A semi-integrated method for the determination of enzyme kinetic parameters and graphical representation of the Michaelis-Menten equation

1984 ◽  
Vol 141 (1) ◽  
pp. 179-183 ◽  
Author(s):  
Ali Naqui ◽  
Britton Chance
Keyword(s):  
Kinetic Parameters ◽  
Graphical Representation ◽  
Enzyme Kinetic ◽  
Integrated Method

On linear concentration dependences of K'm, V' and 1/νi enzyme kinetic parameters

1990 ◽  
Vol 55 (5) ◽  
pp. 1351-1365 ◽  
Author(s):  
Vladimír I. Krupyanko
Keyword(s):  
Kinetic Parameters ◽  
Enzyme Inhibition ◽  
Enzyme Activation ◽  
Enzyme Kinetic ◽  
Concentration Dependences ◽  
Linear Concentration

The most frequently occurring incorrect uses of the plots of intercepts, slopes and other coordinates for the determination of constants of enzyme activation Ka and of nontrivial inhibition K(IIi), K(Vi), K(Ii), were analyzed. The correct plots are shown. Calculations of the K(Ii) and K(IIi) constants of nontrivial types of enzyme inhibition using the corrected plots of the intercepts are given.

Determination of Enzyme Kinetic Parameters of UDP-glycosyltransferases

BIO-PROTOCOL ◽  
2013 ◽  
Vol 3 (14) ◽  
Author(s):  
Jörg Augustin ◽  
Søren Bak
Keyword(s):  
Kinetic Parameters ◽  
Enzyme Kinetic

Influence of the experimental setup on the determination of enzyme kinetic parameters

2016 ◽  
Vol 33 (1) ◽  
pp. 87-95 ◽  
Author(s):  
Jan-Hendrik Grosch ◽  
David Wagner ◽  
Niklas Knaup ◽  
Timm Keil ◽  
Antje C. Spieß
Keyword(s):  
Kinetic Parameters ◽  
Experimental Setup ◽  
Enzyme Kinetic

A new multi-wavelength model-based method for determination of enzyme kinetic parameters

2010 ◽  
Vol 35 (3) ◽  
pp. 395-403 ◽  
Author(s):  
Mohammad-Hossein Sorouraddin ◽  
Kaveh Amini ◽  
Abdolhossein Naseri ◽  
Javad Vallipour ◽  
Jalal Hanaee ◽  
...  
Keyword(s):  
Kinetic Parameters ◽  
Enzyme Kinetic ◽  
Model Based ◽  
Multi Wavelength

What students must know about the determination of enzyme kinetic parameters

1999 ◽  
Vol 27 (2) ◽  
pp. 87-91 ◽  
Author(s):  
Francesco Ranaldi ◽  
Paolo Vanni ◽  
Eugenio Giachetti
Keyword(s):  
Kinetic Parameters ◽  
Enzyme Kinetic

scholarly journals Evaluation of Enzyme Kinetic Parameters to Produce Methanol Using Michaelis-Menten Equation

2019 ◽  
Vol 14 (2) ◽  
pp. 436
Author(s):  
Norazwina Zainol ◽  
Siti Natrah Ismail
Keyword(s):  
Kinetic Parameters ◽  
Kinetic Constant ◽  
Time Range ◽  
Enzyme Kinetic ◽  
Methanol Production ◽  
Biotechnology Research ◽  
Runge Kutta Method ◽  
Runge Kutta ◽  
Hydrolysis Of

Determination of kinetic parameters of enzymes is important in biotechnology research. It is also one of the most challenging processes in methanol production. The activity of enzyme is determined in term of initial rates at various substrate concentrations. The enzymatic hydrolysis of methanol by pectin methyl esterase (PME) enzyme was investigated at 25 °C and pH 9 over the reaction time range from 0 to 90 min. In this study, the parameters of the enzyme's kinetic, KM and Vmax were directly determined using a modified Michaelis-Menten equation by applying the Lineweaver-Burk plots. Besides, nonlinear regression of Michaelis-Menten equation was calculated based on Euler’s and Runge-Kutta 4th order methods by using Solver supplement application. The result of kinetic constant was tested by comparing the experimental data with model predictions. It was found that Euler and Runge-Kutta method was successful in determining the kinetic parameter rather than Lineweaver-Burk plot. The application of the Michaelis-Menten equation describes the enzyme kinetic very well. From the kinetic analysis, it showed the good agreement between the result obtained and the predictions model in the production of methanol using PME enzyme. Copyright © 2019 BCREC Group. All rights reserved 

Determination of enzyme kinetic parameters of cyclic CMP-specific phosphodiesterase by quantitative fast atom bombardment tandem mass spectrometry

1991 ◽  
Vol 26 (5) ◽  
pp. 447-453 ◽  
Author(s):  
Russell P. Newton ◽  
Jalal A. Khan ◽  
Dipankar Ghosh ◽  
James I. Langridge ◽  
A. Gareth Brenton ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Kinetic Parameters ◽  
Fast Atom Bombardment ◽  
Tandem Mass ◽  
Enzyme Kinetic ◽  
Cyclic Cmp
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