Dissociation between ornithine decarboxylase activity and aryl hydrocarbon hydroxylase induction in cell cultures treated with benz[a]anthracene and inhibitors of ornithine decarboxylase

1. Ornithine decarboxylase activity is stimulated in high-density HeLa-cell cultures by dilution of or replacement of spent culture medium with fresh medium containing 10% (v/v) horse serum. 2. After stimulation, ornithine decarboxylase activity reaches a peak at 4–6h, then rapidly declines to the low enzyme activity characteristic of quiescent cultures, where it remains during the remainder of the cell cycle. 3. The stimulation of ornithine decarboxylase is eliminated by the addition of 0.5μm-spermine or -spermidine or 10μm-putrescine to the HeLa-cell cultures at the time of re-feeding with fresh medium. Much higher concentrations (1mm) of the non-physiological diamines, 1,3-diamino-propane or 1,3-diamino-2-hydroxypropane, are required to eliminate the stimulation of ornithine decarboxylase in re-fed HeLa-cell cultures. 4. A heat-labile, non-diffusible inhibitor, comparable with the inhibitory protein ornithine decarboxylase antizyme, is induced in HeLa cells by the addition of exogenous diamines or polyamines. 5. Intracellular putrescine is eliminated, intracellular spermidine and spermine are severely decreased and proliferation of HeLa cells is inhibited when cultures are maintained for 48h in the presence of the non-physiological inducer of ornithine decarboxylase antizyme, 1,3-diamino-2-hydroxypropane. Exogenous putrescine, a physiological inducer of the antizyme, does not decrease intracellular polyamines or interfere with proliferation of HeLa cells.

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Phorbol esters stimulate DNA synthesis and ornithine decarboxylase activity in mouse epidermal cell cultures

Nature ◽

10.1038/262402a0 ◽

1976 ◽

Vol 262 (5567) ◽

pp. 402-404 ◽

Cited By ~ 156

Author(s):

STUART H. YUSPA ◽

ULRIKE LICHTI ◽

THERESA BEN ◽

ELROY PATTERSON ◽

HENRY HENNINGS ◽

...

Keyword(s):

Dna Synthesis ◽

Ornithine Decarboxylase ◽

Epidermal Cell ◽

Cell Cultures ◽

Phorbol Esters ◽

Decarboxylase Activity ◽

Ornithine Decarboxylase Activity ◽

Mouse Epidermal Cell

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PPAR-γ ligands induce G1 cell cycle arrest and reduce ornithine decarboxylase activity of human Barrett's adenocarcinoma cells

Gastroenterology ◽

10.1016/s0016-5085(01)82062-x ◽

2001 ◽

Vol 120 (5) ◽

pp. A415-A415

Author(s):

T TAKASHIMA ◽

Y FUJIWARA ◽

K HIGUCHI ◽

T ARAKAWA ◽

Y YANO ◽

...

Keyword(s):

Cell Cycle ◽

Cell Cycle Arrest ◽

Ornithine Decarboxylase ◽

Decarboxylase Activity ◽

Ornithine Decarboxylase Activity ◽

Ppar Γ ◽

Cycle Arrest ◽

Adenocarcinoma Cells ◽

Barrett’S Adenocarcinoma ◽

G1 Cell Cycle Arrest

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Ornithine Decarboxylase Activity in Cultured Endothelial Cells Stimulated by Serum, Thrombin and Serotonin

Thrombosis and Haemostasis ◽

10.1055/s-0038-1646709 ◽

1978 ◽

Vol 39 (02) ◽

pp. 496-503 ◽

Cited By ~ 8

Author(s):

P A D’Amore ◽

H B Hechtman ◽

D Shepro

Keyword(s):

Endothelial Cells ◽

Ornithine Decarboxylase ◽

Decarboxylase Activity ◽

Aortic Endothelial Cells ◽

Ornithine Decarboxylase Activity ◽

Rate Limiting Step ◽

Bovine Aortic Endothelial Cells ◽

Limiting Step ◽

Rate Limiting ◽

Serum Serotonin

SummaryOrnithine decarboxylase (ODC) activity, the rate-limiting step in the synthesis of polyamines, can be demonstrated in cultured, bovine, aortic endothelial cells (EC). Serum, serotonin and thrombin produce a rise in ODC activity. The serotonin-induced ODC activity is significantly blocked by imipramine (10-5 M) or Lilly 11 0140 (10-6M). Preincubation of EC with these blockers together almost completely depresses the 5-HT-stimulated ODC activity. These observations suggest a manner by which platelets may maintain EC structural and metabolic soundness.

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