In vitro immunomodulatory activity test of Bengle rhizoma extract (Zingiber cassumunar Roxb.): phagocytic activity of macrophages and lymphocyte proliferation in mice

In the present study, an attempt was made to screen immunomodulatory activity of the hydroalcoholic extract (HEDF) of Dendrophthoe falcata (L.f.) Ettingsh (Loranthaceae), an Indian Ayurvedic plant, on different arms of the immune system. HEDF was evaluated for immunological function by studying delayed type hypersensitivity (DTH) to sheep RBCs, nitric oxide (NO) release from murine peritoneal macrophages, phagocytic activity of polymorphonuclear (PMN) cells in vitro and reticuloendothelial system in vivo, plaque forming cell response of splenic lymphocytes to sheep erythrocytes, haemagglutination antibody titer and neutrophil adhesion test. Significant increase in NO production by mouse peritoneal macrophages was detected in culture supernatants indicated increased phagocytic activity of macrophages. After post oral administration of HEDF in three doses of 250, 475 and 950 mg/kg body weight, a significant increase in phagocytic activity of PMN cells/reticuloendothelial system, stimulation of neutrophil function and splenic antibody secreting cells, were also noticed. Stimulation of humoral immune response was further observed with elevation in haemagglutination antibody titer. Heightened DTH reaction suggested convincing evidence for activation of cellular immune system. Present study thus confirms the immunomodulatory activity of the hydroalcoholic extract of D. falcata and the immunomodulatory responses were found to be dose dependent manner.Keywords: Dendrophthoe falcata; Antibody titer; Neutrophil adhesion; Phagocytic activity.© 2011 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved.doi:10.3329/jsr.v3i3.7655 J. Sci. Res. 3 (3), 629-640 (2011)

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In Vitro Immunomodulatory Activity of Aqueous Quercus infectoria Gall Extract

Oman Medical Journal ◽

10.5001/omj.2021.63 ◽

2021 ◽

Vol 36 (3) ◽

pp. e265-e265

Author(s):

Wan Abdul Wahab Wan-Nor-Amilah ◽

Mohd Latif Syifaa’-Liyana ◽

Yahya Azlina ◽

Zainuddin Shafizol ◽

Asma Abdullah Nurul

Keyword(s):

Phagocytic Activity ◽

Enzyme Linked Immunosorbent Assay ◽

Immunomodulatory Activity ◽

No Production ◽

Griess Reaction ◽

Cytokine Levels ◽

Proliferative Assay ◽

And Control ◽

Quercus Infectoria

Objectives: Our study reports the immunomodulatory potency of Quercus infectoria gall extract in vitro. The aqueous extract was prepared and examined for its effects on cell proliferation, phagocytic activity, nitric oxide (NO) production, and cytokine synthesis by murine macrophages. Methods: Proliferative, phagocytic activity, and NO production of extract-treated and control cells were studied using proliferative assay, flow cytometry, and Griess reaction, respectively. An enzyme-linked immunosorbent assay was performed to determine the levels of pro- and anti-inflammatory cytokines in the macrophage culture. Results: Treated macrophages had a higher proliferative rate and phagocytic activity compared to untreated macrophages. The cell treatment with an extract concentration of 64 μg/mL demonstrated a significant decrease in NO production (p < 0.001). An increase in cytokine levels (IL-2, IL-5, IL-10, IL-17A, IL-23, TGF-β1) was observed; however, this increase was not statistically significant. Conclusions: Our study suggests that gall extract possesses the potential for augmenting immunomodulatory activity by cellular mediated mechanism and could play a role in regulating the innate immune response.

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Effect of In Vitro Copper and Zinc Supplementation on Neutrophil Phagocytic Activity and Lymphocyte Proliferation Response of Transition Dairy Cows

Agricultural Research ◽

10.1007/s40003-015-0181-7 ◽

2015 ◽

Cited By ~ 1

Author(s):

Kalyan De ◽

Shashi Pal ◽

Joydip Mukherjee ◽

Shiv Prasad ◽

Ajay Kumar Dang

Keyword(s):

Dairy Cows ◽

Phagocytic Activity ◽

Lymphocyte Proliferation ◽

Zinc Supplementation ◽

Copper And Zinc ◽

Lymphocyte Proliferation Response ◽

Transition Dairy Cows ◽

Proliferation Response

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Immunomodulatory and Antioxidant Activity of Green Grass Jelly Leaf Extract (Cyclea barbata Miers.) In Vitro

Advances in Tropical Biodiversity and Environmental Sciences ◽

10.24843/atbes.2018.v02.i01.p03 ◽

2018 ◽

Vol 2 (1) ◽

pp. 10 ◽

Cited By ~ 1

Author(s):

Mustafid Rasyiid ◽

Rendi Mahadi ◽

Krisnanda Surya Dharma ◽

Lindia Anggraini ◽

Rahma Nurdiyanti ◽

...

