Determination of 35S-aminoacyl-transfer ribonucleic acid specific radioactivity in small tissue samples

1981 ◽  
Vol 118 (1) ◽  
pp. 155-161 ◽  
Author(s):  
Allen M. Samarel ◽  
Edward A. Ogunro ◽  
Alan G. Ferguson ◽  
Michael Lesch
Keyword(s):  
Ribonucleic Acid ◽  
Specific Radioactivity ◽  
Tissue Samples ◽  
Small Tissue ◽  
Aminoacyl Transfer

Determination of glycogen in small tissue samples.

1970 ◽  
Vol 28 (2) ◽  
pp. 234-236 ◽  
Author(s):  
S Lo ◽  
J C Russell ◽  
A W Taylor
Keyword(s):  
Tissue Samples ◽  
Small Tissue

Determination of the activity of diamine oxidase in extremely small tissue samples

1995 ◽  
Vol 44 (S1) ◽  
pp. S94-S95 ◽  
Author(s):  
H. G. Schwelberger ◽  
J. Klocker ◽  
J. Sattler ◽  
E. Bodner
Keyword(s):  
Diamine Oxidase ◽  
Tissue Samples ◽  
Small Tissue

scholarly journals Preparation of polyribosome aminoacyl-transfer ribonucleic acid from the muscle of chick embryos.

1975 ◽  
Vol 147 (3) ◽  
pp. 473-477 ◽  
Author(s):  
M Nwagwu
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Sodium Dodecyl Sulphate ◽  
Ribonucleic Acid ◽  
Free Amino Acids ◽  
Free Amino ◽  
Sodium Dodecyl ◽  
Specific Radioactivity ◽  
Chick Embryos ◽  
Aminoacyl Transfer

A procedure for preparing polyribosome aminoacyl-tRNA free from contamination by supernatant aminoacyl-tRNA and free amino acids is described. Important features of the procedure are the use of acidic buffers to help protect the amino acid-tRNA linkage and the inclusion of sodium dodecyl sulphate, to inhibit ribonuclease activity. The specific radioactivity of polyribosome aminoacyl-tRNA is high within 30s and reaches a maximum in 2 1/2 min, well ahead of polyribosome peptides which, as described by Herrmann et al. (1971), attain maximum specific radioactivity in about 10 min.

scholarly journals Anabolism versus catabolism of [5-3H]uridine and its relationship to ribonucleic acid labelling in mouse liver after partial hepatectomy

1979 ◽  
Vol 178 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Torsten Yngner ◽  
Claes Engelbrecht ◽  
Lillemor Lewan ◽  
Jan-Erik Annerfeldt
Keyword(s):  
Nucleic Acid ◽  
Partial Hepatectomy ◽  
Ribonucleic Acid ◽  
Mouse Liver ◽  
Rna Synthesis ◽  
Specific Radioactivity ◽  
Acid Synthesis ◽  
Liver Cells ◽  
Control Value

The balance between anabolism and catabolism of [5-3H]uridine was studied in the mouse after partial hepatectomy. Labelling of RNA and UDP-glucose was determined and evaluated in relation to changes in the specific radioactivity of UTP. The amounts of labelled catabolic products of uridine were increased several-fold in liver and blood after partial hepatectomy. The specific radioactivity of RNA decreased to about 60% of the control value at 6h and was in the same range as that of control liver at 24h after operation. Decreased labelling of RNA and UDP-glucose was attributable to decreased specific radioactivity of UTP. No changes in the size of the UTP pool or in the balance between uridine anabolism and catabolism were found that could explain the decreased specific radioactivity of UTP. Rather, the alterations in the labelling of this metabolite induced by the partial hepatectomy may be related to decreased phosphorylating capacity in the liver cells and/or dilution of the labelled precursor in an expanded uridine pool. The enhanced amounts of uridine catabolic products in liver and blood were probably a consequence of accumulation and altered incorporation of the metabolites from the blood into the liver cells. Despite the increased amounts of labelled catabolic products and the decreased labelling of RNA, the results reported here actually suggest decreased uridine catabolism and slightly increased RNA synthesis in mouse liver after partial hepatectomy. The results stress the importance of proper controls in determination of nucleic acid synthesis and in metabolic studies by use of labelled precursors.

scholarly journals Analysis of the specific radioactivity of valine isolated from aminoacyl-transfer ribonucleic acid of rat liver

1974 ◽  
Vol 140 (3) ◽  
pp. 545-548 ◽  
Author(s):  
C S Wallyn ◽  
A Vidrich ◽  
J Airhart ◽  
E A Khairallah
Keyword(s):  
Rat Liver ◽  
Ribonucleic Acid ◽  
Specific Radioactivity ◽  
Aminoacyl Transfer
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