Interphase Cytogenetics

2012 ◽  
Keyword(s):  
Interphase Cytogenetics

Primary extramedullary plasmacytoma: high frequency of breaks involving 14q32 and deletions of 13q detected by interphase cytogenetics

2004 ◽  
Vol 200 (4) ◽  
pp. 344
Author(s):  
K. Bink ◽  
M. Kremer ◽  
G. Ott ◽  
K. Specht ◽  
S. Mandl-Weber ◽  
...  
Keyword(s):  
High Frequency ◽  
Extramedullary Plasmacytoma ◽  
Interphase Cytogenetics

Interphase Cytogenetics

2015 ◽  
pp. 1-3
Author(s):  
Thomas Ried
Keyword(s):  
Interphase Cytogenetics

Interphase Cytogenetics in Plants

2004 ◽  
pp. 267-274
Author(s):  
I. Schubert ◽  
Z. Jasencakova ◽  
A. Meister ◽  
P. Fransz ◽  
M. Lysak
Keyword(s):  
Interphase Cytogenetics

Predictive Role of Interphase Cytogenetics for Survival of Patients With Multiple Myeloma

2000 ◽  
Vol 18 (4) ◽  
pp. 804-804 ◽  
Author(s):  
Robert Königsberg ◽  
Niklas Zojer ◽  
Jutta Ackermann ◽  
Elisabeth Krömer ◽  
Harald Kittler ◽  
...  
Keyword(s):  
Multiple Myeloma ◽  
Prognostic Factors ◽  
Chromosomal Abnormalities ◽  
Prognostic Significance ◽  
Response To Treatment ◽  
Induction Treatment ◽  
Interphase Cytogenetics ◽  
True Incidence ◽  
Conventional Dose ◽  
Conventional Dose Chemotherapy

PURPOSE: Recent metaphase cytogenetic studies suggested that specific chromosomal abnormalities are of prognostic significance in patients with multiple myeloma (MM). Because the true incidence of chromosomal abnormalities in MM is much higher than that detected by metaphase analysis, we used interphase fluorescence in situ hybridization (FISH) to determine the prognostic value of specific chromosomal aberrations. PATIENTS AND METHODS: Bone marrow plasma cells from 89 previously untreated patients with MM were studied consecutively by FISH to detect the deletions of 13q14, 17p13, and 11q and the presence of t(11;14)(q13;q32). FISH results were analyzed in the context of clinical parameters (response to treatment and survival after conventional-dose chemotherapy), and a multivariate analysis of prognostic factors was performed. RESULTS: By FISH, the deletion of 13q14 occurred in 40 patients (44.9%), deletion of 17p13 in 22 (24.7%), and 11q abnormalities in 14 (15.7%; seven with t(11;14)). Deletions of 13q14 and 17p13 were associated with poor response to induction treatment (46.9% v 77.3% in those without deletions, P = .006 and 40.0% v 73.2%, P = .008, respectively) and short median overall survival (OS) time (24.2 v 88.1 months, P = .008 and 16.2 v 51.3 months, P = .008, respectively). Short median OS time was also observed for patients with 11q abnormalities (13.1 v 41.6 months, P = .02). According to the number of unfavorable cytogenetic features (deletion of 13q14, deletion of 17p13, and aberrations of 11q) that were present in each patient (0 v 1 v 2 or 3), patients with significantly different OS times could be discriminated from one another (102.4 v 29.6 v 13.9 months, P < .001, respectively). CONCLUSION: For patients with MM who were treated with conventional-dose chemotherapy, interphase FISH for 13q14, 17p13, and 11q provides prognostically relevant information in addition to that provided by standard prognostic factors. This observation may be considered for risk-adapted stratifications of MM patients in future clinical trials.

scholarly journals Detection of Chromosomal Changes by Interphase Cytogenetics in Biopsies of Recurrent Astrocytomas and Oligodendrogliomas

