Rnase A Enzyme Modification of Optimized SDS Protocol for DNA Extraction Suitable for Real-Time PCR Screening of GMOs

As the number of genetically modified crops increases rapidly, their accurate detection is significant for labelling and safety assessment. Currently, real-time PCR is the “golden standard” method for GMO detection. Hence, extraction of high quality DNA represents a crucial step for accurate and efficient DNA amplification. For GMO presence evaluation in the extracted DNA from raw corn kernels and roasted soybean, we used real-time PCR method, in consistent with the ISO17025 accreditation standards. As for DNA extraction, modified basic SDS protocol by adding RNase A enzyme in different steps of the protocol, with different time and temperature of incubation was used. The results showed as most suitable, the protocol where 10 µl of RNase A enzyme was added together with the lysis buffer at 65 °C for 30 minutes. Data for DNA yield and purity for roasted soybean was 469.6±3.3 µg/ml with A260/280 absorbance ratio 1.78±0.01. Suitability of DNA extracts for GMO analysis was assessed by screening for the presence of 35S promotor and Tnos terminator. Diluted extracts in concentrations 10, 1, 0.1, 0.01 and 0.0027 ng/µl, were tested in six replicates. Positive signal of amplification (LOD) was detected in all concentrations for both genetic elements in both matrices. The LOQ for 35S and Tnos for both matrices was 0.1 ng, while for Tnos in raw corn kernels was 0.01 ng. This in-house developed DNA extraction method is simple and obtains high-quality DNA suitable for GMO screening of 35S promotor and Tnos terminator in both raw and processed matrices.

MicroRNA Transformed Genetically Modified Crops and their Effect on Human Health.

GM crops or Genetically Modified crops are attracted a wide range of media attention in recent years and continues to do so. Media given awareness about the genetically modified crops to public. They reported the uses and drawbacks of the GM crops. The technique offers with regards to the range of advantages of the use of genetically modified crops. In the Pioneer stage of the production of GM crops, two different sectors of concern have been evolved, which includes impact on the agriculture and dangerous to human life. Safety of the eatables have a vital role in the world. The issue can be reduced by enhancing the productivity and quality of the crop. Genetic manipulation technology depends on the MiRNA, it is one of the main problem-solving methods, which influence the environmental product formation through improving major rules used for miRNAs modification and its objectives in GM plants, it contains constitutive, induction to stress, or specific tissue expression of micro RNAs or their aim, RNA gene silencing mechanism, micro-RNA-resistant target and gene expressions. Genetically Modified Organisms is one of the major focuses in biomedical research from 1980s. Since, Genetically Modified models with animal enable researchers for treatment of human genetic diseases. Genetically Modified microorganisms, crops, and animals are used for the production of drugs that are complex by which helps the generation to vaccines that are cheaper. However, this article+ more focused on the human health associated with the genetically modified foods and role of miRNAs in respected to GM food products.

A Label-Free Immunosensor Based on Gold Nanoparticles/Thionine for Sensitive Detection of PAT Protein in Genetically Modified Crops

Genetically modified (GM) crops containing phosphinothricin acetyltransferase (PAT) protein has been widely planted worldwide. The development of a rapid method for detecting PAT protein is of great importance to food supervision. In this study, a simple label-free electrochemical immunosensor for the ultrasensitive detection of PAT protein was constructed using thionine (Thi)/gold nanoparticles (AuNPs) as signal amplification molecules and electrochemically active substances. Under optimum conditions, the limits of detection of the sensor for soybean A2704-12 and maize BT-176 were 0.02% and 0.03%, respectively. The sensor could detect crops containing PAT protein and had no cross-reaction with other proteins. After storage at 4°C for 33 days, the sensor still retained 82.5% of the original signal, with a relative standard deviation (RSD) of 0.92%. The recoveries of the sensor for soybean A2704-12 and maize BT-176 were 85%–108% and 98%–113%, respectively. The developed PAT-target immunosensor with high sensitivity, specificity, and satisfactory reproducibility and accuracy will be a useful tool in the trace screening of GM crops. Moreover, this design concept can be extended to other proteins by simply changing the antibody.

What to feed or what not to feed-that is still the question

Introduction This review addresses metabolic diversities after grain feeding of cattle using artificial total mixed ration (TMR), in place of pasture-based feeding. Objectives To determine how grain feeding impairs the deuterium-depleting functions of the anaplerotic mitochondrial matrix during milk and meat production. Methods Based on published data we herein evaluate how grain-fed animals essentially follow a branched-chain amino acid and odd-chain fatty acid-based reductive carboxylation-dependent feedstock, which is also one of the mitochondrial deuterium-accumulating dysfunctions in human cancer. Results It is now evident that food-based intracellular deuterium exchange reactions, especially that of glycogenic substrate oxidation, are significant sources of deuterium-enriched (2H; D) metabolic water with a significant impact on animal and human health. The burning of high deuterium nutritional dairy products into metabolic water upon oxidation in the human body may contribute to similar metabolic conditions and diseases as described in state-of-the-art articles for cows. Grain feeding also limits oxygen delivery to mitochondria for efficient deuterium-depleted metabolic water production by glyphosate herbicide exposure used in genetically modified crops of TMR constituents. Conclusion Developments in medical metabolomics, biochemistry and deutenomics, which is the science of biological deuterium fractionation and discrimination warrant urgent critical reviews in order to control the epidemiological scale of population diseases such as diabetes, obesity and cancer by a thorough understanding of how the compromised metabolic health of grain-fed dairy cows impacts human consumers.

