Prokaryotic community diversity during bioremediation of crude oil contaminated oilfield soil: effects of hydrocarbon concentration and salinity

2021 ◽  
Author(s):  
Celia Marcela Camacho-Montealegre ◽  
Edmo Montes Rodrigues ◽  
Daniel Kumazawa Morais ◽  
Marcos Rogério Tótola
Keyword(s):  
Crude Oil ◽  
Community Diversity ◽  
Prokaryotic Community ◽  
Hydrocarbon Concentration ◽  
Soil Effects

scholarly journals Prokaryotic Community Analysis of Crude Oil Artesian Area and Cultivation of Indigenous Aerobic-Anaerobic Petroleum Degrading Microorganisms

2017 ◽  
Vol 73 (7) ◽  
pp. III_165-III_173
Author(s):  
Akinori IGUCHI ◽  
Ryota MORI ◽  
Koshiro SUZUKI ◽  
Kentaro HASHIMOTO ◽  
Kazuki NOMURA ◽  
...  
Keyword(s):  
Crude Oil ◽  
Community Analysis ◽  
Prokaryotic Community

Archaeal diversity revealed in Antarctic sea ice

2011 ◽  
Vol 23 (6) ◽  
pp. 531-536 ◽  
Author(s):  
Rebecca O.M. Cowie ◽  
Elizabeth W. Maas ◽  
Ken G. Ryan
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Sea Ice ◽  
Community Diversity ◽  
Rrna Gene ◽  
Archaeal Diversity ◽  
Prokaryotic Community ◽  
Antarctic Sea Ice ◽  
Pcr Products ◽  
Global Biogeochemical Cycles

AbstractArchaea, once thought to be only extremophiles, are now known to be abundant in most environments. They can predominate in microbial communities and be significantly involved in many global biogeochemical cycles. However, Archaea have not been reported in Antarctic sea ice. Our understanding of the ecology of Antarctic sea ice prokaryotes is still in its infancy but this information is important if we are to understand their diversity, adaptations and biogeochemical roles in Antarctic systems. We detected Archaea in sea ice at two sampling sites taken from three subsequent years using conserved 16S rRNA gene archaeal primers and PCR. Archaeal abundance was measured using quantitative PCR and community diversity was investigated by sequencing cloned 16S rRNA gene PCR products. Archaea in Antarctic sea ice were found to be in low abundance consisting of ≤ 6.6% of the prokaryotic community. The majority, 90.8% of the sequences, clustered with the recently described phylumThaumarchaeota, one group closely clustered with the ammonia-oxidizing CandidatusNitrosopumilus maritimus. The remainder of the clones grouped with theEuryarchaeota.

Prokaryotic Community Diversity Along an Increasing Salt Gradient in a Soda Ash Concentration Pond

2015 ◽  
Vol 71 (2) ◽  
pp. 326-338 ◽  
Author(s):  
Addis Simachew ◽  
Anders Lanzén ◽  
Amare Gessesse ◽  
Lise Øvreås
Keyword(s):  
Community Diversity ◽  
Prokaryotic Community ◽  
Salt Gradient ◽  
Soda Ash

scholarly journals Response and oil degradation activities of a northeast Atlantic bacterial community to biogenic and synthetic surfactants

Microbiome ◽  
2021 ◽  
Vol 9 (1) ◽  
Author(s):  
Christina N. Nikolova ◽  
Umer Zeeshan Ijaz ◽  
Clayton Magill ◽  
Sara Kleindienst ◽  
Samantha B. Joye ◽  
...  
Keyword(s):  
Microbial Community ◽  
Crude Oil ◽  
Functional Diversity ◽  
Community Dynamics ◽  
Negative Impact ◽  
Community Diversity ◽  
Oil Degradation ◽  
Northeast Atlantic ◽  
Oil Biodegradation ◽  
Degrading Bacteria

Abstract Background Biosurfactants are naturally derived products that play a similar role to synthetic dispersants in oil spill response but are easily biodegradable and less toxic. Using a combination of analytical chemistry, 16S rRNA amplicon sequencing and simulation-based approaches, this study investigated the microbial community dynamics, ecological drivers, functional diversity and robustness, and oil biodegradation potential of a northeast Atlantic marine microbial community to crude oil when exposed to rhamnolipid or synthetic dispersant Finasol OSR52. Results Psychrophilic Colwellia and Oleispira dominated the community in both the rhamnolipid and Finasol OSR52 treatments initially but later community structure across treatments diverged significantly: Rhodobacteraceae and Vibrio dominated the Finasol-amended treatment, whereas Colwellia, Oleispira, and later Cycloclasticus and Alcanivorax, dominated the rhamnolipid-amended treatment. Key aromatic hydrocarbon-degrading bacteria, like Cycloclasticus, was not observed in the Finasol treatment but it was abundant in the oil-only and rhamnolipid-amended treatments. Overall, Finasol had a significant negative impact on the community diversity, weakened the taxa-functional robustness of the community, and caused a stronger environmental filtering, more so than oil-only and rhamnolipid-amended oil treatments. Rhamnolipid-amended and oil-only treatments had the highest functional diversity, however, the overall oil biodegradation was greater in the Finasol treatment, but aromatic biodegradation was highest in the rhamnolipid treatment. Conclusion Overall, the natural marine microbial community in the northeast Atlantic responded differently to crude oil dispersed with either synthetic or biogenic surfactants over time, but oil degradation was more enhanced by the synthetic dispersant. Collectively, our results advance the understanding of how rhamnolipid biosurfactants and synthetic dispersant Finasol affect the natural marine microbial community in the FSC, supporting their potential application in oil spills.

