Variables in the high-pressure cation-exchange chromatography of proteins

1982 ◽  
Vol 125 (1) ◽  
pp. 203-209 ◽  
Author(s):  
Charles A. Frolik ◽  
Linda L. Dart ◽  
Michael B. Sporn
Keyword(s):  
High Pressure ◽  
Cation Exchange ◽  
Exchange Chromatography ◽  
Cation Exchange Chromatography

The Determination of the DNA Base Composition in 19 Species of Adriatic Sponges with High-Pressure Liquid Cation-Exchange Chromatography

1976 ◽  
Vol 31 (9-10) ◽  
pp. 554-557 ◽  
Author(s):  
Hans-Joachim Breter ◽  
Ferdinand Hundt ◽  
Rudolf K. Zahn
Keyword(s):  
High Pressure ◽  
Cation Exchange ◽  
Base Composition ◽  
Exchange Chromatography ◽  
Cation Exchange Chromatography ◽  
Dna Base Composition ◽  
Dna Base ◽  
Semiquantitative Method ◽  
Adenine Thymine

Abstract The (adenine + thymine)/(guanine + cytosine) base ratios of 19 species of adriatic sponges have been determined by high-pressure liquid cation-exchange chromatography. The base ratios vary from 1.49 (Mycale massa) to 0.63 (Hippospongia communis) according to an (A+T) content of 59.7 and 38.6 mol%, respectively. The DNAs of sponges of the order Keratosa showed marked differences in their (A +T) contents (39.5 to 58.8 mol%) whereas those of Tetractinellida and Halichondrina were nearly identical (39.3 to 40.8 and 49.5 to 49.8 mol%, respectively). The 5-methylcytosine (5MC) content was determined in 8 sponge DNAs by a semiquantitative method. The values differed from 0.8 to 2.2 mol% of 5MC.

IV. An Improved Separation Method for Twenty two Compounds Related to Purine and 6-Thiopurine Metabolism Using High-Pressure Liquid Cation-Exchange Chromatography

1977 ◽  
Vol 32 (11-12) ◽  
pp. 905-907 ◽  
Author(s):  
Hans-Joachim Breter
Keyword(s):  
High Pressure ◽  
Cation Exchange ◽  
Flow Velocity ◽  
Exchange Resin ◽  
Exchange Chromatography ◽  
Separation Method ◽  
Cation Exchange Chromatography ◽  
Improved Method ◽  
Linear Flow ◽  
Linear Flow Velocity

Abstract An improved method is described for the separation of 22 compounds normally related to purine and 6-thiopurine metabolism in biological materials using high-pressure liquid cation-exchange chromatography on strongly acidic exchange resin. The column (0.18 × 100 cm) is eluted with 0.4 ᴍ ammonium formate, pH 4.6, at a linear flow velocity of 5.2 cm · min-1 at 50 °C. The elution volumes of sulphate anions, allopurinol, 6-thioxanthine, adenine, adenosine, and guanosine are demonstrated additionally to further 16 purine and 6-thiopurine compounds.

High pressure cation exchange chromatography of biogenic amines

1976 ◽  
Vol 72 (1-2) ◽  
pp. 566-572 ◽  
Author(s):  
Kenneth D. McMurtrey ◽  
Laurence R. Meyerson ◽  
Jesse L. Cashaw ◽  
Virginia E. Davis
Keyword(s):  
High Pressure ◽  
Cation Exchange ◽  
Biogenic Amines ◽  
Exchange Chromatography ◽  
Cation Exchange Chromatography
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