Statistical Optimization of Xylanase from Bacillus licheniformis Using Banana Peels in Submerged Fermentation

Author(s):  
Hafiz Abdullah Shakir ◽  
Arfa Anwar ◽  
Muhammad Irfan ◽  
Muhammad Khan ◽  
Shaukat Ali ◽  
...  
2018 ◽  
Vol 15 (4) ◽  
pp. 887-898
Author(s):  
V. Antony Jenila ◽  
J. Joel Gnanadoss

Endophytic fungi Fusarium sp. LCJ273 capable of producing L-asparaginase was isolated from the medicinal plant Adhatoda vasica. The aim of the present study was to maximize L-asparaginase production by submerged fermentation through statistical optimization. L-Asparaginase production by Fusarium sp. LCJ273 was studied in five different media. Various nutritional parameters specifically carbon, nitrogen and inducers were optimized for enhancing the production of L-asparaginase. In addition, different statistical based experimental designs were also applied to increase the production of L-asparaginase by Fusarium sp. LCJ273. Dextrose, ammonium sulphate and wheat bran were found to be effective for growth and higher yield of L-asparaginase in Modified Czapek’s Dox Broth. Dextrose at a concentration of 3.0 g/L increased L-asparaginase production up to 9.18±0.9 U/mL, ammonium sulphate at the concentration of 20 g/L showed maximum L-asparaginase production up to 13.69±0.4 U/mL and wheat bran at 2.5 g/L yielded up to 14.24±0.5 U/mL. The maximum L-asparaginase production was observed by Fusarium sp. LCJ273 on 5th day. The study revealed that through optimization, a 2 fold increase in L-asparaginase could be achieved.


2020 ◽  
Vol 491 ◽  
pp. 107979 ◽  
Author(s):  
Zabin K. Bagewadi ◽  
Jayateerth S. Bhavikatti ◽  
Uday M. Muddapur ◽  
Deepak A. Yaraguppi ◽  
Sikandar I. Mulla

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