Validation of A multiplex PCR detection kit for screening of herbicide-tolerant genes in genetically modified crops

2013 ◽  
Vol 56 (2) ◽  
pp. 251-254 ◽  
Author(s):  
Jae-Hwan Kim ◽  
Eun-Hee Kim ◽  
Mi-Chi Yea ◽  
Hae-Yeong Kim
2013 ◽  
Vol 7 (1) ◽  
pp. 21-29
Author(s):  
Ghaith Lotfi Aarif ◽  
Bilal Kamil Sulaiman ◽  
Zahra M. Alkhafaji

Detection of the genetically modified crops could be done by screening certain markers usually used in modification. In this study polymerase chain reaction (PCR) technology was used to investigate the presence of the promoter P35s and nos terminator in the genetically modified corn Zea mays. 72 samples of the maize crop collected from inside Iraqi market from various sources, including imported crops and other local strains used for agriculture or for the production of animal feed. DNA extracted from the corn seeds by two methods, the efficiency of extraction was compared between the two procedures, the purity of DNA samples extracted ranged between 1.4- 1.8 of the samples studied, while the ranged values for concentrations ranged from (500-2400) ng /µl, specificity of the DNA extracted was confirmed using Zea mays specific gene responsible for production of Zein protein, a storage protein. Results shows that all the samples were positive for this gene, results of the investigation of sequence responsible for regulating gene expression for promoter P35s and T-nos terminator, should that 10 samples 13.9% of the total 72 samples studied are genetically modified and gave positive results for the amplification of PCR using primers specialized for each of the P35s and T-nos. The results indicated that (9 out of 47) represent 19.14% of the samples studied imported for the government institutions were genetically modified. Multiplex PCR technique used for the detection of two types of the targets at the same reaction to reduce the time and efforts. Multiplex PCR successfully applied for two combinations of either zein and P35s or zein and nos.


10.5912/jcb77 ◽  
2004 ◽  
Vol 10 (3) ◽  
Author(s):  
Roger Turner

In 1998 the UK Government, in conjunction with Supply Chain Initiative on Modified Agricultural Crops (SCIMAC, a cross-industry group representing the supply chain), established a large-scale programme to examine the impact of genetically modified herbicide-tolerant crops on farmland biodiversity. The trials programme lasted three years and was conducted by a consortium of scientists who were responsible to an independent scientific subcommittee. The background to the establishment of these trials is described and the political pressures facing the Government at that time and the reactions of key stakeholders are discussed.The field-scale evaluations were a pioneering project to examine the impact of genetically modified crops on farmland biodiversity. The exercise was one of the largest experiments ever carried out to measure the environmental impact of specific farming practices, and shows that robust results can be obtained, while the resultant database represents a wealth of ecological information on three important arable crops – beet, maize and oilseed rape. It is clear that other such trials could be carried out to test other variables as part of a longer-term effort to steer farm management systems in ways more acceptable to society. However, in view of their costs (both to the UK Government and the technology providers) together with the adverse response by the environmental campaign groups, and the length of time needed to carry out the work, it seems unlikely that an exercise of similar scale will be undertaken in the near future.


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