Primary-charge separation and excitation of chlorophyll a in photosystem II particles from spinach as studied by picosecond absorbance-difference spectroscopy

1986 ◽  
Vol 848 (2) ◽  
pp. 167-175 ◽  
Author(s):  
Antonius M. Nuijs ◽  
Hans J. van Gorkom ◽  
Johan J. Plijter ◽  
Louis N.M. Duysens
Keyword(s):  
Photosystem Ii ◽  
Chlorophyll A ◽  
Charge Separation ◽  
Difference Spectroscopy ◽  
Primary Charge Separation ◽  
Absorbance Difference ◽  
Photosystem Ii Particles

Determination of the primary charge separation rate in Photosystem II reaction centers at 15 K

1989 ◽  
Vol 22 (1) ◽  
pp. 89-99 ◽  
Author(s):  
Michael R. Wasielewski ◽  
Douglas G. Johnson ◽  
Govindjee ◽  
Christopher Preston ◽  
Michael Seibert
Keyword(s):  
Photosystem Ii ◽  
Charge Separation ◽  
Reaction Centers ◽  
Primary Charge Separation ◽  
Separation Rate

scholarly journals Site-Directed Mutations at D1-His198 and D2-His197 of Photosystem II inSynechocystisPCC 6803:  Sites of Primary Charge Separation and Cation and Triplet Stabilization†

Biochemistry ◽  
2001 ◽  
Vol 40 (31) ◽  
pp. 9265-9281 ◽  
Author(s):  
Bruce A. Diner ◽  
Eberhard Schlodder ◽  
Peter J. Nixon ◽  
William J. Coleman ◽  
Fabrice Rappaport ◽  
...  
Keyword(s):  
Photosystem Ii ◽  
Charge Separation ◽  
Primary Charge Separation

scholarly journals The formation of the split EPR signal from the S3 state of Photosystem II does not involve primary charge separation

2011 ◽  
Vol 1807 (1) ◽  
pp. 11-21 ◽  
Author(s):  
Kajsa G.V. Havelius ◽  
Ji-Hu Su ◽  
Guangye Han ◽  
Fikret Mamedov ◽  
Felix M. Ho ◽  
...  
Keyword(s):  
Photosystem Ii ◽  
Charge Separation ◽  
Primary Charge Separation ◽  
Epr Signal

scholarly journals Properties of oxygen-evolving photosystem-II particles from Phormidium laminosum, a thermophilic blue-green alga

1981 ◽  
Vol 194 (3) ◽  
pp. 877-887 ◽  
Author(s):  
A C Stewart ◽  
D S Bendall
Keyword(s):  
Photosystem Ii ◽  
Chlorophyll A ◽  
Green Alga ◽  
Fluorescence Emission ◽  
O2 Evolution ◽  
Fluorescence Emission Spectrum ◽  
Blue Green Alga ◽  
Photosynthetic Unit ◽  
Phormidium Laminosum ◽  
Photosystem Ii Particles

1. O2-evolving Photosystem-II particles from the thermophilic blue–green alga Phormidium laminosum contained 1 mol of Mn/13–17 mol of chlorophyll a compared with 1 mol of Mn/65–75 mol of chlorophyll a in unfractionated membranes. 2. At least two-thirds of the Mn in the Photosystem-II particles was removed by mild heating and by treatment with Tris or EDTA, with concomitant loss of O2 evolution. However, irreversible inactivation was also caused by washing in buffers without MgCl2, and this inactivation was not accompanied by a corresponding loss of Mn. 3. Bivalent cations (Mg2+ or Ca2+), Cl- or Br- ions and at least 20% (v/v) glycerol were required for maximum stability of O2 evolution. 4. The Photosystem-II particles were enriched in high-potential cytochrome b-559 (1 mol of cytochrome/50–60 mol of chlorophyll a) and in component C-550, and had a photosynthetic-unit size of 40–70 molecules of chlorophyll a. 5. The absorption spectrum at 77 K showed a preponderance of shorter-wavelength forms of chlorophyll a in the Photosystem-II particles, and in the fluorescence emission spectrum at 77 K there were major chlorophyll fluorescence bands at 684 nm and 695 nm, with almost no fluorescence in the far-red region. 6. Analysis of the lipid and protein contents showed that the Photosystem-II particles were not chemically pure (for example, all of the membrane-bound cytochromes and cytochrome c-549 were present), but their high O2-evolution activity and good optical properties make them useful for functional studies on Photosystem-II and O2 evolution.

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