Coordinate actions of arachidonic acid and protein kinase C in gonadotropin-releasing hormone-stimulated secretion of luteinizing hormone

1986 ◽  
Vol 134 (1) ◽  
pp. 134-139 ◽  
Author(s):  
John P. Chang ◽  
Janelle Graeter ◽  
Kevin J. Catt
Keyword(s):  
Arachidonic Acid ◽  
Protein Kinase C ◽  
Protein Kinase ◽  
Luteinizing Hormone ◽  
Gonadotropin Releasing Hormone ◽  
Releasing Hormone ◽  
Kinase C

Mechanism of action of luteinizing hormone-releasing hormone in rat ovarian cells

1989 ◽  
Vol 67 (8) ◽  
pp. 962-967 ◽  
Author(s):  
Peter C. K. Leung ◽  
Jian Wang ◽  
Kenneth G. Baimbridge
Keyword(s):  
Signal Transduction ◽  
Protein Kinase C ◽  
Protein Kinase ◽  
Luteinizing Hormone ◽  
Inositol Phosphates ◽  
Ionophore A23187 ◽  
Luteinizing Hormone Releasing Hormone ◽  
Releasing Hormone ◽  
Ovarian Cells ◽  
Kinase C

The initial step in the signal transduction of luteinizing hormone-releasing hormone (LHRH) in rat ovarian cells is the hydrolysis of membrane polyphosphoinositides into inositol phosphates and 1,2-diacylglycerol. The former compounds, especially inositol 1,4,5-triphosphate, are known to cause the release of calcium from intracellular stores, while diacylglycerol is a potent activator of protein kinase C. LHRH causes a rapid and transient increase in intracellular concentrations of free calcium ions, by approximately 4.5-fold, in the majority of granulosa cells as assessed by fura-2 microspectrofluorimetry. Like LHRH, a calcium ionophore (A23187) and activators of protein kinase C attenuate the steroidogenic response of the cells to follicle-stimulating hormone, but enhance the formation of gonadotropin-induced prostaglandin formation. These results support the concept that stimulation of polyphosphoinositide hydrolysis is intimitely involved in the direct action of LHRH at the level of the ovary.Key words: signal transduction, calcium, protein kinase C, ovary, steroid hormones.

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