Effect of In Vitro Copper and Zinc Supplementation on Neutrophil Phagocytic Activity and Lymphocyte Proliferation Response of Transition Dairy Cows

2015 ◽  
Author(s):  
Kalyan De ◽  
Shashi Pal ◽  
Joydip Mukherjee ◽  
Shiv Prasad ◽  
Ajay Kumar Dang
Keyword(s):  
Dairy Cows ◽  
Phagocytic Activity ◽  
Lymphocyte Proliferation ◽  
Zinc Supplementation ◽  
Copper And Zinc ◽  
Lymphocyte Proliferation Response ◽  
Transition Dairy Cows ◽  
Proliferation Response

scholarly journals In VitroImmune Competence of Buffaloes (Bubalus bubalis) of Different Production Potential: Effect of Heat Stress and Cortisol

2011 ◽  
Vol 2011 ◽  
pp. 1-5 ◽  
Author(s):  
Joydip Mukherjee ◽  
Sujata Pandita ◽  
Ruokuobeinuo Huozha ◽  
Manju Ashutosh
Keyword(s):  
Heat Stress ◽  
Stimulation Index ◽  
Bubalus Bubalis ◽  
Potential Effect ◽  
Production Level ◽  
Cell Mediated Immunity ◽  
Lymphocyte Proliferation Response ◽  
Proliferation Response

Twelve healthy lactating Murrah buffaloes of similar parity (3rd) between 90 and 120 days of lactation, selected from the herd of National Dairy Research Institute (Karnal, India) and maintained at managemental practices as followed at the Institute they were included in this experiment. The animals were divided into two groups based on their production level in previous lactation. The average milk production level of group 1 and II was 9.3 and 6 lit/day, respectively. Blood was collected from these buffaloes on three occasions 10 days apart. The lymphocytes were separated and cultured in RPMI 1640 medium with PHA-P for 24 h at 37°C in a humidified CO2incubator (95% air and 5%  CO2). The lymphocyte responsiveness was also evaluated in response to thein vivoheat stress andin vitrocortisol. Mitogen-induced stimulation index was not affected by production level (). Stimulation index was significantly reduced () in both the groups when cortisol was added at 2.0 ng level in the culture. However, in heat-stressed buffaloes stimulation index did not vary despite increasing levels of cortisol, thus indicating that lymphocyte may become cortisol resistant during periods of acute heat stress. The results showed that lymphocyte proliferation response can be effectively used to study buffalo cell-mediated immunityin vitro.

Immunoenhancing Effects of a New Probiotic Strain, Lactobacillus fermentum PL9005

2005 ◽  
Vol 68 (3) ◽  
pp. 571-576 ◽  
Author(s):  
JONG-HWAN PARK ◽  
YEONHEE LEE ◽  
ENPYO MOON ◽  
SEUNG-HYEOK SEOK ◽  
SUN-A CHO ◽  
...  
Keyword(s):  
Small Intestine ◽  
Lymphocyte Proliferation ◽  
Immunoglobulin A ◽  
Clinical Signs ◽  
Probiotic Strain ◽  
Lactobacillus Fermentum ◽  
Control Group ◽  
Lactic Acid Concentration ◽  
Lymphocyte Proliferation Response ◽  
Proliferation Response

The immunoenhancing effects of Lactobacillus fermentum PL9005 were assessed via mouse intragastric inoculation. The number of immunoglobulin A–positive cells in the small intestine, CD4+ T lymphocytes in the peripheral blood, and the lymphocyte proliferation response to mitogen stimulation (lipopolysaccharide) increased in mice fed L. fermentum PL9005. The lactic acid concentration also increased dose dependently in the small intestine of mice fed L. fermentum PL9005. No differences were found in body weight, food intake, and clinical signs between mice fed L. fermentum PL9005 and the control group. Results indicated that L. fermentum PL9005 is a probiotic with immunoenhancing properties.

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