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Phagocytic Activity ◽

Ethyl Acetate Extract ◽

Activity Index ◽

Ethanol Extract ◽

Chloroform Extract ◽

Immunomodulatory Activity ◽

Leaf Extracts

Green grass jelly (Cyclea barbata Miers.) is known for its benefit to human health especially in supporting body’s immune system and wellness. This research aimed to determine immunomodulatory and antioxidant activity of green grass jelly leaf extracts in vitro. Old leaves were collected as sample then dried and ground to powder. The extraction was done with sohxletation using three different solvents, chloroform, ethyl acetate, and ethanol. The immunomodulatory activity was evaluated by treating the crude extracts at concentrations of 50, 100, and 500 mg/mL on macrophages of rat in vitro. The treated macrophage was then challenged for their phagocytic activity to latex beads. The antioxidant activity was done using 1,1-diphenil-2-picrilhydrazil (DPPH) with spectrophotometry technique. All treatments were done with three replicates. Detection of the bioactive groups of the extracts was done by Thin Layer Chromatography (TLC). The results showed that ethyl acetate extract has the highest phagocytic activity followed with chloroform extract and ethanol extract, respectively. Optimum concentration was reached at 100 mg/mL of ethyl acetat extract. The ethyl acetate extract was also the extract with the highest antioxidant activity index 7.7 followed by both extracts of chloroform and ethanol with similar index value of 6.25 and 6.3, respectively. The ethyl acetate extract contained phenolics, flavonoids, tannins, and terpenoids.

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Extraction, Characterization and Immunological Activity of Polysaccharides from Cynomorium songaricum Rupr.

Journal of Biomaterials and Tissue Engineering ◽

10.1166/jbt.2021.2736 ◽

2021 ◽

Vol 11 (8) ◽

pp. 1543-1554

Author(s):

Min Li ◽

Jianhua Yang ◽

Junping Hu

Keyword(s):

Inflammatory Cytokines ◽

Phagocytic Activity ◽

Active Component ◽

Immunomodulatory Activity ◽

Immunological Activity ◽

Raw264.7 Macrophages ◽

Immunosuppressed Mice

Cynomorium songaricum Rupr. (CSP) have been used widely in TCM for many years, polysaccharides from CSP are main active component. In our previous research, we found that CSP play a role of immunomodulatory activity in vitro, but its mechanisms and in vivo immunomodulatory activity hadn’t been explored. In present study, we firstly extracted CSP and identified two new fractions CSP-a, CSP-b. To assess the immunomodulatory activity of CSP in vivo, cyclophosphamide (CTX)-induced immunosuppressed mice models were generated and then treated with CSP. The results demonstrated that CSP could improve thymus and spleen indices, phagocytic and clearance index, serum hemolysin, inflammatory cytokines productions in serum. To explore the mechanisms of CSP, CSP-a, CSP-b, RAW264.7 macrophages were used to evaluate the immunomodulatory activity in vitro, the results demonstrated that CSP, CSP-a, CSP-b can induce macrophage proliferation, enhance the phagocytic activity and increase cytokines expression. CSP, CSP-a, CSP-b possessed the immunomodulatory activity by inducing the phosphorylation of MAPKs. This study suggested that CSP may be useful for lessening chemotherapy-induced immunosuppression and proposed the basis for the clinical application of CSP.

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ISOLASI SENYAWA AKTIF DAN UJI AKTIVITAS EKSTRAK JAHE MERAH (ZINGIBER OFFICINALE) SEBAGAI IMUNOMODULATOR

Jurnal Riset Kefarmasian Indonesia ◽

10.33759/jrki.v3i2.131 ◽

2021 ◽

Vol 3 (2) ◽

pp. 77-91

Author(s):

Masniah Masniah ◽

Jafril Rezi ◽

Ahmad Purnawarman Faisal

Keyword(s):