Indicators of Complexity and Over-Complexification in Global Food Systems

Global food systems have increased in complexity significantly since the mid-twentieth century, through such innovations as mechanization, irrigation, genetic modification, and the globalization of supply chains. While complexification can be an effective problem-solving strategy, over-complexification can cause environmental degradation and lead systems to become increasingly dependent on external subsidies and vulnerable to collapse. Here, we explore a wide array of evidence of complexification and over-complexification in contemporary global food systems, drawing on data from the Food and Agriculture Organization and elsewhere. We find that food systems in developed, emerging, and least developed countries have all followed a trajectory of complexification, but that return on investments for energy and other food system inputs have significantly declined—a key indicator of over-complexification. Food systems in developed countries are further along in the process of over-complexification than least developed and emerging countries. Recent agricultural developments, specifically the introduction of genetically modified crops, have not altered this trend or improved return on investments for inputs into food systems. Similarly, emerging innovations belonging to the “digital agricultural revolution” are likewise accompanied by energy demands that may further exacerbate over-complexification. To reverse over-complexification, we discuss strategies including innovation by subtraction, agroecology, and disruptive technology.

An Outlook on Global Regulatory Landscape for Genome-Edited Crops

The revolutionary technology of CRISPR/Cas systems and their extraordinary potential to address fundamental questions in every field of biological sciences has led to their developers being awarded the 2020 Nobel Prize for Chemistry. In agriculture, CRISPR/Cas systems have accelerated the development of new crop varieties with improved traits—without the need for transgenes. However, the future of this technology depends on a clear and truly global regulatory framework being developed for these crops. Some CRISPR-edited crops are already on the market, and yet countries and regions are still divided over their legal status. CRISPR editing does not require transgenes, making CRISPR crops more socially acceptable than genetically modified crops, but there is vigorous debate over how to regulate these crops and what precautionary measures are required before they appear on the market. This article reviews intended outcomes and risks arising from the site-directed nuclease CRISPR systems used to improve agricultural crop plant genomes. It examines how various CRISPR system components, and potential concerns associated with CRISPR/Cas, may trigger regulatory oversight of CRISPR-edited crops. The article highlights differences and similarities between GMOs and CRISPR-edited crops, and discusses social and ethical concerns. It outlines the regulatory framework for GMO crops, which many countries also apply to CRISPR-edited crops, and the global regulatory landscape for CRISPR-edited crops. The article concludes with future prospects for CRISPR-edited crops and their products.

Analysis of gm crops allowed for using in the european union

The development of biotechnology in the field of GMOs requires states to take specific decisions to regulate the spread of genetically modified crops. In the European Union all GM crops that circulation are subject to mandatory registration, which regulates the placing on the market and circulation of genetically modified raw materials, food and feed. The article presents systematized data about the registration of genetically modified soybean, maize and rapeseed in the European Union. It was established that most of the GM crops have introduced genes that give them tolerance to herbicides of different groups. The register of the European Union currently includes 12 events of soybean (GTS 40-3-2, A2704-12, Mon 89788, MON87705, DP 356043, A5547-127, FG 72, SYHTOH 2, DAS-44406-6, DAS-68416- 4, Mon 87708, BPS-CV127-9), 5 events of maize (MZHG0JG, DAS-40278-9, GA 21, NK 603, T 25), 3 events of rapeseed (GT 73, T45, TOPAS 19/2) with tolerance to herbicides. It has been shown that a significant number of registered GM plants have a combination of several events, including tolerance to herbicides and resistance to certain insects or improving quality features of crops. Among them are one event of soybean (DP305423-1), 9 events of maize (TC 1507, DP 4114-3, MON 87411-9, MON 87427, MON 88017, DAS59122-7, Bt 176, Bt 11, DAS 1507) and one event of rapeseed (MS8xRF3). Many GM crops (one event of soybean and 6 events of maize) have introduced genes that determine the plant's tolerance to insects. Only a tiny amount of GM crops are being with altered consumer or technological qualities. In the register of genetically modified crops, all events of GM crops are currently authorized for usage for food, supplements, feed and other product. А single event of maize (Mon 810), that was allowed for cultivation at the time of this analysis was at the stage of renewal of the permit.

Correlating Genetically Modified Crops, Glyphosate Use and Increased Carbon Sequestration

In the early 1990s, tillage was the leading form of weed control, with minimum/zero-tillage management practices incapable of long-term continuation. Presently, weed control through tillage has virtually disappeared as cropland management systems have transitioned largely to continuous cropping, with zero to minimal soil disturbance. Research was undertaken to examine what was driving this land management transition. A carbon accounting framework incorporating coefficients derived from the Century Model was used to estimate carbon sequestration in the Canadian province of Saskatchewan. The results quantify the transition from farmland being a net carbon emitter to being a net carbon sequesterer over the past 30 years. This evidence confirms the correlation between genetically modified, herbicide-tolerant crops and glyphosate use is a driver of the increased soil carbon sequestration. The removal of tillage and adoption of minimal soil disturbances has reduced the amount of carbon released from tillage and increased the sequestration of carbon through continuous crop production. Countries that ban genetically modified crops and are enacting legislation restricting glyphosate use are implementing policies that Canadian farm evidence indicates will not contribute to increasing agricultural sustainability.