Impact of unilateral eyestalk ablation on Callinectes arcuatus (Ordway, 1863) under laboratory conditions: behavioral evaluation

Eyestalk ablation allows the removal of neurohormones that inhibit early reproduction and decreases the time to complete the molting cycle. The present study evaluated the impact of unilateral eyestalk ablation on the behavior of blue crab breeders Callinectes arcuatus in the short term under laboratory conditions. Immature male and female crabs and individuals in the intermolt stage were collected in Ohuira Bay, Ahome, Sinaloa, Mexico. Three bioassays were performed: males, females, and male-females each with three treatments; control + xylocaine (50 μL, 5%), squash ablation + xylocaine (50 μL, 5%), ablation by string ligation + xylocaine (50 μL, 5%), each with three replicas and three periods of evaluation after the ablation procedure (10 min, 2 h, and 24 h). Kruskal-Wallis and Chi-square (χ2) tests were used to evaluate significances (P < 0.05) in the behavior of C. arcuatus. The sizes of blue crab C. arcuatus were 9.72 ± 1.0 cm of carapace width. The feeding behavior of the male-females bioassay was significant at 2 h (P < 0.05). The results obtained were satisfactory using two techniques, unilateral eyestalk ablation by squash and ligation in which pain and aggressiveness indicators were mitigated. Disorientation and aggressiveness in males were significant at 2 h (P < 0.05), and in females after 10 min, and 2 h after ablation (P < 0.05). In the male-females bioassay, aggressiveness was significant at 2 h (P < 0.05). Courtship and copulation were recorded in those crabs subjected to unilateral eyestalk squash ablation (13 and 8, respectively).

Strong regulatory effects of vgll3 genotype on reproductive axis gene expression in immature male Atlantic salmon

Age at maturity is a major contributor to the diversity of life history strategies in organisms. The process of maturation is influenced by both genetics and the environment, and includes changes in levels of sex hormones and behavior, but the specific factors leading to variation in maturation timing are not well understood. gnrh1 regulates the transcription of gonadotropin genes at the onset of puberty in many species, but this gene is lacking in certain teleost species including Atlantic salmon (Salmo salar), which raises the possibility of the involvement of other important regulatory factors during this process. Earlier research has reported a strong association of alternative alleles of the vgll3 gene with maturation timing in Atlantic salmon, suggesting it as a potential candidate regulating reproductive axis genes. Here, we investigated the expression of reproductive axis genes in immature Atlantic salmon males with different vgll3 genotypes during the spawning period. We detected strong vgll3 genotype-dependent differential expression of reproductive axis genes (such as fshb, lhb, amh and igf3) tested in the pituitary, and testis of one-year-old immature male Atlantic salmon. In addition, we observed differential expression of jun (ap1) and nr5a1b (sf1), potential upstream regulators of gonadotropins in the pituitary, as well as axin2, id3, insl3, itch, ptgs2a and ptger4b, the downstream targets of amh and igf3 in the testis. Hereby, we provide evidence of strong vgll3 genotype-dependent transcriptional regulation of reproductive axis genes prior to sexual maturation together with models for distinct actions of vgll3 genotypes on the molecular processes controlling spermatogenesis in Atlantic salmon.

Physeal injury in a skeletally immature male athlete

A 12-year-old skeletally immature male athlete presented for evaluation with acute bilateral knee pain. Initial radiographs revealed subtle lucency of the medial proximal tibial physis and MRI was recommended. On MRI, broad-based intravasation of the physis into the proximal femoral and tibial physes was observed. This represented physeal widening, a phenomenon that has been observed in skeletally immature athletes presenting for acute knee pain. While such changes to the physis may be quite prominent on MRI, conventional radiographic findings may be much more subtle. As many causes of chronic knee pain in this population are managed with active rehabilitation, a high index of suspicion for this diagnosis in the presence of physeal abnormalities may warrant evaluation with MRI. The literature suggests this pathology will resolve with rest, but, if weight-bearing activities are continued, there is a risk for significant growth abnormalities.

Never Grow Up: The Queerness Of Immaturity In American Film

This dissertation examines the contradictory status of immaturity in our culture. While immaturity’s otherness to heteronormative adulthood is a source of fear and anxiety, this otherness is also what makes it desirable–that is, immaturity represents an escape from the pressures and standards of adulthood, signifying an oppositional subjectivity reminiscent of youthful rebellion. Indeed, the oppositional nature of immaturity is ultimately what gives it power as a source of political agency. Rather than seeing immaturity as something to be ashamed of, or as something to be avoided or defeated, this dissertation, following Judith/Jack Halberstam, views immaturity as a powerful form of resistance as well as a queer “way of being.” In fact, these two latter elements–resistance and queerness–go hand-in-hand; queerness within this project is primarily understood as an uncompromising “resistance to regimes of the normal,” specifically those pertaining to maturity and success. Beginning with a focus on male immaturity, I establish the fear/desire dynamic characterizing the immature male through close readings of the 1950s male-centered melodrama, combined with an historically-oriented analysis of postwar American culture. Next, I examine how comedy and dramedy films about childish men from the 1980s to the present day are also structured by this contradictory dynamic, as they both resist and reinforce heteronormative adulthood. My textual analysis of these films are grounded in theories of queer temporality. Finally, I focus on the female counterpart to the immature male, examining various constructions of female immaturity in recent cinema. Here, I utilize a broader range of queer temporal theories to demonstrate the political potential of immature womanhood, where “immature” gains agency through its queer and feminist resistance to the tyranny of heteronormative adulthood. While this dissertation ultimately seeks to demonstrate how immaturity functions as a site of resistance to (hetero)normativity, it also acknowledges how it can (specifically in the context of male homosociality), reinforce and reproduce oppressive structures, further underlining immaturity’s incongruous status.

Never Grow Up: The Queerness Of Immaturity In American Film

This dissertation examines the contradictory status of immaturity in our culture. While immaturity’s otherness to heteronormative adulthood is a source of fear and anxiety, this otherness is also what makes it desirable–that is, immaturity represents an escape from the pressures and standards of adulthood, signifying an oppositional subjectivity reminiscent of youthful rebellion. Indeed, the oppositional nature of immaturity is ultimately what gives it power as a source of political agency. Rather than seeing immaturity as something to be ashamed of, or as something to be avoided or defeated, this dissertation, following Judith/Jack Halberstam, views immaturity as a powerful form of resistance as well as a queer “way of being.” In fact, these two latter elements–resistance and queerness–go hand-in-hand; queerness within this project is primarily understood as an uncompromising “resistance to regimes of the normal,” specifically those pertaining to maturity and success. Beginning with a focus on male immaturity, I establish the fear/desire dynamic characterizing the immature male through close readings of the 1950s male-centered melodrama, combined with an historically-oriented analysis of postwar American culture. Next, I examine how comedy and dramedy films about childish men from the 1980s to the present day are also structured by this contradictory dynamic, as they both resist and reinforce heteronormative adulthood. My textual analysis of these films are grounded in theories of queer temporality. Finally, I focus on the female counterpart to the immature male, examining various constructions of female immaturity in recent cinema. Here, I utilize a broader range of queer temporal theories to demonstrate the political potential of immature womanhood, where “immature” gains agency through its queer and feminist resistance to the tyranny of heteronormative adulthood. While this dissertation ultimately seeks to demonstrate how immaturity functions as a site of resistance to (hetero)normativity, it also acknowledges how it can (specifically in the context of male homosociality), reinforce and reproduce oppressive structures, further underlining immaturity’s incongruous status.