Lymphocyte Proliferation ◽

Ethanol Extract ◽

Zingiber Officinale ◽

Immunomodulatory Activity ◽

Ginger Extract ◽

Immunostimulatory Activity ◽

Macrophage Cells ◽

Macrophage Phagocytosis ◽

Red Ginger

Red ginger (Zingiber officinale) contains many chemical compounds that have many activities. This study aims to determine the active compounds of Zingiber officinale and immunomodulatory activity that can increase the phagocytosis of macrophage cells and lymphocyte cell proliferation. Red ginger extract compounds were identified using GC-MS. The parameters of immunostimulatory activity were macrophage phagocytosis and lymphocyte proliferation, the preparations were made in several extract concentrations. This research is an experimental study in the laboratory. The research included fresh red ginger, made into ethanol extract, dried. The results were made a suspension, the extract was then given to macrophage cells (phagocytosis testing) and lymphocyte cells (proliferation testing) in vitro to test the immunomodulatory effect. The results showed that the most active extract in macrophage phagocytosis activity was the extract concentration of 500 ppm. While the activity of lymphocyte proliferation was shown by the concentration of group A (Cell, 500 ppm extract, MK, Engerik). The active compound contained in the ethanol extract of red ginger is thought to be Geraniol

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Bioactivity Assay of Chitosan Microspheres Containing Pig Thymosin In Vitro

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.830.455 ◽

2013 ◽

Vol 830 ◽

pp. 455-458

Author(s):

Pei Ni ◽

Rong Rong Zhan ◽

Yu Long Wei ◽

Jing Hui ◽

Feng Qing Hu

Keyword(s):

Lymphocyte Proliferation ◽

Mouse Spleen ◽

Cross Linking ◽

Immunomodulatory Activity ◽

Spleen Lymphocyte ◽

Chitosan Microspheres ◽

Binding Rate ◽

Bioactivity Assay ◽

Scope Of Application

Thymosin, composed of many peptides, is distributed in animal and has immunomodulating effects. However, many factors could affect its bioactivity. Chitosan microspheres containing pig thymosin (CMPT) were prepared through W/O emulsion cross-linking method. In this study, thymosin activity between pig-thymosin and CMPT was investigated through E-rosette test and mouse spleen lymphocyte proliferation (MPLP) experiment in vitro. Results showed that CMPT has the same immunomodulatory activity as pig thymosin. When pig-thymosin was 1.5 μg / ml, E-rosette binding rate was 63%, and E-rosette binding rate of CMPT reached 60%。MPLP rate was 73.3% and 68.5%, respectively. CMPT have the capacity to keep the E-rosette binding and MPLP activity of pig thymosin. This may help to improve traditional formulation and bioavailability of thymosin, and expand its scope of application in clinic.

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Immunomodulatory Activity Test of Syrup Dosage Form of Combination Phyllantus niruri Linn. And Sterculia quadrifida R.Br. Extract

International Journal of Research in Pharmaceutical Sciences ◽

10.26452/ijrps.v11i1.1806 ◽

2020 ◽

Vol 11 (1) ◽

pp. 191-199 ◽

Cited By ~ 1

Author(s):

Rollando Rollando ◽

Mauren Engracia ◽

Eva Monica ◽

Siswadi Siswadi

Keyword(s):

Bark Extract ◽

Immunomodulatory Activity ◽

Macrophage Activity ◽

Lattice Design ◽

Activity Test ◽

Tnf Α ◽

Simplex Lattice

Meniran (Phyllantus niruri Linn.) is used generally as an immunomodulator and hepatoprotector. Empirically, bark of faloak (Sterculiaqudrifida R.Br.) is used as a medicinal plant to cure diseases such as hepatitis, gastroentritis, and diabetes. The combination of meniran extract and bark of faloak extract has the potential as a natural immunomodulatory agents. Immunomodulators are compositions of either recombinant, synthetic, or natural, which can restore the immune system. Therefore it is necessary to optimize the optimal formula in obtaining syrup preparations which have optimal immunomodulatory activity. The aim of this study was to find out the optimal syrup combination of meniran extract and faloak bark extract to produce optimal response values in the in vitro immunomodulatory activity test with TNF-α and NF-κB parameters, and immunomodulatory activity tests in vivo with activity parameters macrophages. The extract was obtained through reflux extraction. The results of the extract were formulated and approved to determine their ability to induce TNF-α and NF-κB, and in the test of mice balb/b (Mus musculus L.) orally for 21 days. The lymph organs were isolated, rinsed with an RPMI-1640 solution, and analyzed using ELISA reader 450 nm. The determination of optimal formulas is done by using Rstudio v.3.5.3 software with ANOVA test parametric test and using Design Expert v.11 software using the Simplex Lattice Design (SLD) method. The results showed that the formula for the preparation of combination syrup to produce results induced TNF-α and NF-κB, and macrophage activity, namely the formula using meniran and faloak 0.96993: 0.0300704.

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