Enzymatic determination of alcohol mixtures at the nanogram level by the stopped-flow technique

1993 ◽  
Vol 274 (1) ◽  
pp. 109-116 ◽  
Author(s):  
Eva Förster ◽  
Manuel Silva ◽  
Matthias Otto ◽  
Dolores Pérez-Bendito
Keyword(s):  
Stopped Flow ◽  
Enzymatic Determination ◽  
Alcohol Mixtures ◽  
Nanogram Level

Kinetic-enzymatic determination of oxalate in urine by flow-injection analysis with double stopped flow

1991 ◽  
Vol 242 ◽  
pp. 179-183 ◽  
Author(s):  
Juan A. Infantes ◽  
María Dolores Luque de Castro ◽  
Miguel Valcárcel
Keyword(s):  
Flow Injection ◽  
Flow Injection Analysis ◽  
Stopped Flow ◽  
Enzymatic Determination

Kinetic enzymatic determination of ethanol/methanol mixtures by the stopped-flow technique

Talanta ◽  
1993 ◽  
Vol 40 (6) ◽  
pp. 855-861 ◽  
Author(s):  
Eva Förster ◽  
Manuel Silva ◽  
Mathias Otto ◽  
Dolores Pérez-Bendito
Keyword(s):  
Stopped Flow ◽  
Enzymatic Determination

Enzymatic determination of free l-(−)-malic acid in must and wine by stopped-flow flow-injection analysis

1991 ◽  
Vol 247 (1) ◽  
pp. 61-65 ◽  
Author(s):  
C.Garcia De María ◽  
T.Manzano Muñoz ◽  
A.Alonso Mateos ◽  
L.García De María
Keyword(s):  
Flow Injection ◽  
Flow Injection Analysis ◽  
Malic Acid ◽  
Stopped Flow ◽  
Enzymatic Determination

Enzymatic Determination of Hydroxysteroids in Human Skin Surface Lipids1

1963 ◽  
Vol 41 (5) ◽  
pp. 265-268 ◽  
Author(s):  
Thomas J Cook ◽  
Allan L Lorincz ◽  
Alan R Spector
Keyword(s):  
Human Skin ◽  
Skin Surface ◽  
Enzymatic Determination

Enzymatic colorimetry of lecithin and sphingomyelin in aqueous solution.

1983 ◽  
Vol 29 (8) ◽  
pp. 1513-1517 ◽  
Author(s):  
M W McGowan ◽  
J D Artiss ◽  
B Zak
Keyword(s):  
Aqueous Solution ◽  
Hydrogen Peroxide ◽  
Amniotic Fluid ◽  
Enzymatic Reaction ◽  
Detergent Solution ◽  
Enzymatic Determination ◽  
Red Color ◽  
Hydrolysis Of ◽  
Zwitterionic Detergent

Abstract A procedure for the enzymatic determination of lecithin and sphingomyelin in aqueous solution is described. The phospholipids are first dissolved in chloroform:methanol (2:1 by vol), the solvent is evaporated, and the residue is redissolved in an aqueous zwitterionic detergent solution. The enzymatic reaction sequences of both assays involve hydrolysis of the phospholipids to produce choline, which is then oxidized to betaine, thus generating hydrogen peroxide. The hydrogen peroxide is subsequently utilized in the enzymatic coupling of 4-aminoantipyrine and sodium 2-hydroxy-3,5-dichlorobenzenesulfonate, an intensely red color being formed. The presence of a non-reacting phospholipid enhances the hydrolysis of the reacting phospholipid. Thus we added lecithin to the sphingomyelin standards and sphingomyelin to the lecithin standards. This precise procedure may be applicable to determination of lecithin and sphingomyelin in amniotic fluid.

Non-enzymatic determination of purine nucleotides using a carbon dot modified glassy carbon electrode

2019 ◽  
Vol 11 (30) ◽  
pp. 3866-3873 ◽  
Author(s):  
R. Karthikeyan ◽  
D. James Nelson ◽  
S. Abraham John
Keyword(s):  
Glassy Carbon ◽  
Low Cost ◽  
Phosphate Buffer Solution ◽  
Purine Nucleotides ◽  
Buffer Solution ◽  
Solution Ph ◽  
Carbon Dot ◽  
Enzymatic Determination ◽  
Sensitive Determination

Selective and sensitive determination of one of the purine nucleotides, inosine (INO) using a low cost carbon dot (CD) modified glassy carbon (GC) electrode in 0.2 M phosphate buffer solution (pH 7.2) was demonstrated in this paper.

Sign in / Sign up

Export Citation Format

Share Document