Determination of sulfhydryl groups and disulfide bonds in a protein by polyacrylamide gel electrophoresis

1990 ◽  
Vol 188 (2) ◽  
pp. 359-365 ◽  
Author(s):  
Nobuyuki Takahashi ◽  
Masaaki Hirose
Keyword(s):  
Gel Electrophoresis ◽  
Disulfide Bonds ◽  
Polyacrylamide Gel Electrophoresis ◽  
Polyacrylamide Gel ◽  
Sulfhydryl Groups

Urease. X. A study by gel electrophoresis of its dissociation by sodium dodecyl sulfate

1969 ◽  
Vol 47 (10) ◽  
pp. 989-991 ◽  
Author(s):  
D. P. Blattler ◽  
George Gorin
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Gel Electrophoresis ◽  
Disulfide Bonds ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Polyacrylamide Gel ◽  
Dodecyl Sulfate ◽  
Polypeptide Chains

Urease, m.w. 480 000, treated with an excess of sodium dodecyl sulfate is converted to a product of greatly increased mobility in polyacrylamide gel electrophoresis. We estimate its weight to be about 80 000. Treatment with excess thiol and detergent yielded the same product as detergent alone, indicating that the subunit or subunits do not contain polypeptide chains linked by disulfide bonds.

Size Determination of Gold Clusters by Polyacrylamide Gel Electrophoresis in a Large Cluster Region

2009 ◽  
Vol 113 (32) ◽  
pp. 14076-14082 ◽  
Author(s):  
Keisaku Kimura ◽  
Nobuyuki Sugimoto ◽  
Seiichi Sato ◽  
Hiroshi Yao ◽  
Yuichi Negishi ◽  
...  
Keyword(s):  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Gold Clusters ◽  
Polyacrylamide Gel ◽  
Large Cluster ◽  
Size Determination ◽  
Cluster Region
Sign in / Sign up

Export Citation Format

Share Document