same ERE-based promoter and a decreased effect of selective ER downregulators (SERDs) in reducing Y537S ERα mutant transcriptional activity with respect to wild type ERα transcriptional activity. Kinetic profiles of Y537S ERα mutant-based transcription produced by both drugs inducing receptor degradation and siRNA-mediated depletion of specific proteins (e.g., FOXA1 and caveolin1) reveals biphasic dynamics of the inhibition of the receptor-regulated transcriptional effects. Overall, we report a new model where to study the behavior of the Y537S ERα mutant that can be used for the identification of new targets and pathways regulating the Y537S ERα transcriptional activity.

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