In vitro somatic embryogenesis and plantlet regeneration in anchote [Coccinia abyssinica (Lam.) Cong.]

Nodal segments of sandalwood were cultured on MS medium amended with different plant growth regulators in varying concentrations to search out higher in vitro response leading to plantlet regeneration via somatic embryogenesis and/or organogenesis. Higher proportion of direct somatic embryogenesis, number(s) of somatic embryo per explant and plantlet regeneration via direct organogenesis were recorded on MS medium supplemented with a moderate concentration of TDZ (1.0 mgl-1) in combination with comparatively a lower concentration of NAA (0.5 mgl-1). A relative higher concentration of BAP (1.0-2.0 mgl-1) in combination with a lower concentration of NAA (0.5 mgl-1) promoted frequency of indirect somatic embryogenesis. Ratio of organ formation directly from surface of cultured explants was recovered from culture medium fortified with a higher concentration of BA at the concentration of 4.0 mgl-1 in combination with a lower concentration of NAA (0.5 mgl-1). Maximum plantlets regenerated via somatic embryogenesis (direct and/or indirect) on regeneration medium supplemented with 2.0 mgl-1TDZ in combination with 1.0 mg l-1GA3, while plantlets in higher frequencies via indirect organogenesis was attained with regeneration medium amended with comparatively lower concentration of TDZ (1.0 mg l-1) in combination with 0.5 mgl-1 GA3 and 0.5 mgl-1 NAA. The plantlets were transferred to pots and hardened in Environmental Growth Cabinet and Net House during preliminary weaning period and transferred to field successfully. Morphologically normal plants were recovered.

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Somatic embryogenesis and in vitro plantlet regeneration in Salicornia brachiata Roxb.

Plant Cell Tissue and Organ Culture (PCTOC) ◽

10.1007/s11240-014-0571-8 ◽

2014 ◽

Vol 120 (1) ◽

pp. 355-360 ◽

Cited By ~ 13

Author(s):

Mangal S. Rathore ◽

Nikita Paliwal ◽

K. G. Vijay Anand ◽

Pradeep K. Agarwal

Keyword(s):

Somatic Embryogenesis ◽

Plantlet Regeneration ◽

Salicornia Brachiata

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EMBRIOGENESIS SOMATIK IN VITRO DAN REGENERASI PLANLET DARI TIGA VARIETAS ALFALFA (Medicago sativa L.)

Jurnal Bioteknologi & Biosains Indonesia (JBBI) ◽

10.29122/jbbi.v6i1.3348 ◽

2019 ◽

Vol 6 (1) ◽

pp. 83

Author(s):

Sulastri Sulastri ◽

Winda Nawfetrias ◽

Djatmiko Pinardi ◽

Henti Rosdayanti

Keyword(s):

Somatic Embryogenesis ◽

Medicago Sativa ◽

Callus Induction ◽

Plantlet Regeneration ◽

Dichlorophenoxyacetic Acid ◽

Naphthaleneacetic Acid ◽

Medicago Sativa L ◽

Embryogenic Callus Induction ◽

2,4 Dichlorophenoxyacetic Acid

In Vitro Somatic Embryogenesis and Plantlet Regeneration of Three Varieties of Alfalfa (Medicago sativa L.)ABSTRACTAlfalfa (Medicago sativa L.) is a valuable plant as a source of food for animal, forage, pharmaceutical, medicine, food supplement, and human consumption. In vitro selection technology combined with induction or spontaneous mutagenesis has been effective in altering or isolating genetic variability for desirable characters. Consequently, a reproducible in vitro propagation technique of that plant is mandatory. The aim of the research was to obtain information on the embryogenic callus induction, somatic embryogenesis, and plantlet regeneration of three varieties of alfalfa. The results showed that an optimum embryogenic callus induction (82%) was obtained on Murashige & Skoog (MS) basal medium containing 2 ppm 2,4-dichlorophenoxyacetic acid (2,4-D), 2 ppm kinetin and 2 ppm a-naphthaleneacetic acid (NAA). Those embryogenic calli could subsequently develop into somatic embryos, which germinated and regenerated into normal plantlets on R1 medium consisting of MS nutrients without the addition of plant growth regulator.Keywords: alfalfa, callus, embryogenic, plantlets, regeneration ABSTRAKAlfalfa (Medicago sativa) adalah tanaman berharga sebagai sumber makanan untuk hewan, yaitu hijauan pakan ternak, farmasi, obat-obatan, suplemen makanan dan konsumsi manusia. Teknologi seleksi in vitro yang dikombinasikan dengan induksi atau mutagenesis spontan telah terbukti efektif dalam mengubah atau mengisolasi variabilitas genetik untuk karakter yang diinginkan. Oleh sebab itu, keberhasilan teknik perbanyakan in vitro yang telah terbukti dapat direproduksi dari tanaman tersebut menjadi syarat yang harus terpenuhi. Tujuan dari penelitian ini adalah untuk mendapatkan informasi mengenai induksi kalus embriogenik, embriogenesis somatik dan regenerasi planlet dari tiga varietas alfalfa. Hasil penelitian menunjukkan bahwa induksi kalus embriogenik optimal (82%) didapat pada media Murashige & Skoog (MS) dengan 2 ppm 2,4-dichlorophenoxyacetic acid (2,4-D), 2 ppm kinetin dan 2 ppm a-naphthaleneacetic acid (NAA). Kalus embriogenik tersebut dapat membentuk embrio somatik, embrionya berkecambah dan beregenerasi membentuk planlet normal pada perlakuan media R1 yaitu nutrisi MS tanpa penambahan zat pengatur tumbuh.Kata Kunci: alfalfa, embriogenik, kalus, planlet, regenerasi

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REGENERASI TANAMAN OBAT KELADI TIKUS (Thyponium flagelliform L. Blume) MELALUI EMBRIOGENESIS SOMATIK SECARA IN VITRO

Jurnal Sains dan Teknologi Indonesia ◽

10.29122/jsti.v14i1.904 ◽

2013 ◽

Vol 14 (1) ◽

Author(s):

Juwartina Ida Royani ◽

Indah Sulistyorini ◽

Dwi Rizkyanto Utomo

Keyword(s):

Somatic Embryogenesis ◽

Cell Division ◽

Plant Growth ◽

Growth Regulator ◽

Plant Growth Regulator ◽

Somatic Embryos ◽

Somatic Cells ◽

Plantlet Regeneration ◽

Embryonic Cells

Somatic embryogenesis is a process whereby somatic cells have differentiation in somatic embryos and it’s formed from embryonic cells due to the high polarization of uncontrolled cell division. The aim of this research is to produced shoots of rodent tuber via somatic embryos.Induction of somatic embryos from explants in MS media with N (2-chloro-4-pyridyl)-Nphenyl urea (CPPU) and continuous subculture in MS media without plant growth regulator for 1 month. Plantlet regeneration growth in liquid MS media with BAP and IBA fortified with 40 gr/l of sucrose in the dark. The result showed that somatic embryonic appears in media induction after 2 week and plantlet regeneration in 4 week. All plantlet growed from embryosomatic can survive 100% in field with normal shoot.

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