1997 ◽  
Vol 56 (10) ◽  
pp. 1125-1131 ◽  
Author(s):  
S. M. ROSSO ◽  
H. VAN DEKKEN ◽  
K. K. KRISHNADATH ◽  
J. C. ALERS ◽  
J. M. KROS
Keyword(s):  
Interphase Cytogenetics ◽  
Chromosomal Changes

scholarly journals Comparison of cytogenetics, interphase cytogenetics, and DNA flow cytometry in bone tumors

Cytometry ◽  
1996 ◽  
Vol 26 (3) ◽  
pp. 185-191 ◽  
Author(s):  
Maija Tarkkanen ◽  
Stig Nordling ◽  
Tom B�hling ◽  
Aarne Kivioja ◽  
Erkki Karaharju ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Bone Tumors ◽  
Dna Flow Cytometry ◽  
Interphase Cytogenetics

SEX CHROMOSOME DETERMINATION IN EXTRAGONADAL TERATOMAS BY INTERPHASE CYTOGENETICS: Clues to Histogenesis

1997 ◽  
Vol 17 (3) ◽  
pp. 401-412 ◽  
Author(s):  
Hubert Wagner ◽  
Gustavo Bruno Baretton ◽  
Karin Schneiderbanger ◽  
Andreas Karl Bise ◽  
Udo Lohrs
Keyword(s):  
Sex Chromosome ◽  
Interphase Cytogenetics

Gains of 2p involving the REL locus correlate with nuclear c-Rel protein accumulation in neoplastic cells of classical Hodgkin lymphoma

Blood ◽  
2003 ◽  
Vol 101 (9) ◽  
pp. 3681-3686 ◽  
Author(s):  
Thomas F. E. Barth ◽  
José I. Martin-Subero ◽  
Stefan Joos ◽  
Christiane K. Menz ◽  
Cornelia Hasel ◽  
...  
Keyword(s):  
Hodgkin Lymphoma ◽  
Comparative Genomic ◽  
Protein Accumulation ◽  
Nuclear Staining ◽  
Chromosome 2 ◽  
Classical Hodgkin Lymphoma ◽  
Interphase Cytogenetics ◽  
Copy Numbers ◽  
Structural Aberrations ◽  
Translocation Breakpoints

Structural aberrations of the short arm of chromosome 2, mostly resulting in gains of 2p13∼16, have recently been described as being highly recurrent in Hodgkin and Reed-Sternberg (HRS) cells of classical Hodgkin lymphoma (cHL). As these gains consistently lead to increased copy numbers of the REL oncogene locus, we investigated the expression of the c-Rel protein in a series of 30 cHL cases with known genomic REL status as determined by comparative genomic hybridization and interphase cytogenetics. Expression of the c-Rel protein was investigated in 26 biopsies by immunohistochemistry. Distinct patterns were observed in HRS cells with no staining, cytoplasmic, and/or nuclear staining for c-Rel. All 13 samples with additional copies of the REL locus displayed nuclear staining for c-Rel, while 13 cHL samples lacking chromosome 2 (2p) gains displayed a significantly lower proportion or complete absence of HRS cells with nuclear c-Rel expression. Detailed analysis using combined immunophenotyping and interphase cytogenetics of individual HRS cells demonstrated that REL gains correlated with the presence of nuclear c-Rel staining. Additionally, in 2 cHL samples with translocation breakpoints in 2p13∼16, nuclear staining of c-Rel was observed; in one of them the staining pattern was indicative of a truncated c-Rel protein. The correlation between structural aberrations involving the REL locus and nuclear c-Rel accumulation in HRS cells qualifies REL as a target gene of the frequent gains in 2p in cHL. The data suggest thatREL aberrations are a genetic mechanism contributing to constitutive nuclear factor (NF)–κB/Rel activation in cHL.

Interphase Cytogenetics of Gastric and Esophageal Adenocarcinomas

1993 ◽  
Vol 2 (1) ◽  
pp. 264-268
Author(s):  
Pulivarthi H. Rao ◽  
Susan Mathew ◽  
Gregory Lauwers ◽  
Eduardo Rodriguez ◽  
David P. Kelsen ◽  
...  
Keyword(s):  
Interphase Cytogenetics
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