scholarly journals Autologous Cell Seeding in Tracheal Tissue Engineering

2017 ◽  
Vol 3 (4) ◽  
pp. 279-289 ◽  
Author(s):  
Elizabeth F. Maughan ◽  
Robert E. Hynds ◽  
Toby J. Proctor ◽  
Sam M. Janes ◽  
Martin Elliott ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Seeding ◽  
Autologous Cell ◽  
Tracheal Tissue ◽  
Autologous Cell Seeding

Autologous cell seeding of safe bioscaffold prepared by ultrahigh pressure treatment

2004 ◽  
Vol 2004.16 (0) ◽  
pp. 435-436
Author(s):  
Toshia FUJISATO ◽  
Hiroshi NISHIOKA ◽  
Kazuo NIWAYA ◽  
Akio KISHIDA ◽  
Takeshi NAKATANI ◽  
...  
Keyword(s):  
Ultrahigh Pressure ◽  
Pressure Treatment ◽  
Cell Seeding ◽  
Autologous Cell ◽  
Autologous Cell Seeding

scholarly journals Patch augmentation of the pulmonary artery with bioabsorbable polymers and autologous cell seeding

2000 ◽  
Vol 120 (6) ◽  
pp. 1158-1167 ◽  
Author(s):  
Ulrich A. Stock ◽  
Takahiko Sakamoto ◽  
Shinichi Hatsuoka ◽  
David P. Martin ◽  
Mitsugi Nagashima ◽  
...  
Keyword(s):  
Pulmonary Artery ◽  
Cell Seeding ◽  
Autologous Cell ◽  
Autologous Cell Seeding

Nonwoven polycaprolactone scaffolds for tissue engineering: the choice of the structure and the method of cell seeding

2017 ◽  
Author(s):  
I. Arutyunyan ◽  
T. Tenchurin ◽  
E. Kananykhina ◽  
V. Chernikov ◽  
O. Vasyukova ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Seeding

3D bioprinting for lung and tracheal tissue engineering: Criteria, advances, challenges, and future directions

Bioprinting ◽  
2021 ◽  
Vol 21 ◽  
pp. e00124
Author(s):  
Seyed Hossein Mahfouzi ◽  
Seyed Hamid Safiabadi Tali ◽  
Ghassem Amoabediny
Keyword(s):  
Tissue Engineering ◽  
3D Bioprinting ◽  
Future Directions ◽  
Tracheal Tissue

Fabrication and characterization of customized tubular scaffolds for tracheal tissue engineering by using solvent based 3D printing on predefined template

2021 ◽  
Vol 27 (2) ◽  
pp. 421-428
Author(s):  
Rudranarayan Kandi ◽  
Pulak Mohan Pandey ◽  
Misba Majood ◽  
Sujata Mohanty
Keyword(s):  
Tissue Engineering ◽  
Cell Adhesion ◽  
3D Printing ◽  
Chemical Properties ◽  
Contact Angle Measurement ◽  
Angle Measurement ◽  
In Vitro Cytotoxicity ◽  
Content Type ◽  
Tracheal Tissue

Purpose This paper aims to discuss the successful fabrication of customized tubular scaffolds for tracheal tissue engineering with a novel route using solvent-based extrusion 3D printing. Design/methodology/approach The manufacturing approach involved extrusion of polymeric ink over a rotating predefined pattern to construct customized tubular structure of polycaprolactone (PCL) and polyurethane (PU). Dimensional deviation in thickness of scaffolds were calculated for various layer thicknesses of 3D printing. Physical and chemical properties of scaffolds were investigated by scanning electron microscope (SEM), contact angle measurement, Fourier Transform Infrared Spectroscopy (FTIR) and X-ray diffraction (XRD). Mechanical characterizations were performed, and the results were compared to the reported properties of human native trachea from previous reports. Additionally, in vitro cytotoxicity of the fabricated scaffolds was studied in terms of cell proliferation, cell adhesion and hemagglutination assay. Findings The developed fabrication route was flexible and accurate by printing customized tubular scaffolds of various scales. Physiochemical results showed good miscibility of PCL/PU blend, and decrease in crystalline nature of blend with the addition of PU. Preliminary mechanical assessments illustrated comparable mechanical properties with the native human trachea. Longitudinal compression test reported outstanding strength and flexibility to maintain an unobstructed lumen, necessary for the patency. Furthermore, the scaffolds were found to be biocompatible to promote cell adhesion and proliferation from the in vitro cytotoxicity results. Practical implications The attempt can potentially meet the demand for flexible tubular scaffolds that ease the concerns such as availability of suitable organ donors. Originality/value 3D printing over accurate predefined templates to fabricate customized grafts gives novelty to the present method. Various customized scaffolds were compared with conventional cylindrical scaffold in terms of flexibility.

Tracheal tissue-engineering: in-vivo biocompatibility of mechanically-stripped allogenic rabbit trachea with autologous epithelial covering

2016 ◽  
Vol 116 (3) ◽  
pp. 164-174 ◽  
Author(s):  
Margot Den Hondt ◽  
Bart M. Vanaudenaerde ◽  
Erik K. Verbeken ◽  
Jan J. Vranckx
Keyword(s):  
Tissue Engineering ◽  
Tracheal Tissue
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