Prokaryotic community diversity in the sediments of saline lagoons and its resistance to seasonal disturbances by water level cycles

2021 ◽  
Author(s):  
Luis E. Sáenz de Miera ◽  
Juan J. Gutiérrez-González ◽  
Paula Arroyo ◽  
Jorge Falagán ◽  
Gemma Ansola
Keyword(s):  
Water Level ◽  
Community Diversity ◽  
Prokaryotic Community

scholarly journals Effect of Pyocyanin on a Crude-Oil-Degrading Microbial Community

2004 ◽  
Vol 70 (7) ◽  
pp. 4004-4011 ◽  
Author(s):  
R. Sean Norman ◽  
Peter Moeller ◽  
Thomas J. McDonald ◽  
Pamela J. Morris
Keyword(s):  
Superoxide Dismutase ◽  
Microbial Community ◽  
Crude Oil ◽  
Pure Culture ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Community Diversity ◽  
Interspecies Interactions ◽  
Polycyclic Aromatic ◽  
Gradient Gel Electrophoresis ◽  
High Level

ABSTRACT Pseudomonas aeruginosa is an n-alkane degrader that is frequently isolated from petroleum-contaminated sites and produces factors that enhance its competitiveness and survival in many environments. In this study, one such factor, pyocyanin, has been detected in an oil-degrading culture containing P. aeruginosa and is a redox-active compound capable of inhibiting microbial growth. To examine the effects of pyocyanin further, an oil-degrading culture was grown with and without 9.5 μM pyocyanin and microbial community structure and oil degradation were monitored for 50 days. Denaturing gradient gel electrophoresis (DGGE) analysis of cultures revealed a decrease in the microbial community diversity in the pyocyanin-amended cultures compared to that of the unamended cultures. Two members of the microbial community in pure culture exhibited intermediate and high sensitivities to pyocyanin corresponding to intermediate and low levels of activity for the antioxidant enzymes catalase and superoxide dismutase, respectively. Another member of the community that remained constant in the DGGE gels over the 50-day culture incubation period exhibited no sensitivity to pyocyanin, corresponding to a high level of catalase and superoxide dismutase when examined in pure culture. Pyocyanin also affected the overall degradation of the crude oil. At 50 days, the culture without pyocyanin had decreased polycyclic aromatic hydrocarbons compared to the pyocyanin-amended culture, with a specific reduction in the degradation of dibenzothiophenes, naphthalenes, and C29 and C30 hopanes. This study demonstrated that pyocyanin influenced the diversity of the microbial community and suggests the importance of understanding how interspecies interactions influence the degradation capability of a microbial community.

scholarly journals Response and Oil Degradation Activities of a Northeast Atlantic Bacterial Community to Biogenic and Synthetic Surfactants

2021 ◽  
Author(s):  
Christina Nikolova ◽  
Umer Zeeshan Ijaz ◽  
Clayton Magill ◽  
Sara Kleindienst ◽  
Samantha B. Joye ◽  
...  
Keyword(s):  
Microbial Community ◽  
Crude Oil ◽  
Functional Diversity ◽  
Community Dynamics ◽  
Negative Impact ◽  
Community Diversity ◽  
Oil Degradation ◽  
Northeast Atlantic ◽  
Oil Biodegradation ◽  
Degrading Bacteria

Abstract Background: Biosurfactants, however, are naturally derived products that play a similar role to synthetic dispersants in oil spill response but are easily biodegradable and less toxic. Using a combination of analytical chemistry, 16S rRNA amplicon sequencing and simulation-based approaches, this study investigated the microbial community dynamics, ecological drivers, functional diversity and robustness, and oil biodegradation potential of a northeast Atlantic marine microbial community to crude oil when exposed to rhamnolipid or synthetic dispersant Finasol OSR52. Results: Psychrophilic Colwellia and Oleispira dominated the community in both the rhamnolipid and Finasol OSR52 treatments initially but later community structure across treatments diverged significantly: Rhodobacteraceae and Vibrio dominated the Finasol-amended treatment, whereas Colwellia, Oleispira, and later Cycloclasticus and Alcanivorax, dominated the rhamnolipid-amended treatment. The key aromatic hydrocarbon-degrading bacteria like Cycloclasticus was not observed in the Finasol treatment but it was abundant in the oil-only and rhamnolipid-amended treatments. Overall, Finasol had a significant negative impact on the community diversity, weakened the taxa-functional robustness of the community, and caused a stronger environmental filtering, more so than oil-only and rhamnolipid-amended oil treatments. Rhamnolipid-amended and oil-only treatments had the highest functional diversity, however, the overall oil biodegradation was greater in the Finasol treatment, but aromatic biodegradation was highest in the rhamnolipid treatment. Conclusion: Overall, the natural marine microbial community in the northeast Atlantic responded differently to crude oil dispersed with either synthetic or biogenic surfactants over time, but oil degradation was more enhanced by the synthetic dispersant. Collectively, our results advance the understanding of how rhamnolipid biosurfactants and synthetic dispersant Finasol affect the natural marine microbial community in the FSC, supporting their potential application in oil spills.

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