Large-scale cell separation by centrifugal elutriation

1992 ◽  
Vol 200 (2) ◽  
pp. 376-380 ◽  
Author(s):  
M.P. Lutz ◽  
G. Gaedicke ◽  
W. Hartmann
Keyword(s):  
Cell Separation ◽  
Large Scale ◽  
Centrifugal Elutriation

Development and study of a method for cell separation during white blood cell segmentation on images of bone marrow preparations in information and measurement systems for diagnostics of acute leukemia

2020 ◽  
pp. 68-72
Author(s):  
V.G. Nikitaev ◽  
A.N. Pronichev ◽  
V.V. Dmitrieva ◽  
E.V. Polyakov ◽  
A.D. Samsonova ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Blood Cell ◽  
Acute Leukemia ◽  
White Blood Cell ◽  
Cell Separation ◽  
Large Scale ◽  
Bone Marrow Cells ◽  
Automated Analysis ◽  
Cell Segmentation ◽  
Measurement Systems

The issues of using of information and measurement systems based on processing of digital images of microscopic preparations for solving large-scale tasks of automating the diagnosis of acute leukemia are considered. The high density of leukocyte cells in the preparation (hypercellularity) is a feature of microscopic images of bone marrow preparations. It causes the proximity of cells to eachother and their contact with the formation of conglomerates. Measuring of the characteristics of bone marrow cells in such conditions leads to unacceptable errors (more than 50%). The work is devoted to segmentation of contiguous cells in images of bone marrow preparations. A method of cell separation during white blood cell segmentation on images of bone marrow preparations under conditions of hypercellularity of the preparation has been developed. The peculiarity of the proposed method is the use of an approach to segmentation of cell images based on the watershed method with markers. Key stages of the method: the formation of initial markers and builds the lines of watershed, a threshold binarization, shading inside the outline. The parameters of the separation of contiguous cells are determined. The experiment confirmed the effectiveness of the proposed method. The relative segmentation error was 5 %. The use of the proposed method in information and measurement systems of computer microscopy for automated analysis of bone marrow preparations will help to improve the accuracy of diagnosis of acute leukemia.

Implementation of a semiclosed large scale counterflow centrifugal elutriation system

1987 ◽  
Vol 3 (3) ◽  
pp. 154-160 ◽  
Author(s):  
Ino K. Gao ◽  
Stephen J. Noga ◽  
John E. Wagner ◽  
Carol A. Cremo ◽  
Janice Davis ◽  
...  
Keyword(s):  
Large Scale ◽  
Centrifugal Elutriation

Stimulatory effect of counterflow centrifugal elutriation in large-scale separation of peripheral blood monocytes can be reversed by storing the cells at 37°c

1991 ◽  
Vol 6 (1) ◽  
pp. 48-53 ◽  
Author(s):  
Tore G. Abrahamsen ◽  
Charles S. Carter ◽  
Elizabeth J. Read ◽  
Marc Rubin ◽  
Holly G. Goetzman ◽  
...  
Keyword(s):  
Peripheral Blood ◽  
Large Scale ◽  
Centrifugal Elutriation ◽  
Blood Monocytes ◽  
Scale Separation ◽  
Peripheral Blood Monocytes

Microfluidic counterflow centrifugal elutriation system for sedimentation-based cell separation

2012 ◽  
Vol 14 (6) ◽  
pp. 1049-1057 ◽  
Author(s):  
Tomoki Morijiri ◽  
Masumi Yamada ◽  
Toshikatsu Hikida ◽  
Minoru Seki
Keyword(s):  
Cell Separation ◽  
Centrifugal Elutriation

CELL SEPARATION BY COUNTERCURRENT CENTRIFUGAL ELUTRIATION: RECENT DEVELOPMENTS

2012 ◽  
Vol 42 (3) ◽  
pp. 217-233 ◽  
Author(s):  
Jirka Grosse ◽  
Karl Meier ◽  
Thomas J. Bauer ◽  
Christoph Eilles ◽  
Daniela Grimm
Keyword(s):  
Cell Separation ◽  
Centrifugal Elutriation ◽  
Recent Developments

Some comments about observations and image processing of comet 29P/Schwassmann-Wachmann 1

1999 ◽  
Vol 173 ◽  
pp. 243-248
Author(s):  
D. Kubáček ◽  
A. Galád ◽  
A. Pravda
Keyword(s):  
Image Processing ◽  
Large Scale ◽  
Harvard College ◽  
Oak Ridge ◽  
Large Scale Structures ◽  
Short Period Comet ◽  
Short Period ◽  
Scale Structures ◽  
Harvard College Observatory ◽  
Period Comet

AbstractUnusual short-period comet 29P/Schwassmann-Wachmann 1 inspired many observers to explain its unpredictable outbursts. In this paper large scale structures and features from the inner part of the coma in time periods around outbursts are studied. CCD images were taken at Whipple Observatory, Mt. Hopkins, in 1989 and at Astronomical Observatory, Modra, from 1995 to 1998. Photographic plates of the comet were taken at Harvard College Observatory, Oak Ridge, from 1974 to 1982. The latter were digitized at first to apply the same techniques of image processing for optimizing the visibility of features in the coma during outbursts. Outbursts and coma structures show various shapes.

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