Relative degradation of different arachidonoyl molecular species of choline glycerophospholipids in opsonized zymosan-stimulated rabbit alveolar macrophages

1986 ◽  
Vol 876 (3) ◽  
pp. 601-610 ◽  
Author(s):  
Nakagawa Yasuhito ◽  
Kurihara Koichi ◽  
Sugiura Takayuki ◽  
Waku Keizo
Keyword(s):  
Molecular Species ◽  
Alveolar Macrophages ◽  
Opsonized Zymosan ◽  
Choline Glycerophospholipids

scholarly journals Oestradiol-induced changes in the composition of phospholipid classes of quail oviduct: specific replacement of arachidonic acid by docosahexaenoic acid in alkenylacyl-glycerophosphoethanolamine

1994 ◽  
Vol 301 (2) ◽  
pp. 361-366 ◽  
Author(s):  
B E Felouati ◽  
J F Pageaux ◽  
J M Fayard ◽  
M Lagarde ◽  
C Laugier
Keyword(s):  
Cell Proliferation ◽  
Arachidonic Acid ◽  
Molecular Species ◽  
Phospholipid Composition ◽  
Oestrogen Treatment ◽  
Ethanolamine Glycerophospholipids ◽  
Striking Result ◽  
Choline Glycerophospholipids ◽  
Phospholipid Classes ◽  
Induced Changes

The phospholipid composition and the molecular species of the major subclasses of ethanolamine and choline glycerophospholipids were determined during the natural or oestradiol-induced development of the quail oviduct. The phospholipid concentration increased significantly during oviduct development, and the proportion of ethanolamine glycerophospholipids (EPL) remained constant while that of choline glycerophospholipids increased. The immature oviduct contained the majority of its endogenous arachidonic acid mass (71%) in EPL, mainly in alkenylacyl-glycerophosphoethanolamine (alkenylacyl-GPE) (49% of the total). Oestrogen treatment induced the depletion of 20:4,n-6 specifically from this pool, which indicates the biological importance of 20:4,n-6 molecular species in alkenylacyl-GPE as substrates for the oviduct phospholipases activated by oestradiol, and suggests that this EPL subclass is involved in the oestrogen-induced cell proliferation. Another striking result was the marked increase in 22:6,n-3 EPL molecular species following the oestradiol treatment and more particularly the strict substitution of 20:4,n-6 by 22:6,n-3 in alkenylacyl-GPE. We speculate that alkenylacyl-GPE molecular species containing 22:6,n-3 may participate in the arrest of oestrogen-induced proliferation.

scholarly journals Oxidative metabolic responses of rabbit pulmonary alveolar macrophages

Blood ◽  
1979 ◽  
Vol 53 (3) ◽  
pp. 486-491
Author(s):  
LA Boxer ◽  
G Ismail ◽  
JM Allen ◽  
RL Baehner
Keyword(s):  
Hydrogen Peroxide ◽  
Superoxide Dismutase ◽  
Alveolar Macrophages ◽  
Cytochalasin B ◽  
Surface Membrane ◽  
Nitroblue Tetrazolium ◽  
Hexose Monophosphate ◽  
Hexose Monophosphate Shunt ◽  
Opsonized Zymosan ◽  
Ferricytochrome C

During phagocytosis of opsonized lipopolysaccharide-coated paraffin oil droplets, rabbit alveolar macrophages reduced nitroblue tetrazolium, which effect was in part inhibitable with the use of superoxide dismutase. Exposure of cytochalasin-B-treated rabbit alveolar macrophages to opsonized zymosan led to the generation of superoxide, as quantitated by ferricytochrome C reduction. It was found that nitroblue tetrazolium in the presence of ferricytochrome C could in turn serve as scavenger of superoxide during stimulation of cytochalasin-B-treated rabbit alveolar macrophages. Following challenge with either opsonized zymosan or the membrane perturbant digitonin, rabbit alveolar macrophages released hydrogen peroxide into the extracellular medium. Employment of the surface membrane stimulant phorbol myristrate acetate led to activation of the hexose monophosphate shunt, which activity could be further enhanced in the presence of superoxide dismutase or attenuated in the presence of catalase. These studies demonstrate that rabbit alveolar macrophages release superoxide and hydrogen peroxide during surface membrane perturbation. In turn, hydrogen peroxide generation can stimulate the hexose monophosphate shunt.

Opsonized zymosan decreases cytoplasmic motility of alveolar macrophages in dogs

1992 ◽  
Vol 89 (2) ◽  
pp. 249-260 ◽  
Author(s):  
K. Zayasu ◽  
T. Fukushima ◽  
M. Yamaya ◽  
K. Sekizawa ◽  
K. Yamauchi ◽  
...  
Keyword(s):  
Alveolar Macrophages ◽  
Opsonized Zymosan

scholarly journals Oxidative metabolic responses of rabbit pulmonary alveolar macrophages

Blood ◽  
1979 ◽  
Vol 53 (3) ◽  
pp. 486-491 ◽  
Author(s):  
LA Boxer ◽  
G Ismail ◽  
JM Allen ◽  
RL Baehner
Keyword(s):  
Hydrogen Peroxide ◽  
Superoxide Dismutase ◽  
Alveolar Macrophages ◽  
Cytochalasin B ◽  
Surface Membrane ◽  
Nitroblue Tetrazolium ◽  
Hexose Monophosphate ◽  
Hexose Monophosphate Shunt ◽  
Opsonized Zymosan ◽  
Ferricytochrome C

Abstract During phagocytosis of opsonized lipopolysaccharide-coated paraffin oil droplets, rabbit alveolar macrophages reduced nitroblue tetrazolium, which effect was in part inhibitable with the use of superoxide dismutase. Exposure of cytochalasin-B-treated rabbit alveolar macrophages to opsonized zymosan led to the generation of superoxide, as quantitated by ferricytochrome C reduction. It was found that nitroblue tetrazolium in the presence of ferricytochrome C could in turn serve as scavenger of superoxide during stimulation of cytochalasin-B-treated rabbit alveolar macrophages. Following challenge with either opsonized zymosan or the membrane perturbant digitonin, rabbit alveolar macrophages released hydrogen peroxide into the extracellular medium. Employment of the surface membrane stimulant phorbol myristrate acetate led to activation of the hexose monophosphate shunt, which activity could be further enhanced in the presence of superoxide dismutase or attenuated in the presence of catalase. These studies demonstrate that rabbit alveolar macrophages release superoxide and hydrogen peroxide during surface membrane perturbation. In turn, hydrogen peroxide generation can stimulate the hexose monophosphate shunt.

Changes in molecular species of rat liver choline glycerophospholipids with development

1980 ◽  
Vol 618 (3) ◽  
pp. 431-438 ◽  
Author(s):  
Ogino Hirotaro ◽  
Matsumura Tadaki ◽  
Satouchi Kiyoshi ◽  
Saito Kunihiko
Keyword(s):  
Rat Liver ◽  
Molecular Species ◽  
Choline Glycerophospholipids
Sign in / Sign up

Export Citation Format

Share Document